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Abstract

A yellow-pigmented bacterial strain, designated Y2, was isolated from farmland soil in Bengbu, Anhui province, China. Cells of strain Y2 were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Strain Y2 grew optimally at pH 7.0, 30 °C and in the presence of 2 % (w/v) NaCl. The DNA G+C content was 68.9 mol%. The major fatty acids (>5 %) were iso-C, iso-C, summed feature 9 (C 10-methyl and/or iso-Cω9), iso-C 3-OH and iso-C. The major respiratory quinone was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain Y2 was most closely related to B1953/27.1 (99.1 % 16S rRNA gene sequence similarity), followed by G3 (98.6 %), XH031 (96.2 %) and RIB1-20 (96.0 %). Strain Y2 exhibited low DNA–DNA relatedness with B1953/27.1 (43.6 ± 0.5 %) and G3 (43.9 ± 2.1 %). On the basis of phenotypic, genotypic and phylogenetic evidence, strain Y2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y2 ( = ACCC 19799 = KCTC 42441).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31370155, J1210056 and J1310015)
  • he Project for Science and Technology of Jiangsu Province (Award BE2012749)
  • the Project for Science and Technology of Guangdong Province (Award 2013B090500017)
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2015-12-01
2021-10-20
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. ), ( 1995). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology , 3rd edn. New York: Wiley;.
    [Google Scholar]
  2. Baik K. S., Park S. C., Kim M. S., Kim E. M., Park C., Chun J., Seong C. N. ( 2008;). Luteimonas marina sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58 29042908 [View Article] [PubMed].
    [Google Scholar]
  3. Beveridge T. J., Lawrence J. R., Murray R. G. E. ( 2007;). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, pp. 1933. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder R. L. , 3rd edn.., Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  4. Breznak J. A., Costilow R. N. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  5. Chou J. H., Cho N. T., Arun A. B., Young C. C., Chen W. M. ( 2008;). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. Int J Syst Evol Microbiol 58 20512055 [View Article] [PubMed].
    [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221230 [View Article] [PubMed].
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. ( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12 133142 [View Article] [PubMed].
    [Google Scholar]
  8. Dong X. Z., Cai M. Y. ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press;.
    [Google Scholar]
  9. Ebersole L. L. ( 1992;). Acid-fast stain procedures. . In Clinical Microbiology Procedures Handbook, pp. 3.5.13.5.11. Edited by Isenberg H. D. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  10. Fan X., Yu T., Li Z., Zhang X. H. ( 2014;). Luteimonas abyssi sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 64 668674 [View Article] [PubMed].
    [Google Scholar]
  11. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  12. Finkmann W., Altendorf K., Stackebrandt E., Lipski A. ( 2000;). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50 273282 [View Article] [PubMed].
    [Google Scholar]
  13. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  15. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed].
    [Google Scholar]
  16. Lane D. J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E. R., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  18. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O. ( 2011;). Luteimonas lutimaris sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 61 27292733 [View Article] [PubMed].
    [Google Scholar]
  19. Roh S. W., Kim K. H., Nam Y. D., Chang H. W., Kim M. S., Yoon J. H., Oh H. M., Bae J. W. ( 2008;). Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 46 525529 [View Article] [PubMed].
    [Google Scholar]
  20. Romanenko L. A., Tanaka N., Svetashev V. I., Kurilenko V. V., Mikhailov V. V. ( 2013;). Luteimonas vadosa sp. nov., isolated from seashore sediment. Int J Syst Evol Microbiol 63 12611266 [View Article] [PubMed].
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  22. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20 16.
    [Google Scholar]
  23. Stackebrandt E., Goebel B. M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  24. Sun Z. B., Zhang H., Yuan X. F., Wang Y. X., Feng D. M., Wang Y. H., Feng Y. J. ( 2012;). Luteimonas cucumeris sp. nov., isolated a from cucumber leaf. Int J Syst Evol Microbiol 62 29162920 [View Article] [PubMed].
    [Google Scholar]
  25. Sun L. N., Zhang J., Gong F. F., Wang X., Hu G., Li S. P., Hong Q. ( 2014;). Nocardioides soli sp. nov., a carbendazim-degrading bacterium isolated from soil under the long-term application of carbendazim. Int J Syst Evol Microbiol 64 20472052 [View Article] [PubMed].
    [Google Scholar]
  26. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 2001;). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51 16391652 [View Article] [PubMed].
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  29. Wu G., Liu Y., Li Q., Du H., You J., Li H., Ke C., Zhang X., Yu J., Zhao T. ( 2013;). Luteimonas huabeiensis sp. nov., isolated from stratum water. Int J Syst Evol Microbiol 63 33523357 [View Article] [PubMed].
    [Google Scholar]
  30. Young C. C., Kämpfer P., Chen W. M., Yen W. S., Arun A. B., Lai W. A., Shen F. T., Rekha P. D., Lin K.-Y., Chou J.-H. ( 2007;). Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. Int J Syst Evol Microbiol 57 741744 [View Article] [PubMed].
    [Google Scholar]
  31. Zhang D. C., Liu H. C., Xin Y. H., Zhou Y. G., Schinner F., Margesin R. ( 2010;). Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. Int J Syst Evol Microbiol 60 15811584 [View Article] [PubMed].
    [Google Scholar]
  32. Zhang H., Cheng M. G., Sun B., Guo S. H., Song M., Li Q., Huang X. ( 2015;). Flavobacterium suzhouense sp. nov., isolated from farmland river sludge. Int J Syst Evol Microbiol 65 370374 [View Article] [PubMed].
    [Google Scholar]
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