1887

Abstract

A novel actinobacterium, designated strain NEAU-QY2, was isolated from the leaves of L. specimen, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism formed single spores with rough surfaces on substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-QY2 belonged to the genus and formed a monophyletic clade with its closest related strains YIM 68255 (99.2 % 16S rRNA gene sequence similarity), NEAU-FHS4 (98.8 %) and YIM 61359 (98.7 %), an association that was supported by a bootstrap value of 90 % in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, DNA–DNA hybridization values between strain NEAU-QY2 and the three closely related strains were below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, strain NEAU-QY2 was distinguished from closely related strains and is classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-QY2 ( = CGMCC 4.7216 = JCM 30345).

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2015-12-01
2019-10-19
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