1887

Abstract

A novel actinomycete, strain RCU-197, was isolated from soil of a peat swamp forest in Rayong Province, Thailand. Using a polyphasic approach, the strain was classified in the genus . It contained -diaminopimelic acid in the cell-wall peptidoglycan. No diagnostic sugars were detected in whole-cell hydrolysates and there was a lack of mycolic acids. The major menaquinones were MK-9(H) and MK-9(H). The predominant cellular fatty acids were iso-C, iso-C, anteiso-C and iso-C. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannoside, an unknown aminolipid and two unknown phospholipids. Phylogenetic analysis of 16S rRNA gene sequences showed the strain formed distinct clade within the genus and was closely related to NBRC 12763 (98.78 % 16S rRNA gene sequence similarity). According to the polyphasic approach as well as DNA–DNA relatedness, the strain could be clearly differentiated from closely related species and represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RCU-197 ( = JCM 30864 = TISTR 2208 = PCU 342).

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2016-01-01
2020-09-19
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