1887

Abstract

Two Gram-stain-negative, catalase- and oxidase-positive, strictly aerobic, non-motile, moderately halophilic bacteria (strains X15M-6 and X15M-8) were isolated from Lake Xiaochaidan, a salt lake in Qaidam basin, Qinghai Province, China. Cells of X15M-6 were rod-like or coccoid, 0.5–0.9 μm wide and 0.9–1.5 μm long; cells of X15M-8 were rods, 0.3–0.6 μm wide and 1.2–2.2 μm long. Growth was observed in the presence of 0.5–14.0 % (w/v) NaCl (optimum, 3.0 %) and at pH 6.5–10.0 (optimum, pH 7.0–7.5) for both. X15M-6 and X15M-8 grew at 10–35 °C (optimum, 20–25 °C) and 4–35 °C (optimum, 25 °C), respectively. Both contained iso-C, anteiso-C and iso-C 3-OH as the major fatty acids, phosphatidylethanolamine and an unknown lipid as the major polar lipids, and menaquinone MK-6 as the major respiratory quinone. The DNA G+C contents were 32.8 and 35.0 mol% for X15M-6 and X15M-8, respectively. Phylogenetic trees based on 16S rRNA gene sequences showed that both strains belonged to the genus and formed a separate lineage. In addition, strains X15M-6 and X15M-8 shared 96.8 % 16S rRNA gene sequence similarity and showed highest similarities to members of the genus (92.7–93.5 and 91.8–93.1 %, respectively). Based on the above data, it is concluded that strains X15M-6 and X15M-8 represent two novel species of the genus , for which the names sp. nov. (type strain X15M-6 = CGMCC 1.12925 = JCM 30615) and sp. nov. (type strain X15M-8 = CGMCC 1.12931 = JCM 30616) are proposed.

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2016-01-01
2019-10-16
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