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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 1

Group 16SrXI phytoplasma strains, including subgroup 16SrXI-B and a new subgroup, 16SrXI-D, are associated with sugar cane white leaf Free

Abstract

Sugar cane white leaf (SCWL) is a serious disease caused by phytoplasmas. In this study, we performed nested PCR with phytoplasma universal primer pairs (P1/P7 and R16F2n/R16R2) for the 16S rRNA gene to detect SCWL phytoplasmas in 31 SCWL samples collected from Baoshan and Lincang, Yunnan, China. We cloned and sequenced the nested PCR products, revealing that the 16S rRNA gene sequences from 31 SCWL samples were all 1247 bp in length and shared more than 99 % nucleotide sequence similarity with the 16S rRNA gene sequences of SCWL phytoplasmas from various countries. Based on the reported 16S rRNA gene sequence data from SCWL isolates of various countries, we conducted phylogenetic and virtual RFLP analysis. In the resulting phylogenetic tree, all SCWL isolates clustered into two branches, with the Lincang and Baoshan SCWL phytoplasma isolates belonging to different branches. The virtual RFLP patterns show that phytoplasmas of the Lincang branch belong to subgroup 16SrXI-B. However, the virtual RFLP patterns revealed by III digestion of phytoplasmas of the Baoshan branch differed from those of subgroup 16SrXI-B. According to the results of phylogenetic and virtual RFLP analysis, we propose that the phytoplasmas of the Baoshan branch represent a new subgroup, 16SrXI-D. These findings suggest that SCWL is caused by phytoplasmas from group 16SrXI, including subgroup 16SrXI-B and a new subgroup, 16SrXI-D.

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2016-01-01
2024-04-19
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References

  1. Hanboonsong Y., Choosai C., Panyim S., Damak S. 2002; Transovarial transmission of sugarcane white leaf phytoplasma in the insect vector Matsumuratettix hiroglyphicus (Matsumura). Insect Mol Biol 11:97–103 [View Article][PubMed]
     [Google Scholar]
  2. Hanboonsong Y., Ritthison W., Choosai C., Sirithorn P. 2006; Transmission of sugarcane white leaf phytoplasma by Yamatotettix flavovittatus, a new leafhopper vector. J Econ Entomol 99:1531–1537 [View Article][PubMed]
     [Google Scholar]
  3. Jung H. Y., Sawayanagi T., Wongkaew P., Kakizawa S., Nishigawa H., Wei W., Oshima K., Miyata S., Ugaki M., other authors. 2003; “Candidatus Phytoplasma oryzae”, a novel phytoplasma taxon associated with rice yellow dwarf disease. Int J Syst Evol Microbiol 53:1925–1929 [View Article][PubMed]
     [Google Scholar]
  4. Kumarasinghe N. C., Jones P. 2001; Identification of white leaf disease of sugarcane in Sri Lanka. Sugar Tech 3:55–58 [View Article]
     [Google Scholar]
  5. Lee I. M., Pastore M., Vibio M., Danielli A., Attathom S., Davis R. E., Bertaccini A. 1997; Detection and characterization of a phytoplasma associated with annual blue grass (Poa annua) white leaf disease in southern Italy. Eur J Plant Pathol 103:251–254 [View Article]
     [Google Scholar]
  6. Lee I. M., Gundersen-Rindal D. E., Davis R. E., Bartoszyk I. M. 1998; Revised classification scheme of phytoplasma based on RFLP analyses of 16S rDNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169 [View Article]
     [Google Scholar]
  7. Lee I. M., Davis R. E., Gundersen-Rindal D. E. 2000; Phytoplasma: phytopathogenic mollicutes. Annu Rev Microbiol 54:221–255 [View Article][PubMed]
     [Google Scholar]
  8. Lee I. M., Bottner-Parker K. D., Zhao Y., Davis R. E., Harrison N. A. 2010; Phylogenetic analysis and delineation of phytoplasmas based on secY gene sequences. Int J Syst Evol Microbiol 60:2887–2897 [View Article][PubMed]
     [Google Scholar]
  9. Li W. F., Wang X. Y., Huang Y. K., Shen K., Shan H. L., Luo Z. M., Yin J., Jia Y. M., Bai Z. G., Zhang R. Y. 2013; First report of sugarcane white leaf phytoplasma in Yunnan province, China. Can J Plant Pathol 35:407–410 [View Article]
     [Google Scholar]
  10. Madhupriya, Rao G. P., Khurana S. M. P. 2015; Rice yellow dwarf phytoplasma (16SrXI-B subgroup) infecting Jasminum sambac in India. Phytoparasitica 43:77–80 [View Article]
     [Google Scholar]
  11. Marcone C. 2002; Phytoplasma diseases of sugarcane. Sugar Tech 4:79–85 [View Article]
     [Google Scholar]
  12. Martini M., Lee I. M., Bottner K. D., Zhao Y., Botti S., Bertaccini A., Harrison N. A., Carraro L., Marcone C., other authors. 2007; Ribosomal protein gene-based phylogeny for finer differentiation and classification of phytoplasmas. Int J Syst Evol Microbiol 57:2037–2051 [View Article][PubMed]
     [Google Scholar]
  13. Namba S., Oyaizu H., Kato S., Iwanami S., Tsuchizaki T. 1993; Phylogenetic diversity of phytopathogenic mycoplasmalike organisms. Int J Syst Bacteriol 43:461–467 [View Article][PubMed]
     [Google Scholar]
  14. Rao G. P., Singh A., Singh H. B., Sharma S. R. 2005; Phytoplasma diseases of sugarcane: characterization, diagnosis and management. Iranian J Plant Pathol 23:1–21
     [Google Scholar]
  15. Schneider B., Gibb K. S. 1997; Sequence and RFLP analysis of the elongation factor Tu gene used in differentiation and classification of phytoplasmas. Microbiology 143:3381–3389 [View Article][PubMed]
     [Google Scholar]
  16. Smart C. D., Schneider B., Blomquist C. L., Guerra L. J., Harrison N. A., Ahrens U., Lorenz K.-H., Seemüller E., Kirkpatrick B. C. 1996; Phytoplasma-specific PCR primers based on sequences of the 16S–23S rRNA spacer region. Appl Environ Microbiol 62:2988–2993[PubMed]
     [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  18. Thein M. M., Jamjanya T., Kobori Y., Hanboonsong Y. 2012; Dispersal of the leafhoppers Matsumuratettix hiroglyphicus and Yamatotettix flavovittatus (Homoptera: Cicadellidae), vectors of sugarcane white leaf disease. Appl Entomol Zool 47:255–262 [View Article]
     [Google Scholar]
  19. Wang X. Y., Li W. F., Huang Y. K., Zhang R. Y., Luo Z. M., Shan H. L., Yin J., Shen K., Jia Y. M., Bai Z. G. 2014; Analyses of the 16S–23S intergenic region of the phytoplasma causing the sugarcane white leaf disease in Yunnan Province, China. Trop Plant Pathol 39:184–188 [View Article]
     [Google Scholar]
  20. Wei W., Davis R. E., Lee I. M., Zhao Y. 2007; Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Evol Microbiol 57:1855–1867 [View Article][PubMed]
     [Google Scholar]
  21. Wei W., Lee I. M., Davis R. E., Suo X., Zhao Y. 2008; Automated RFLP pattern comparison and similarity coefficient calculation for rapid delineation of new and distinct phytoplasma 16Sr subgroup lineages. Int J Syst Evol Microbiol 58:2368–2377 [View Article][PubMed]
     [Google Scholar]
  22. Zhao Y., Wei W., Lee I. M., Shao J., Suo X., Davis R. E. 2009; Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59:2582–2593 [View Article][PubMed]
     [Google Scholar]
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Group 16SrXI phytoplasma strains, including subgroup 16SrXI-B and a new subgroup, 16SrXI-D, are associated with sugar cane white leaf
Int J Syst Evol Microbiol 66, 487 (2016); https://doi.org/10.1099/ijsem.0.000712
/content/journal/ijsem/10.1099/ijsem.0.000712
/content/journal/ijsem/10.1099/ijsem.0.000712
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