1887

Abstract

A novel bacterial strain, designated S5, was isolated from Pingaleshwar beach, in India. Cells were Gram-stain-positive, rod-shaped, non-motile and non-endospore-forming. Based on 16S rRNA gene sequence analysis, the strain was identified as belonging to the class and was related most closely to DSM 13869 (97.6 % sequence similarity). However, it shared only 93.1 % 16S rRNA gene sequence similarity with NBRC 15112, the type species of the genus, indicating that strain S5 might not be a member of the genus . The DNA–DNA relatedness between strain S5 and DSM 13869 was 39 %. The cell-wall peptidoglycan contained -diaminopimelic acid. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol and two phospholipids. Isoprenoid quinones were absent from strain S5. Fatty acid analysis revealed that anteiso-C, C and iso-C were the predominant fatty acids present. The results of phylogenetic, chemotaxonomic and biochemical tests allowed the clear differentiation of strain S5, which is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is S5 ( = KCTC 33632 = CGMCC 1.15177). Based on the present study, it is also suggested to transfer to this new genus, as comb. nov. The type strain of is Z-7984 = (DSM 13869 = UNIQEM 210).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000678
2016-01-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/1/84.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000678&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Bagheri M., Didari M., Shahzedeh Fazeli S. A., Schumann P., Sánchez-Porro C., Ventosa A.. ( 2013;). Saliterribacillus persicus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a hypersaline lake. Int J Syst Evol Microbiol 63: 345–351 [CrossRef] [PubMed].
    [Google Scholar]
  2. Amoozegar M. A., Bagheri M., Didari M., Mehrshad M., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A.. ( 2014;). Aquibacillus halophilus gen. nov., sp. nov., a moderately halophilic bacterium from a hypersaline lake, and reclassification of Virgibacillus koreensis as Aquibacillus koreensis comb. nov. and Virgibacillus albus as Aquibacillus albus comb. nov. Int J Syst Evol Microbiol 64: 3616–3623 [CrossRef] [PubMed].
    [Google Scholar]
  3. An S. Y., Ishikawa S., Kasai H., Goto K., Yokota A.. ( 2007;). Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan. Int J Syst Evol Microbiol 57: 2489–2492 [CrossRef] [PubMed].
    [Google Scholar]
  4. Arahal D. R., Ventosa A.. ( 2002;). Moderately halophilic and halotolerant species of Bacillus and related genera. . In Applications and Systematics of Bacillus and Relatives, pp. 83–99. Edited by Berkeley R., Heyndrickx M., Logan N., De Vos P.. Oxford:: [CrossRef] Blackwell;.
    [Google Scholar]
  5. Cao S. J., Qu J. H., Yang J. S., Sun Q., Yuan H. L.. ( 2008;). Halolactibacillus alkaliphilus sp. nov., a moderately alkaliphilic and halophilic bacterium isolated from a soda lake in Inner Mongolia, China, and emended description of the genus Halolactibacillus. Int J Syst Evol Microbiol 58: 2169–2173 [CrossRef] [PubMed].
    [Google Scholar]
  6. Chen Y.-G., Cui X.-L., Zhang Y.-Q., Li W.-J., Wang Y.-X., Xu L.-H., Wen M.-L., Peng Q., Jiang C.-L.. ( 2009;). Paraliobacillus quinghaiensis sp. nov., isolated from salt-lake sediment in China. Int J Syst Evol Microbiol 59: 28–33 [CrossRef] [PubMed].
    [Google Scholar]
  7. de la Haba R. R., Sánchez-Porro C., Márquez M. C., Ventosa A.. ( 2011;). Taxonomy of halophiles. . In Extremophiles Handbook, pp. 255–308. Edited by Horikoshi K.. Tokyo:: [CrossRef] Springer;.
    [Google Scholar]
  8. Hasegawa T., Takizaea M., Tanida S.. ( 1983;). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29: 319–322 [CrossRef].
    [Google Scholar]
  9. Hirota K., Aino K., Nodasaka Y., Morita N., Yumoto I.. ( 2013;). Amphibacillus indicireducens sp. nov., an alkaliphile that reduces an indigo dye. Int J Syst Evol Microbiol 63: 464–469 [CrossRef] [PubMed].
    [Google Scholar]
  10. Hirota K., Hanaoka Y., Nodasaka Y., Yumoto I.. ( 2014;). Gracilibacillus alcaliphilus sp. nov., a facultative alkaliphile isolated from indigo fermentation liquor for dyeing. Int J Syst Evol Microbiol 64: 3174–3180 [CrossRef] [PubMed].
    [Google Scholar]
  11. Horikoshi K..), ( 2011;). Extremophiles Handbook Heidelberg:: [CrossRef] Springer;.
    [Google Scholar]
  12. Ishikawa M., Ishizaki S., Yamamoto Y., Yamasato K.. ( 2002;). Paraliobacillus ryukyuensis gen. nov., sp. nov., a new Gram-positive, slightly halophilic, extremely halotolerant, facultative anaerobe isolated from a decomposing marine alga. J Gen Appl Microbiol 48: 269–279 [CrossRef] [PubMed].
    [Google Scholar]
  13. Ishikawa M., Nakajima K., Itamiya Y., Furukawa S., Yamamoto Y., Yamasato K.. ( 2005;). Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. Int J Syst Evol Microbiol 55: 2427–2439 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kates M.. ( 1972;). Techniques of Lipidology New York:: [CrossRef] Elsevier;.
    [Google Scholar]
  15. Kates M.. ( 1986;). Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids Amsterdam: Elsevier;.
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  17. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  18. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59: 2114–2121 [CrossRef] [PubMed].
    [Google Scholar]
  19. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208–218 [CrossRef].
    [Google Scholar]
  20. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H.-P.. ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195: 413–418 [CrossRef] [PubMed].
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  22. Niimura Y., Suzuki K.-I.. ( 2009;). Family I. Bacillaceae. . In Bergey's Manual of Systematic Bacteriology, 2nd edn., pp. 139. Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B.. Dordrect: Springer;.
    [Google Scholar]
  23. Niimura Y., Koh E., Yanagida F., Suzuki K.-I., Komagata K., Kozaki M.. ( 1990;). Amphibacillus xylanus gen. nov., sp. nov., a facultatively anaerobic spore forming xylan-digesting bacterium which lacks cytochrome, quinone, and catalase. Int J Syst Bacteriol 40: 297–301 [CrossRef].
    [Google Scholar]
  24. Oren A., Duker S., Ritter S.. ( 1996;). The polar lipid composition of Walsby's square bacterium. FEMS Microbiol Lett 138: 135–140 [CrossRef].
    [Google Scholar]
  25. Reddy S. V., Aspana S., Tushar D. L., Sasikala Ch., Ramana ChV.. ( 2013;). Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar. Int J Syst Evol Microbiol 63: 2223–2228 [CrossRef] [PubMed].
    [Google Scholar]
  26. Ren B., Yang N., Wang J., Ma X. L., Wang Q., Xie F., Guo H., Liu Z. H., Pugin B., Zhang L. X.. ( 2013;). Amphibacillus marinus sp. nov., a member of the genus Amphibacillus isolated from marine mud. Int J Syst Evol Microbiol 63: 1485–1491 [CrossRef] [PubMed].
    [Google Scholar]
  27. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  28. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18: 123–156 [CrossRef].
    [Google Scholar]
  29. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407–477 [PubMed].
    [Google Scholar]
  30. Seiler H., Wenning M., Scherer S.. ( 2013;). Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room. Int J Syst Evol Microbiol 63: 2054–2061 [CrossRef] [PubMed].
    [Google Scholar]
  31. Smibert R. M., Krieg N. R.. ( 1981;). General characterization. . In Manual of Methods for General Microbiology, pp. 409–443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  32. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  33. Stackebrandt E., Ebers J.. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 4: 152–155.
    [Google Scholar]
  34. Stackebrandt E., Goebel B. M.. ( 1994;). Place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  35. Tamaoka J., Fujimura Y.-K., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol 54: 31–36.
    [Google Scholar]
  36. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  37. Tindall B. J.. ( 1990a;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  38. Tindall B. J.. ( 1990b;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13: 128–130 [CrossRef].
    [Google Scholar]
  39. Tourova T. P., Antonov A. S.. ( 1988;). Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19: 333–355 [CrossRef].
    [Google Scholar]
  40. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A.. ( 1982;). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128: 1959–1968.
    [Google Scholar]
  41. Wainø M., Tindall B. J., Schumann P., Ingvorsen K.. ( 1999;). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49: 821–831 [CrossRef] [PubMed].
    [Google Scholar]
  42. Wang X., Xue Y., Ma Y.. ( 2011;). Streptohalobacillus salinus gen. nov., sp. nov., a moderately halophilic, Gram-positive, facultative anaerobe isolated from subsurface saline soil. Int J Syst Evol Microbiol 61: 1127–1132 [CrossRef] [PubMed].
    [Google Scholar]
  43. Wu X. Y., Zheng G., Zhang W. W., Xu X. W., Wu M., Zhu X. F.. ( 2010;). Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake. Int J Syst Evol Microbiol 60: 2540–2543 [CrossRef] [PubMed].
    [Google Scholar]
  44. Yang G., Zhou S.. ( 2014;). Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae. Int J Syst Evol Microbiol 64: 1647–1653 [CrossRef] [PubMed].
    [Google Scholar]
  45. Zhilina T. N., Garnova E. S., Turova T. P., Kostrikina N. A., Zavarzin G. A.. ( 2001;). [Amphibacillus fermentum sp. nov., Amphibacillus tropicus sp. nov. – new alkaliphilic, facultatively anaerobic, saccharolytic Bacilli from Lake Magadi]. Mikrobiologiia 70: 825–837 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000678
Loading
/content/journal/ijsem/10.1099/ijsem.0.000678
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error