sp. nov., isolated from Iberian ibex () Free

Abstract

Biochemical and molecular genetic studies were performed on a novel Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from tonsil samples of two Iberian ibexes. The micro-organism was identified as a streptococcal species based on its cellular, morphological and biochemical characteristics. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus , but the organism did not correspond to any species of this genus. The nearest phylogenetic relative of the unknown coccus from ibex was 2923-03 (96.6 % 16S rRNA gene sequence similarity). Analysis based on and gene sequences revealed sequence similarity values lower than 86.0 and 83.8 %, respectively, from the type strains of recognized species. The novel bacterial isolate was distinguished from and other species using biochemical tests. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DICM07-02790-1C ( = CECT 8872 = CCUG 67170).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000697
2016-01-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/196.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000697&mimeType=html&fmt=ahah

References

  1. Aranaz A., Liébana E., Gómez-Mampaso E., Galán J. C., Cousins D., Ortega A., Blázquez J., Baquero F., Mateos A., other authors. 1999; Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain. Int J Syst Bacteriol 49:1263–1273 [View Article][PubMed]
    [Google Scholar]
  2. Aranaz A., Cousins D., Mateos A., Domínguez L. 2003; Elevation of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium caprae comb. nov., sp. nov. Int J Syst Evol Microbiol 53:1785–1789 [View Article][PubMed]
    [Google Scholar]
  3. Dehio C., Lanz C., Pohl R., Behrens P., Bermond D., Piémont Y., Pelz K., Sander A. 2001; Bartonella schoenbuchii sp. nov., isolated from the blood of wild roe deer. Int J Syst Evol Microbiol 51:1557–1565 [View Article][PubMed]
    [Google Scholar]
  4. Drancourt M., Roux V., Fournier P. E., Raoult D. 2004; rpoB gene sequence-based identification of aerobic Gram-positive cocci of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella . J Clin Microbiol 42:497–504 [View Article][PubMed]
    [Google Scholar]
  5. Facklam R., Elliott J. A. 1995; Identification, classification, and clinical relevance of catalase-negative, gram-positive cocci, excluding the streptococci and enterococci. Clin Microbiol Rev 8:479–495[PubMed]
    [Google Scholar]
  6. Glazunova O. O., Raoult D., Roux V. 2006; Streptococcus massiliensis sp. nov., isolated from a patient blood culture. Int J Syst Evol Microbiol 56:1127–1131 [View Article][PubMed]
    [Google Scholar]
  7. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [View Article][PubMed]
    [Google Scholar]
  8. Jores J., Fischer A., Sirand-Pugnet P., Thomann A., Liebler-Tenorio E. M., Schnee C., Santana-Cruz I., Heller M., Frey J. 2013; Mycoplasma feriruminatoris sp. nov., a fast growing Mycoplasma species isolated from wild Caprinae. Syst Appl Microbiol 36:533–538 [View Article][PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  11. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  12. Page R. D. M. 1996; TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358[PubMed]
    [Google Scholar]
  13. Poyart C., Quesne G., Coulon S., Berche P., Trieu-Cuot P. 1998; Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol 36:41–47[PubMed]
    [Google Scholar]
  14. Rasmussen S. W. 2002; SeqTools, a Program Suite for Sequence Analysis. Copenhagen: Carlsberg Laboratory;
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[PubMed] [CrossRef]
    [Google Scholar]
  17. Vela A. I., Goyache J., Tarradas C., Luque I., Mateos A., Moreno M. A., Borge C., Perea J. A., Domínguez L., Fernández-Garayzábal J. F. 2003; Analysis of genetic diversity of Streptococcus suis clinical isolates from pigs in Spain by pulsed-field gel electrophoresis. J Clin Microbiol 41:2498–2502 [View Article][PubMed]
    [Google Scholar]
  18. Vela A. I., Sánchez V., Mentaberre G., Lavín S., Domínguez L., Fernández-Garayzábal J. F. 2011a; Streptococcus porcorum sp. nov., isolated from domestic and wild pigs. Int J Syst Evol Microbiol 61:1585–1589 [View Article][PubMed]
    [Google Scholar]
  19. Vela A. I., Mentaberre G., Marco I., Velarde R., Lavín S., Domínguez L., Fernández-Garayzábal J. F. 2011b; Streptococcus rupicaprae sp. nov., isolated from a Pyrenean chamois (Rupicapra pyrenaica). Int J Syst Evol Microbiol 61:1989–1993 [View Article][PubMed]
    [Google Scholar]
  20. Whiley R. A., Hardie J. M. 2009; Genus I. Streptococcus Rosenbach 1884, 22AL . In Bergey's Manual of Systematic Bacteriology vol 3, 2nd edn. pp 655–711Edited by de Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. New York: Springer;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000697
Loading
/content/journal/ijsem/10.1099/ijsem.0.000697
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed