1887

Abstract

A novel rod-shaped, Gram-stain-negative, non-gliding and aerobic strain surrounded by a multilayer capsule, designated 4-T-2, was isolated from a till sample of Collins glacier front, Antarctica. The bacterium formed yellow, circular, convex and smooth colonies. Growth occurred at 4–28 °C (optimum18–20 °C), at pH 7.0–10.0 (optimum pH 9.0) and with 0–1 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 4-T-2 belonged to the genus . Strain 4-T-2 shared the highest 16S rRNA gene sequence similarity with the type strain of (96.7 %). The DNA G+C content of strain 4-T-2 was 36.2 mol%. The only menaquinone was MK-6. The major cellular fatty acids were iso-C, iso-C G, iso-C and summed feature 9 (comprising iso-Cω9 and/or 10-methyl C). Polar lipid profile consisted phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and one unidentified lipid. On the basis of phylogenetic, physiological and chemotaxonomic data, strain 4-T-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 4-T-2 ( = CCTCC AB 2014004 = LMG 28257).

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2016-01-01
2019-10-21
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References

  1. Bauer A. W. , Kirby W. M. , Sherris J. C. , Turck M. . ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496 [PubMed].
    [Google Scholar]
  2. Bergey D. H. , Harrison F. C. , Breed R. S. , Hammer B. W. , Huntoon F. M. . (editors) ( 1923;). Genus II. Flavobacterium gen. nov. . In Bergey's Manual of Determinative Bacteriology, pp. 97–117 Baltimore: Williams & Wilkins;.
    [Google Scholar]
  3. Bernardet J. F. , Bowman J. P. . ( 2006;). The genus Flavobacterium . . In The Prokaryotes: a Handbook on the Biology of Bacteria, vol. 7, , 3rd edn.., pp. 481–531. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . New York:: [CrossRef] Springer;.
    [Google Scholar]
  4. Bernardet J. F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996;). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46: 128–148.[CrossRef]
    [Google Scholar]
  5. Bowman J. P. . ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50: 1861–1868 [CrossRef] [PubMed].
    [Google Scholar]
  6. Busse H.-J. , Auling G. . ( 1988;). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11: 1–8 [CrossRef].
    [Google Scholar]
  7. Busse H.-J. , Bunka S. , Hensel A. , Lubitz W. . ( 1997;). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47: 698–708 [CrossRef].
    [Google Scholar]
  8. Cowan S. T. . ( 1965;). Development of coding schemes for microbial taxonomy. Adv Appl Microbiol 7: 139–167 [CrossRef] [PubMed].
    [Google Scholar]
  9. Doetsch R. N. . ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by Gerhardt P. , Murray R. G. E. , Costilow R. N. , Nester E. W. , Wood W. A. , Krieg N. R. , Phillips G. H. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  10. Dong K. , Chen F. , Du Y. , Wang G. . ( 2013;). Flavobacterium enshiense sp. nov., isolated from soil, and emended descriptions of the genus Flavobacterium and Flavobacterium cauense, Flavobacterium saliperosum and Flavobacterium suncheonense . Int J Syst Evol Microbiol 63: 886–892 [CrossRef] [PubMed].
    [Google Scholar]
  11. Felsenstein J. . ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  12. Felsenstein J. . ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  13. Fitch W. M. . ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20: 406–416 [CrossRef].
    [Google Scholar]
  14. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kim J. H. , Choi B. H. , Jo M. , Kim S. C. , Lee P. C. . ( 2014;). Flavobacterium faecale sp. nov., an agarase-producing species isolated from stools of Antarctic penguins. Int J Syst Evol Microbiol 64: 2884–2890 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kovacs N. . ( 1956;). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178: 703 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kuo I. , Saw J. , Kapan D. D. , Christensen S. , Kaneshiro K. Y. , Donachie S. P. . ( 2013;). Flavobacterium akiainvivens sp. nov., from decaying wood of Wikstroemia oahuensis, Hawai'i, and emended description of the genus Flavobacterium . Int J Syst Evol Microbiol 63: 3280–3286 [CrossRef] [PubMed].
    [Google Scholar]
  18. McCammon S. A. , Bowman J. P. . ( 2000;). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50: 1055–1063 [CrossRef] [PubMed].
    [Google Scholar]
  19. McCammon S. A. , Innes B. H. , Bowman J. P. , Franzmann P. D. , Dobson S. J. , Holloway P. E. , Skerratt J. H. , Nichols P. D. , Rankin L. M. . ( 1998;). Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake. Int J Syst Bacteriol 48: 1405–1412 [CrossRef] [PubMed].
    [Google Scholar]
  20. Mesbah M. , Whitman W. B. . ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479: 297–306 [CrossRef] [PubMed].
    [Google Scholar]
  21. Miyashita M. , Fujimura S. , Nakagawa Y. , Nishizawa M. , Tomizuka N. , Nakagawa T. , Nakagawa J. . ( 2010;). Flavobacterium algicola sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 60: 344–348 [CrossRef] [PubMed].
    [Google Scholar]
  22. Murray R. G. E. , Doetsch R. N. , Robinow C. F. . ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  23. Reichenbach H. . ( 1992;). The order Cytophagales . . In The Prokaryotes, 4, , 2nd edn.., pp. 3631–3675. Edited by Balows A. , Trüper G. , Dworkin H. M. , Harder W. , Schleifer K. H. . New York:: [CrossRef] Springer;.
    [Google Scholar]
  24. Saitou N. , Nei M. . ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  25. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  26. Stackebrandt E. , Goebel B. M. . ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  27. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  28. Tindall B. J. . ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  29. Tresner H. D. , Danga F. . ( 1958;). Hydrogen sulfide production by Streptomyces as a criterion for species differentiation. J Bacteriol 76: 239–244 [PubMed].
    [Google Scholar]
  30. Van Trappen S. , Mergaert J. , Swings J. . ( 2003;). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53: 1241–1245 [CrossRef] [PubMed].
    [Google Scholar]
  31. Van Trappen S. , Vandecandelaere I. , Mergaert J. , Swings J. . ( 2004;). Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54: 85–92 [CrossRef] [PubMed].
    [Google Scholar]
  32. Van Trappen S. , Vandecandelaere I. , Mergaert J. , Swings J. . ( 2005;). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55: 769–772 [CrossRef] [PubMed].
    [Google Scholar]
  33. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173: 697–703 [PubMed].
    [Google Scholar]
  34. Xie C. H. , Yokota A. . ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49: 345–349 [CrossRef] [PubMed].
    [Google Scholar]
  35. Xin Y. H. , Liang Z. H. , Zhang D. C. , Liu H. C. , Zhang J. L. , Yu Y. , Xu M. S. , Zhou P. J. , Zhou Y. G. . ( 2009;). Flavobacterium tiangeerense sp. nov., a cold-living bacterium isolated from a glacier. Int J Syst Evol Microbiol 59: 2773–2777 [CrossRef] [PubMed].
    [Google Scholar]
  36. Yi H. , Chun J. . ( 2006;). Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. Int J Syst Evol Microbiol 56: 1239–1244 [CrossRef] [PubMed].
    [Google Scholar]
  37. Yi H. , Oh H. M. , Lee J. H. , Kim S. J. , Chun J. . ( 2005;). Flavobacterium antarcticum sp. nov., a novel psychrotolerant bacterium isolated from the Antarctic. Int J Syst Evol Microbiol 55: 637–641 [CrossRef] [PubMed].
    [Google Scholar]
  38. Zhu L. , Liu Q. , Liu H. , Zhang J. , Dong X. , Zhou Y. , Xin Y. . ( 2013;). Flavobacterium noncentrifugens sp. nov., a psychrotolerant bacterium isolated from glacier meltwater. Int J Syst Evol Microbiol 63: 2032–2037 [CrossRef] [PubMed].
    [Google Scholar]
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