1887

Abstract

The analysis of the bacterial microbiota of retain samples of pork salami revealed an isolate (strain TMW 1.2011) that could neither be assigned to typical genera of starter organisms nor to any other known meat-associated species. Cells were Gram-stain-positive, short, straight rods occurring singly, in pairs or short chains. Phylogenetic analysis of the 16S rRNA gene sequence and specific phenotypic characteristics showed that strain TMW 1.2011 belonged to the phylogenetic group, and the closest neighbours were JCM 14932 (97.8 % 16S rRNA gene sequence similarity), DSM 20183 (97.4 %), ‘’ EMML 3041 (97.3 %), DSM 14857 (96.9 %) and JCM 18764 (97.2 %). Similarities using partial gene sequences of the alternative chronometers , and also support these relationships. DNA–DNA relatedness between the novel isolate and JCM 14932, DSM 14857 and DSM 20183, JCM 18764 and ‘’ EMML 3041 were below 70 % and the DNA G+C content was 36.3 mol%. The cell-wall peptidoglycan type is -Lys-Gly--Asp. Based on phylogenetic, chemotaxonomic and physiological evidence, strain TMW 1.2011 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TMW 1.2011 ( = CECT 8802 = DSM 29801).

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2016-01-01
2024-12-02
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References

  1. Aquilanti L., Santarelli S., Silvestri G., Osimani A., Petruzzelli A., Clementi F. 2007; The microbial ecology of a typical Italian salami during its natural fermentation. Int J Food Microbiol 120:136–145 [View Article][PubMed]
    [Google Scholar]
  2. Aymerich T., Martín B., Garriga M., Hugas M. 2003; Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic Staphylococci from artisanal low-acid sausages. Appl Environ Microbiol 69:4583–4594 [View Article][PubMed]
    [Google Scholar]
  3. Bacus J. N. 1986; Fermented meat and poultry products. In Advances in Meat and Poultry Microbiology pp 123–164Edited by Pearson A. M. D. London: Macmillan;
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  5. Chenoll E., Carmen Macián M., Aznar R. 2006; Lactobacillus tucceti sp. nov., a new lactic acid bacterium isolated from sausage. Syst Appl Microbiol 29:389–395 [View Article][PubMed]
    [Google Scholar]
  6. Cocolin L., Dolci P., Rantsiou K. 2011; Biodiversity and dynamics of meat fermentations: the contribution of molecular methods for a better comprehension of a complex ecosystem. Meat Sci 89:296–302 [View Article][PubMed]
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  8. De Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135 [View Article]
    [Google Scholar]
  9. Demeyer D. I., Verplaetse A., Gistelinck M. 1986; Fermentation of meat: an integrated process. Belgian J Food Chem and Biotechnol 41:131–139
    [Google Scholar]
  10. Ehrmann M. A., Müller M. R. A., Vogel R. F. 2003; Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov. Int J Syst Evol Microbiol 53:7–13 [View Article][PubMed]
    [Google Scholar]
  11. Fadda S., López C., Vignolo G. 2010; Role of lactic acid bacteria during meat conditioning and fermentation: peptides generated as sensorial and hygienic biomarkers. Meat Sci 86:66–79 [View Article][PubMed]
    [Google Scholar]
  12. Hammes W. P., Knauf H. J. 1994; Starters in the processing of meat products. Meat Sci 36:155–168 [View Article][PubMed]
    [Google Scholar]
  13. Hammes W. P., Bantleon A., Min S. 1990; Lactic acid bacteria in meat fermentation. FEMS Microbiol Rev 7:165–174 [View Article]
    [Google Scholar]
  14. Huang C. H., Lee F. L. 2011; The dnaK gene as a molecular marker for the classification and discrimination of the Lactobacillus casei group. Antonie van Leeuwenhoek 99:319–327 [View Article][PubMed]
    [Google Scholar]
  15. Huang C.-H., Lee F.-L., Liou J.-S. 2010; Rapid discrimination and classification of the Lactobacillus plantarum group based on a partial dnaK sequence and DNA fingerprinting techniques. Antonie van Leeuwenhoek 97:289–296 [View Article][PubMed]
    [Google Scholar]
  16. Huss V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  17. Irisawa T., Tanaka N., Kitahara M., Sakamoto M., Ohkuma M., Okada S. 2014; Lactobacillus furfuricola sp. nov., isolated from Nukadoko, rice bran paste for Japanese pickles. Int J Syst Evol Microbiol 64:2902–2906 [View Article][PubMed]
    [Google Scholar]
  18. Jung H. M., Liu Q.M, Kim J. K., Lee S. T., Kim S. C., Im W. T. 2013; Lactobacillus ginsenosidimutans sp. nov., isolated from kimchi with the ability to transform ginsenosides. A Van Leeuwenhoek 103:867–876 [View Article][PubMed]
    [Google Scholar]
  19. Kashiwagi T., Suzuki T., Kamakura T. 2009; Lactobacillus nodensis sp. nov., isolated from rice bran. Int J Syst Evol Microbiol 59:83–86 [View Article][PubMed]
    [Google Scholar]
  20. Kröckel L., Schillinger U., Franz C.M.A.P., Bantleon A., Ludwig W. 2003; Lactobacillus versmoldensis sp. nov., isolated from raw fermented sausage. Int J Syst Evol Microbiol 53:513–517 [View Article][PubMed]
    [Google Scholar]
  21. Leroy F., Verluyten J., De Vuyst L. 2006; Functional meat starter cultures for improved sausage fermentation. Int J Food Microbiol 106:270–285 [View Article][PubMed]
    [Google Scholar]
  22. Mattarelli P., Holzapfel W., Franz C. M., Endo A., Felis G. E., Hammes W., Pot B., Dicks L., Dellaglio F. 2014; Recommended minimal standards for description of new taxa of the genera Bifidobacterium, Lactobacillus and related genera. Int J Syst Evol Microbiol 64:1434–1451 [View Article][PubMed]
    [Google Scholar]
  23. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  24. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. 2005; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150 [View Article][PubMed]
    [Google Scholar]
  25. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J. 2007; Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 57:2777–2789 [View Article][PubMed]
    [Google Scholar]
  26. Pereira C. I., Crespo M. T., Romão M. V. 2001; Evidence for proteolytic activity and biogenic amines production in Lactobacillus curvatus and L. homohiochii . Int J Food Microbiol 68:211–216 [View Article][PubMed]
    [Google Scholar]
  27. Rantsiou K., Cocolin L. 2006; New developments in the study of the microbiota of naturally fermented sausages as determined by molecular methods: a review. Int J Food Microbiol 108:255–267 [View Article][PubMed]
    [Google Scholar]
  28. Reuter G. 1970; Laktobazillen und eng verwandte Mikroorganismen in Fleisch und Fleischerzeugnissen. 2. Mitteilung: Die Charakterisierung der isolierten Laktobazillenstaemme. Fleischwirtschaft 50:954–962
    [Google Scholar]
  29. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [View Article][PubMed]
    [Google Scholar]
  30. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  31. Sanz Y., Fadda S., Vignolo G., Aristoy M.-C., Oliver G., Toldrá F. 1999; Hydrolysis of muscle myofibrillar proteins by Lactobacillus curvatus and Lactobacillus sake . Int J Food Microbiol 53:115–125 [View Article][PubMed]
    [Google Scholar]
  32. Schumann P. 2011; Peptidoglycan structure. Methods Microbiol 38:101–129 [View Article]
    [Google Scholar]
  33. Stackebrandt E., Ebers J. 2006; Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155
    [Google Scholar]
  34. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  35. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  36. Urso R., Comi G., Cocolin L. 2006; Ecology of lactic acid bacteria in Italian fermented sausages: isolation, identification and molecular characterization. Syst Appl Microbiol 29:671–680 [View Article][PubMed]
    [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
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