1887

Abstract

A Gram-stain-negative, rod-shaped, aerobic and non-motile strain, designated 4-T-34, was isolated from a till sample of Collins icecap front, Antarctica, and its taxonomic position was investigated by genotypic, phenotypic and chemotaxonomic analysis. The isolate grew at 4–30 °C (optimum 20–25 °C), at pH 6.0–10.0 and with 0–1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4-T-34 belonged to the genus , with the closest relatives being WT-MW11 (96.9 % 16S rRNA gene sequence similarity), ZS3-33 (96.8 %), IAM 12616 (96.5 %) and HDW-19 (95.4 %). Strain 4-T-34 contained Q-10 as the only ubiquinone and Cω7 as the major fatty acid. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminophospholipids and two unidentified phospholipids. The DNA G+C content of strain 4-T-34 was 61 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain 4-T-34 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 4-T-34 ( = CCTCC AB 2014005 = LMG 28256).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000693
2016-01-01
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/178.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000693&mimeType=html&fmt=ahah

References

  1. Bauer A. W. , Kirby W. M. , Sherris J. C. , Turck M. . ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496 [PubMed].
    [Google Scholar]
  2. Bowman J. P. . ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50: 1861–1868 [CrossRef] [PubMed].
    [Google Scholar]
  3. Chen C.-X. , Zhang X.-Y. , Liu C. , Yu Y. , Liu A. , Li G.-W. , Li H. , Chen X.-L. , Chen B. , other authors . ( 2013;). Pseudorhodobacter antarcticus sp nov., isolated from Antarctic intertidal sandy sediment, and emended description of the genus Pseudorhodobacter Uchino et al. 2002 emend. Jung et al. 2012. Int J Syst Evol Microbiol 63: 849–854 [CrossRef].
    [Google Scholar]
  4. Cowan S. T. . ( 1965;). Development of coding schemes for microbial taxonomy. Adv Appl Microbiol 7: 139–167 [CrossRef] [PubMed].
    [Google Scholar]
  5. Doetsch R. N. . ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. H. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  6. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  7. Jung Y.-T. , Oh K.-H. , Oh T.-K. , Yoon J.-H. . ( 2012;). Pseudorhodobacter aquimaris sp. nov., isolated from seawater, and emended description of the genus Pseudorhodobacter Uchino et al. 2002. Int J Syst Evol Microbiol 62: 100–105 [CrossRef] [PubMed].
    [Google Scholar]
  8. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kovacs N. . ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178: 703 [CrossRef] [PubMed].
    [Google Scholar]
  10. Lee M. H. , Lee S. Y. , Jung Y. T. , Park S. , Yoon J. H. . ( 2013;). Pseudorhodobacter wandonensis sp. nov., isolated from wood falls, and emended description of the genus Pseudorhodobacter . Int J Syst Evol Microbiol 63: 1479–1484 [CrossRef] [PubMed].
    [Google Scholar]
  11. Martens T. , Heidorn T. , Pukall R. , Simon M. , Tindall B. J. , Brinkhoff T. . ( 2006;). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . Int J Syst Evol Microbiol 56: 1293–1304 [CrossRef] [PubMed].
    [Google Scholar]
  12. Mesbah M. , Whitman W. B. . ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479: 297–306 [CrossRef] [PubMed].
    [Google Scholar]
  13. Reichenbach H. . ( 1992;). The order Cytophagales . . In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . New York: Springer; [CrossRef].
    [Google Scholar]
  14. Rüger H. J. , Höfle M. G. . ( 1992;). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42: 133–143 [CrossRef] [PubMed].
    [Google Scholar]
  15. Rzhetsky A. , Nei M. . ( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35: 367–375 [CrossRef] [PubMed].
    [Google Scholar]
  16. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  17. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  18. Stackebrandt E. , Goebel B. M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849.[CrossRef]
    [Google Scholar]
  19. Tamura K. , Nei M. . ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10: 512–526 [PubMed].
    [Google Scholar]
  20. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  21. Tindall B. J. . ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  22. Tresner H. D. , Danga F. . ( 1958;). Hydrogen sulfide production by Streptomyces as a criterion for species differentiation. J Bacteriol 76: 239–244 [PubMed].
    [Google Scholar]
  23. Uchino Y. , Hamada T. , Yokota A. . ( 2002;). Proposal of Pseudorhodobacter ferrugineus gen nov, comb nov, for a non-photosynthetic marine bacterium, Agrobacterium ferrugineum, related to the genus Rhodobacter . J Gen Appl Microbiol 48: 309–319 [CrossRef] [PubMed].
    [Google Scholar]
  24. Wilson K. H. , Blitchington R. B. , Greene R. C. . ( 1990;). Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction. J Clin Microbiol 28: 1942–1946 [PubMed].
    [Google Scholar]
  25. Xie C. H. , Yokota A. . ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49: 345–349 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000693
Loading
/content/journal/ijsem/10.1099/ijsem.0.000693
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error