1887

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated HJM-2, was isolated from the place where the ocean and a freshwater lake meet at Hwajinpo, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HJM-2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain HJM-2 belonged to the genus , clustering coherently with the type strain of . Strain HJM-2 exhibited 16S rRNA gene sequence similarities of 98.6 and 97.2 % to CC-RR-82 and BS-R1, respectively, and 92.7–96.7 % to the type strains of the other species of the genus . Strain HJM-2 contained MK-7 as the predominant menaquinone and iso-C, summed feature 3 (Cω7 and/or Cω6) and iso-C 3-OH as the major fatty acids. The major polar lipids detected in strain HJM-2 were phosphatidylcholine, phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain HJM-2 was 45 mol% and mean DNA–DNA relatedness values with the type strains of and were 10–19 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain HJM-2 is separated from recognized species of the genus . On the basis of the data presented, strain HJM-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HJM-2 ( = KCTC 42704 = NBRC 111222).

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2016-01-01
2019-12-06
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