1887

Abstract

Three Gram-negative, non-spore-forming, encapsulated bacteria were isolated from a Namibian river-bank soil (strains 277 and 307) and a semiarid savannah soil (strain A2-1c). 16S rRNA gene sequence analyses placed them within subdivision 1 of the and revealed 100 % similarity between strains 277 and 307 and 98.2 % similarity between A2-1c and the former two strains. The closest relatives with validly published names were , and (94.7–95.9 % similarity to the type strains). Cells of all three strains were rod-shaped and motile and divided by binary fission. Ultrastructural analyses revealed a thick cell envelope, resulting mainly from a thick periplasmic space. Colonies of strains 277 and 307 were white to cream and light pink, respectively, while strain A2-1c displayed a bright pink colour. All three strains were aerobic, chemoheterotrophic mesophiles with a broad temperature range for growth and a moderately acidic pH optimum. Sugars and complex proteinaceous substrates were the preferred carbon and energy sources. A few polysaccharides were degraded. The major quinone in all three strains was MK-8; MK-7 occurred in strain A2-1c as a minor compound. Major fatty acids were iso-C and iso-Cω7. In addition, iso-C occurred in significant amounts. The DNA G+C contents of strains 277, 307 and A2-1c were 59.6, 59.9 and 58.5 mol%, respectively. Based on these characteristics, the three isolates are assigned to two novel species of the novel genus gen. nov., sp. nov. [type strain 277 ( = DSM 25168 = LMG 26948) and reference strain 307 ( = DSM 25169 = LMG 26947)] and sp. nov. [type strain A2-1c ( = DSM 25170 = LMG 26946)]. Together with several other recently described taxa, the novel isolates provide the basis for an emended description of the established family .

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2016-01-01
2019-09-16
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