A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium designated FJAT-4402T, was isolated from the weed rhizosphere soil of the Gobi desert in the Xinjiang Autonomous Region in the north-west of China. Isolate FJAT-4402T grew at 15–40 °C (optimum 30 °C), pH 5–10 (optimum pH 7) and in 0–3 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that isolate FJAT-4402T was a member of the genus Bacillus and was most closely related to Bacillus licheniformis DSM 13T (96.2 %). The isolate showed 33.3 % DNA–DNA relatedness to the closest reference isolate, B. licheniformis DSM 13T. The diagnostic diamino acid of the peptidoglycan of isolate FJAT-4402T was meso-diaminopimelic acid and the predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0 (28.5 %), iso-C15 : 0 (20.1 %), anteiso-C17 : 0 (14.3 %), iso-C16 : 0 (9.6 %), C16 : 0 (8.4 %), iso-C17 : 0 (6.2 %) and iso-C14 : 0 (4.7 %) and the DNA G+C content was 42.0 mol%. The phenotypic, chemotaxonomic and genotypic properties indicated that strain FJAT-4402T represents a novel species within the genus Bacillus, for which the name Bacillus gobiensis sp. nov. is proposed. The type strain is FJAT-4402T ( = DSM 29500T = CGMCC 1.12902T).
CollinsM. D., PirouzT., GoodfellowM., MinnikinD. E.1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [View Article][PubMed]
De LeyJ., CattoirH., ReynaertsA.1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
GrothI., SchumannP., WeissN., MartinK., RaineyF. A.1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
HopwoodD. A., BibbM. J., ChaterK. F., KieserT., BrutonC. J., KieserH. M., LydiateD. J., SmithC. P., WardJ. M., SchrempfH.editors1985Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;
HussV. A., FestlH., SchleiferK. H.1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
JukesT. H., CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolismvol. 3 pp 21–132Edited byMunroH. N. New York: Academic Press; [View Article]
KämpferP.1994; Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. Syst Appl Microbiol 17:86–98 [View Article]
LoganN. A., De VosP.2009Genus I. Bacillus. In Bergey's Manual of Systematic Bacteriologyvol. 3 pp 21–128Edited byDe VosP., GarrityG., JonesD., KriegN. R., LudwigW., RaineyF. A., SchleiferK. H., WhitmanW. B. New York: Springer;
LoganN. A., BergeO., BishopA. H., BusseH. J., De VosP., FritzeD., HeyndrickxM., KämpferP., RabinovitchL., other authors. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [View Article][PubMed]
MesbahM., PremachandranU., WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E., CollinsM. D., GoodfellowM.1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [View Article]
MurrayR. G. E., DoetschR. N., RobinowC. F.1994; Determinative and cytological light microscopy. In Methods for general and molecular bacteriology pp 21–41Edited byGerhardtP., MurrayR. G. E., WoodW. A., KriegN. R. Washington: American Society for Microbiology;
SmibertR. M., KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–655Edited byGerhardtP., MurrayR. G. E., WoodW. A., KriegN. R. Washington, DC: American Society for Microbiology;
StackebrandtE., GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
ThompsonJ. D., GibsonT. J., PlewniakF., JeanmouginF., HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
WayneL. G., BrennerD. J., ColwellR. R., GrimontP. A. D., KandlerO., KrichevskyM. I., MooreL. H., MooreW. E. C., MurrayR. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]