1887

Abstract

Three bacterial strains, HKU51, HKU52 and HKU53, were isolated from a conjunctival swab, corneal scraping and blood culture of three patients in Hong Kong with conjunctivitis, keratitis and catheter-related bacteraemia, respectively. Cells were Gram-stain-positive, aerobic, catalase-positive, non-sporulating and non-motile bacilli. The three strains had unique biochemical profiles that were distinguishable from those of closely related species of the genus . Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of . 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU52 and HKU53, and the two strains shared 99.5 % sequence identity with JCM 15929 and JCM 13375; HKU51 shared 99.6 % sequence identity with CCUG 35732. The DNA G+C contents of strains HKU51, HKU52 and HKU53 were 70.9 ± 2.2, 71.3 ± 2.1 and 71.2 ± 2.3 mol% (mean ± ;  = 3), respectively. DNA–DNA hybridization confirmed that the novel strains were distinct from other known species of the genus ( ≤ 50.1 ± 3.7 % DNA–DNA relatedness); two of the isolates, HKU52 and HKU53, represented the same species ( ≥ 94.6 ± 5.6 % DNA–DNA relatedness), while the third isolate, HKU51, represented another species. The novel species sp. nov. is proposed to accommodate strains HKU52 and HKU53, with HKU52 ( = JCM 30715 = DSM 100208) as the type strain; whilst another novel species, sp. nov., is proposed to accommodate the third isolate, HKU51 ( = JCM 30714 = DSM 100207), which is designated the type strain.

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2016-01-01
2019-12-12
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References

  1. Abramoff M. D., Magalhaes P. J., Ram S. J.. 2004; Image processing with ImageJ. Biophotonics Int11:36–42
    [Google Scholar]
  2. Alcaide M. L., Espinoza L., Abbo L.. 2004; Cavitary pneumonia secondary to Tsukamurella in an AIDS patient. First case and a review of the literature. J Infect49:17–19 [CrossRef][PubMed]
    [Google Scholar]
  3. Almehmi A., Pfister A. K., McCowan R., Matulis S.. 2004; Implantable cardioverter-defibrillator infection caused by Tsukamurella. W V Med J100:185–186[PubMed]
    [Google Scholar]
  4. Bouza E., Pérez-Parra A., Rosal M., Martín-Rabadán P., Rodríguez-Créixems M., Marín M.. 2009; Tsukamurella: a cause of catheter-related bloodstream infections. Eur J Clin Microbiol Infect Dis28:203–210 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Smida J., Dorsch M., Stackebrandt E.. 1988; Tsukamurella gen. nov., harboring Corynebacterium paurometabolum and Rhodococcus aurantiacus. Int J Syst Bacteriol38:385–391 [CrossRef]
    [Google Scholar]
  6. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  7. Hall T. A.. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser41:95–98
    [Google Scholar]
  8. Kattar M. M., Cookson B. T., Carlson L. D., Stiglich S. K., Schwartz M. A., Nguyen T. T., Daza R., Wallis C. K., Yarfitz S. L., Coyle M. B.. 2001; Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. J Clin Microbiol39:1467–1476 [CrossRef][PubMed]
    [Google Scholar]
  9. Larkin J. A., Lit L., Sinnott J., Wills T., Szentivanyi A.. 1999; Infection of a knee prosthesis with Tsukamurella species. South Med J92:831–832 [CrossRef][PubMed]
    [Google Scholar]
  10. Lau S. K. P., McNabb A., Woo G. K. S., Hoang L., Fung A. M. Y., Chung L. M. W., Woo P. C. Y., Yuen K.-Y.. 2007; Catabacter hongkongensis gen. nov., sp. nov., isolated from blood cultures of patients from Hong Kong and Canada. J Clin Microbiol45:395–401 [CrossRef][PubMed]
    [Google Scholar]
  11. Lau S. K. P., Curreem S. O. T., Lin C. C. N., Fung A. M. Y., Yuen K.-Y., Woo P. C. Y.. 2013; Streptococcus hongkongensis sp. nov., isolated from a patient with an infected puncture wound and from a marine flatfish. Int J Syst Evol Microbiol63:2570–2576 [CrossRef][PubMed]
    [Google Scholar]
  12. Liu C. Y., Lai C. C., Lee M. R., Lee Y. C., Huang Y. T., Liao C. H., Hsueh P. R.. 2011; Clinical characteristics of infections caused by Tsukamurella spp. and antimicrobial susceptibilities of the isolates. Int J Antimicrob Agents38:534–537 [CrossRef][PubMed]
    [Google Scholar]
  13. Marmur J., Doty P.. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5:109–118 [CrossRef][PubMed]
    [Google Scholar]
  14. Park S. W., Kim S. M., Park S. T., Kim Y. M.. 2009; Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete. Int J Syst Evol Microbiol59:1541–1544 [CrossRef][PubMed]
    [Google Scholar]
  15. Perez V. A. J., Swigris J., Ruoss S. J.. 2008; Coexistence of primary adenocarcinoma of the lung and Tsukamurella infection: a case report and review of the literature. J Med Case Reports2:207 [CrossRef][PubMed]
    [Google Scholar]
  16. Rhuland L. E., Work E., Denman R. F., Hoare D. S.. 1955; The behavior of the isomers of α,-diaminopimelic acid on paper chromatograms. J Am Chem Soc77:4844–4846 [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  18. Sasser M.. 1990; Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology pp199–204Edited by Klement Z., Rudolph K., Sands D. C.. Budapest: Akademiai Kiado;
    [Google Scholar]
  19. Savini V., Fazii P., Favaro M., Astolfi D., Polilli E., Pompilio A., Vannucci M., D'Amario C., Di Bonaventura G., other authors. 2012; Tuberculosis-like pneumonias by the aerobic actinomycetes Rhodococcus, Tsukamurella and Gordonia. Microbes Infect14:401–410 [CrossRef][PubMed]
    [Google Scholar]
  20. Schwartz M. A., Tabet S. R., Collier A. C., Wallis C. K., Carlson L. C., Nguyen T. T., Kattar M. M., Coyle M. B.. 2002; Central venous catheter-related bacteremia due to Tsukamurella species in the immunocompromised host: a case series and review of the literature. Clin Infect Dis35:e72–e77 [CrossRef][PubMed]
    [Google Scholar]
  21. Seong C. N., Kim Y. S., Baik K. S., Choi S. K., Kim M. B., Kim S. B., Goodfellow M.. 2003; Tsukamurella sunchonensis sp. nov., a bacterium associated with foam in activated sludge. J Microbiol41:83–88
    [Google Scholar]
  22. Shaer A. J., Gadegbeku C. A.. 2001; Tsukamurella peritonitis associated with continuous ambulatory peritoneal dialysis. Clin Nephrol56:241–246[PubMed]
    [Google Scholar]
  23. Sheng W.-H., Huang Y.-T., Chang S.-C., Hsueh P.-R.. 2009; Brain abscess caused by Tsukamurella tyrosinosolvens in an immunocompetent patient. J Clin Microbiol47:1602–1604 [CrossRef][PubMed]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  26. Steinhaus E. A.. 1941; A study of the bacteria associated with thirty species of insects. J Bacteriol42:757–790[PubMed]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  28. To K. K. W., Fung A. M. Y., Teng J. L. L., Curreem S. O. T., Lee K.-C., Yuen K.-Y., Lam C.-W., Lau S. K. P., Woo P. C. Y.. 2013; Characterization of a Tsukamurella pseudo-outbreak by phenotypic tests, 16S rRNA sequencing, pulsed-field gel electrophoresis, and metabolic footprinting. J Clin Microbiol51:334–338 [CrossRef][PubMed]
    [Google Scholar]
  29. Tsukamura M., Mizuno S.. 1971; [A new species Gordona aurantiaca occurring in sputa of patients with pulmonary disease]. Kekkaku46:93–98 (in Japanese).[PubMed]
    [Google Scholar]
  30. Woo P. C. Y., Tam D. M. W., Leung K.-W., Lau S. K. P., Teng J. L. L., Wong M. K. M., Yuen K.-Y.. 2002; Streptococcus sinensis sp. nov., a novel species isolated from a patient with infective endocarditis. J Clin Microbiol40:805–810 [CrossRef][PubMed]
    [Google Scholar]
  31. Woo P. C. Y., Ngan A. H. C., Lau S. K. P., Yuen K.-Y.. 2003a; Tsukamurella conjunctivitis: a novel clinical syndrome. J Clin Microbiol41:3368–3371 [CrossRef][PubMed]
    [Google Scholar]
  32. Woo P. C. Y., Fung A. M. Y., Lau S. K. P., Teng J. L. L., Wong B. H. L., Wong M. K. M., Hon E., Tang G. W. K., Yuen K.-Y.. 2003b; Actinomyces hongkongensis sp. nov. A novel Actinomyces species isolated from a patient with pelvic actinomycosis. Syst Appl Microbiol26:518–522 [CrossRef][PubMed]
    [Google Scholar]
  33. Woo P. C. Y., Fung A. M. Y., Lau S. K. P., Chan B. Y. L., Chiu S. K., Teng J. L. L., Que T. L., Yung R. W. H., Yuen K.-Y.. 2003c; Granulicatella adiacens and Abiotrophia defectiva bacteraemia characterized by 16S rRNA gene sequencing. J Med Microbiol52:137–140 [CrossRef][PubMed]
    [Google Scholar]
  34. Woo P. C. Y., Tse H., Lau S. K. P., Leung K.-W., Woo G. K. S., Wong M. K. M., Ho C.-M., Yuen K.-Y.. 2005; Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess. Syst Appl Microbiol28:316–322 [CrossRef][PubMed]
    [Google Scholar]
  35. Woo P. C. Y., Fong A. H. C., Ngan A. H. Y., Tam D. M. W., Teng J. L. L., Lau S. K. P., Yuen K.-Y.. 2009; First report of Tsukamurella keratitis: association between T. tyrosinosolvens and T. pulmonis and ophthalmologic infections. J Clin Microbiol47:1953–1956 [CrossRef][PubMed]
    [Google Scholar]
  36. Woo P. C. Y., Wu A. K. L., Tsang C.-C., Leung K.-W., Ngan A. H. Y., Curreem S. O. T., Lam K. W., Chen J. H. K., Chan J. F., Lau S. K.. 2014; Streptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis. Int J Syst Evol Microbiol64:3034–3039 [CrossRef][PubMed]
    [Google Scholar]
  37. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Lee H. J., Schaal K. P.. 1995; Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol45:522–527 [CrossRef][PubMed]
    [Google Scholar]
  38. Yassin A. F., Rainey F. A., Brzezinka H., Burghardt J., Rifai M., Seifert P., Feldmann K., Schaal K. P.. 1996; Tsukamurella pulmonis sp. nov. Int J Syst Bacteriol46:429–436 [CrossRef][PubMed]
    [Google Scholar]
  39. Yassin A. F., Rainey F. A., Burghardt J., Brzezinka H., Schmitt S., Seifert P., Zimmermann O., Mauch H., Gierth D., other authors. 1997; Tsukamurella tyrosinosolvens sp. nov. Int J Syst Bacteriol47:607–614 [CrossRef][PubMed]
    [Google Scholar]
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