1887

Abstract

A rod-shaped, Gram-stain-negative, slightly halotolerant bacterium, designated strain D15-8P, was isolated from a sediment sample from the South China Sea. The strain could grow in NaCl concentrations ranging from 0.5 % to 10 % (w/v) (optimum 0.5–1.5 %), and could be cultivated at 10–40 °C (optimum 25 °C) and pH 5.5–9.5 (optimum pH 7.0–8.0). The strain was positive for catalase, oxidase, and hydrolysis of Tween 80, but negative for hydrolysis of DNA and gelatin, nitrite reduction, indole production, Voges–Proskauer reaction, and methyl red test. Strain D15-8P could biodegrade naphthalene, phenanthrene, and anthracene. The major respiratory quinone was Q-9. The main cellular fatty acids were C (11.5 %), C 3-methyl (22.0 %), C (19.2 %), Cω9 (22.9 %), and Cω9 (6.7 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid and an unidentified phospholipid. The DNA G+C content was 56.8 mol%. Phylogenetic analyses based on 16S rRNA genes showed that strain D15-8P was most closely related to JCM 12521 (98.5 % 16S rRNA gene sequence similarity), CGMCC 1.10835 (98.1 %), DSM 15157 (97.1 %), and CECT 7243 (97.0 %). Results of the gene analysis and DNA–DNA hybridization were both less than the cut-off values (90 % for gene sequence similarity and 70 % for DNA–DNA hybridization). On the basis of this taxonomic study using a polyphasic approach, strain D15-8P represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D15-8P ( = CGMCC 1.11015 = KCTC 23781).

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2016-01-01
2019-10-23
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