Pseudofulvibacter gastropodicola sp. nov., isolated from a marine conch and emended descriptions of the genus Pseudofulvibacter Yoon et al. 2013 and Pseudofulvibacter geojedonensis
A Gram-stain-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC08749T, was isolated from a marine conch collected at Samcheok Port in the East Sea, Korea (also known as the Sea of Japan). 16S rRNA gene sequence analysis revealed that strain MEBiC08749T shared highest similarity with Pseudofulvibacter geojedonensis YCS-9T (94.9 %). Growth was observed at 5–31 °C (optimum, 25 °C), at pH 6.0–8.5 (optimum, pH 7.5) and with 0–6 % (w/v) NaCl (optimum, 2.5 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 9 (comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c). The DNA G+C content was 30 mol%. The only respiratory quinone was menaquinone-6 (MK-6). Phosphatidylethanolamine, two unidentified lipids and two unidentified aminolipids were detected as major polar lipids. Several phenotypic characteristics, such as production of acetoin, activity of cystine arylamidase, utilization of some carbohydrates, organic acids and amino acids, differentiated strain MEBiC08749T from P. geojedonensis KCTC 23884T. On the basis of data from this polyphasic taxonomic study, strain MEBiC08749T should be classified as representing a novel species in the genus Pseudofulvibacter, for which the name Pseudofulvibacter gastropodicola sp. nov. is proposed. The type strain is MEBiC08749T ( = KCCM 43046T = JCM 30369T). Emended descriptions of the genus PseudofulvibacterYoon et al. 2013 and of Pseudofulvibacter geojedonensis are also given.
Acosta-GonzálezA., Rosselló-MóraR., MarquésS.2013; Characterization of the anaerobic microbial community in oil-polluted subtidal sediments: aromatic biodegradation potential after the Prestige oil spill. Environ Microbiol 15:77–92 [View Article][PubMed]
AndersonR. E., BeltránM. T., HallamS. J., BarossJ. A.2013; Microbial community structure across fluid gradients in the Juan de Fuca Ridge hydrothermal system. FEMS Microbiol Ecol 83:324–339 [View Article][PubMed]
BaiJ., LiH., ZhaoY.2009; [Bacterial distribution at different stations in the Northern Yellow Sea]. Wei Sheng Wu Xue Bao 49:343–350 (in Chinese). [PubMed]
CollinsM. D.1985; Isoprenoid quinone analysis in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287Edited byGoodfellowM., MinikinD. E. London: Academic Press;
DittmerJ. C. F., LesterR. L.1964; A simple specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 5:126–127[PubMed]
GarrityG. M., HoltJ. G.2001; The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp 119–166Edited byBooneD. R., CastenholzR. W., GarrityG. M. New York: Springer; [View Article]
KwonK. K., LeeH.-S., YangS. H., KimS.-J.2005; Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. Int J Syst Evol Microbiol 55:2033–2037 [View Article][PubMed]
LeeJ.-W., KwonK. K., AziziA., OhH.-M., KimW., BahkJ.-J., LeeD.-H., LeeJ.-H.2013; Microbial community structures of methane hydrate bearing sediments in the Ulleung Basin, East Sea of Korea. Mar Pet Geol 47:136–146 [View Article]
MinnikinD. E., O'DonnellA. G., GoodfellowM., AldersonG., AthalyeM., SchaalA., ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipid. J Microbiol Methods 2:233–241 [View Article]
SchauerR., BienholdC., RametteA., HarderJ.2010; Bacterial diversity and biogeography in deep-sea surface sediments of the South Atlantic Ocean. ISME J 4:159–170 [View Article][PubMed]
SmibertR. M., KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited byGerhardtP., MurrayR. G. E., WoodW. A., KriegN. R. Washington, DC: American Society for Microbiology;
StackebrandtE., LiesackW.1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp 158–160Edited byGoodfellowM., O'DonnellA. G. London: Academic Press;
TamaokaJ., KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
YangS.-H., SeoH.-S., OhH.-M., KimS.-J., LeeJ.-H., KwonK. K.2013; Brumimicrobium mesophilum sp. nov., isolated from a tidal flat sediment, and emended descriptions of the genus Brumimicrobium and Brumimicrobium glaciale
. Int J Syst Evol Microbiol 63:1105–1110 [View Article][PubMed]
YoonJ.-H., LeeM.-H., JungY.-T.2013; Pseudofulvibacter geojedonensis gen. nov., sp. nov., a polysaccharide-degrading member of the family Flavobacteriaceae isolated from seawater, and emended description of the genus Fulvibacter
. Int J Syst Evol Microbiol 63:1696–1701 [View Article][PubMed]
Pseudofulvibacter gastropodicola sp. nov., isolated from a marine conch and emended descriptions of the genus Pseudofulvibacter Yoon et al. 2013 and Pseudofulvibacter geojedonensis