1887

Abstract

An alkalitolerant actinomycete strain, designated EGI 80425, capable of producing ansamitocin P-3, was isolated from a saline–alkali soil sample of Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80425 formed non-fragmented substrate mycelia and white aerial hyphae with long spore chains. Whole-cell hydrolysates of the isolate contained -diaminopimelic acid as the diagnostic diamino acid and rhamnose as the major sugar. The major fatty acids were anteiso-C, iso-C and Cω9. The predominant menaquinones were MK-10(H), MK-10(H), MK-10(H) and MK-9(H). The G+C content of the genomic DNA of strain EGI 80425 was 70.2 mol%. Strain EGI 80425 showed highest 16S rRNA gene sequence similarity to subsp. DSM 43111 (96.44 %). Phylogenetic analysis showed that strain EGI 80425 clustered with the members of the genus . Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain EGI 80425 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 80425 ( = CGMCC 9969 = KCTC 39605).

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2016-01-01
2019-10-19
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