1887

Abstract

Two novel species, spec. nov. and spec. nov., isolated from coastal waters of Hangzhou Bay, China, were investigated with standard methods. is characterized as follows: size 95–135 × 50–85 μm; usually one spherical macronucleus; 12–15 prolonged caudal cilia; two or three preoral kineties and 42–50 somatic kineties; membranelle 1 (M1) about 20 % of the anterior fragment of membranelle 2 (M2a) in length, consisting of three longitudinal rows of kinetosomes; posterior end of M2a hook-like; membranelle 3 (M3) three-rowed. is characterized as follows: size about 55–85 × 25–55 μm; four or five preoral kineties and 21–23 somatic kineties; posterior end of M2a hook-like; M3 three-rowed. The small-subunit rRNA gene was sequenced for both species. Phylogenetic analyses revealed that is most closely related to and that is sister to and (GenBank accession no. JX310015). With the inclusion of the two new sequences, the monophyly of the genus is not supported.

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2015-12-01
2019-09-22
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References

  1. Agatha S., Spindler M., Wilbert N.. ( 1993;). Ciliated protozoa (Ciliophora) from Arctic sea ice. Acta Protozool 32: 261–268.
    [Google Scholar]
  2. Corliss J. O., Snyder R. A.. ( 1986;). A preliminary description of several new ciliates from the Antarctica, including Cohnilembus grassei n. sp. and Pleuronema tardum n. sp. (Hymenostomata, Pleuronematina). Protistologica (Paris) 22: 39–46.
    [Google Scholar]
  3. de Castro L. A. M., Küppers G. C., Schweikert M., Harada M. L., Paiva T. S.. ( 2014;). Ciliates from eutrophized water in the northern Brazil and morphology of Cristigera hammeri Wilbert, 1986 (Ciliophora, Scuticociliatia). Eur J Protistol 50: 122–133 [CrossRef] [PubMed].
    [Google Scholar]
  4. Dragesco J.. ( 1968;). Les genres Pleuronema Dujardin, Schizocalyptra nov. gen. et Histiobalantium Stokes (ciliés holotriches hyménostomes). Protistologica (Paris) 4: 85–106 (in French).
    [Google Scholar]
  5. Dragesco J., Dragesco-Kernéis A.. ( 1986;). Ciliés Libres de l'Afrique Intertropicale (Collection Faune Tropicale, vol. 26) Paris: Editions de l'ORSTROM (in French);.
    [Google Scholar]
  6. Fan X., Miao M., Al-Rasheid K. A. S., Song W.. ( 2009;). A new genus of marine scuticociliate (Protozoa, Ciliophora) from northern China, with a brief note on its phylogenetic position inferred from small subunit ribosomal DNA sequence data. J Eukaryot Microbiol 56: 577–582 [CrossRef] [PubMed].
    [Google Scholar]
  7. Fan X., Hu X., Al-Farraj S. A., Clamp J. C., Song W.. ( 2011a;). Morphological description of three marine ciliates (Ciliophora, Scuticociliatia), with establishment of a new genus and two new species. Eur J Protistol 47: 186–196 [CrossRef] [PubMed].
    [Google Scholar]
  8. Fan X., Lin X., Al-Rasheid K. A. S., Al-Farraj S. A., Warren A., Song W.. ( 2011b;). The diversity of scuticociliates (Protozoa, Ciliophora): a report on eight marine forms found in coastal waters of China, with a description of one new species. Acta Protozool 50: 219–234.
    [Google Scholar]
  9. Fan X., Al-Farraj S. A., Gao F., Gu F.. ( 2014;). Morphological reports on two species of Dexiotricha (Ciliophora, Scuticociliatia), with a note on the phylogenetic position of the genus. Int J Syst Evol Microbiol 64: 680–688 [CrossRef] [PubMed].
    [Google Scholar]
  10. Fernandez-Leborans G., Novillo A.. ( 1994;). Morphology and taxonomic position of two marine pleuronematine species: Pleuronema lynni and Schizocalyptra marina (Protozoa, Ciliophora). J Zool (Lond) 233: 259–275 [CrossRef].
    [Google Scholar]
  11. Foissner W., Jung J. H., Filker S., Rudolph J., Stoeck T.. ( 2014;). Morphology, ontogenesis and molecular phylogeny of Platynematum salinarum nov. spec., a new scuticociliate (Ciliophora, Scuticociliatia) from a solar saltern. Eur J Protistol 50: 174–184 [CrossRef] [PubMed].
    [Google Scholar]
  12. Gao F., Katz L. A.. ( 2014;). Phylogenomic analyses support the bifurcation of ciliates into two major clades that differ in properties of nuclear division. Mol Phylogenet Evol 70: 240–243 [CrossRef] [PubMed].
    [Google Scholar]
  13. Gao F., Katz L. A., Song W.. ( 2013;). Multigene-based analyses on evolutionary phylogeny of two controversial ciliate orders: Pleuronematida and Loxocephalida (Protista, Ciliophora, Oligohymenophorea). Mol Phylogenet Evol 68: 55–63 [CrossRef] [PubMed].
    [Google Scholar]
  14. Gao F., Gao S., Wang P., Katz L. A., Song W.. ( 2014;). Phylogenetic analyses of cyclidiids (Protista, Ciliophora, Scuticociliatia) based on multiple genes suggest their close relationship with thigmotrichids. Mol Phylogenet Evol 75: 219–226 [CrossRef] [PubMed].
    [Google Scholar]
  15. Long H., Song W., Warren A., Al-Rasheid K., Gong J., Chen X.. ( 2007;). Two new ciliates from the north China seas, Schizocalyptra aeschtae nov. spec. and Sathrophilus holtae nov. spec., with new definition of the genus Sathrophilus (Ciliophora, Oligohymenophora). Acta Protozool 46: 229–245.
    [Google Scholar]
  16. Lynn D. H.. ( 2008;). The Ciliated Protozoa, Characterization, Classification, and Guide to the Literature, 3rd edn. Dordrecht: Springer;.
    [Google Scholar]
  17. Medlin L., Elwood H. J., Stickel S., Sogin M. L.. ( 1988;). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491–499 [CrossRef] [PubMed].
    [Google Scholar]
  18. Miao M., Wang Y., Song W., Clamp J. C., Al-Rasheid K. A. S.. ( 2010;). Description of Eurystomatella sinica n. gen., n. sp., with establishment of a new family Eurystomatellidae n. fam. (Protista, Ciliophora, Scuticociliatia) and analyses of its phylogeny inferred from sequences of the small-subunit rRNA gene. Int J Syst Evol Microbiol 60: 460–468 [CrossRef] [PubMed].
    [Google Scholar]
  19. Nylander J. A.. ( 2004;). MrModeltest version 2 Uppsala University;.
    [Google Scholar]
  20. Pan H., Huang J., Hu X., Fan X., Al-Rasheid K. A. S., Song W.. ( 2010;). Morphology and SSU rRNA gene sequences of three marine ciliates from Yellow Sea, China, including one new species, Uronema heteromarinum nov. spec. (Ciliophora, Scuticociliatida). Acta Protozool 49: 45–59.
    [Google Scholar]
  21. Pan X., Shao C., Ma H., Fan X., Al-Rasheid K. A. S., Al-Farraj S. A., Hu X.. ( 2011;). Redescriptions of two marine scuticociliates from China, with notes on stomatogenesis in Parauronema longum (Ciliophora, Scuticociliatida). Acta Protozool 50: 301–310.
    [Google Scholar]
  22. Pan X., Huang J., Fan X., Ma H., Al-Rasheid K. A. S., Miao M., Gao F.. ( 2015;). Morphology and phylogeny of four marine scuticociliates (Protista, Ciliophora), with descriptions of two new species: Pleuronema elegans spec. nov. and Uronema orientalis spec. nov. Acta Protozool 54: 31–43.
    [Google Scholar]
  23. Penn O., Privman E., Landan G., Graur D., Pupko T.. ( 2010a;). An alignment confidence score capturing robustness to guide tree uncertainty. Mol Biol Evol 27: 1759–1767 [CrossRef] [PubMed].
    [Google Scholar]
  24. Penn O., Privman E., Ashkenazy H., Landan G., Graur D., Pupko T.. ( 2010b;). guidance: a web server for assessing alignment confidence scores. Nucleic Acids Res 38: W23–W28 [CrossRef] [PubMed].
    [Google Scholar]
  25. Posada D., Crandall K. A.. ( 1998;). modeltest: testing the model of DNA substitution. Bioinformatics 14: 817–818 [CrossRef] [PubMed].
    [Google Scholar]
  26. Ronquist F., Huelsenbeck J. P.. ( 2003;). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574 [CrossRef] [PubMed].
    [Google Scholar]
  27. Song W.. ( 2000;). Morphology and taxonomical studies on some marine scuticociliates from China sea, with description of two new species, Philasterides armatalis sp. n. and Cyclidium varibonneti sp. n. (Protozoa: Ciliophora: Scuticociliatida). Acta Protozool 39: 295–322.
    [Google Scholar]
  28. Song W., Warren A., Hu X.. ( 2009;). Free-living Ciliates in the Bohai and Yellow Sea, China Beijing: Science Press;.
    [Google Scholar]
  29. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690 [CrossRef] [PubMed].
    [Google Scholar]
  30. Stamatakis A., Hoover P., Rougemont J.. ( 2008;). A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol 57: 758–771 [CrossRef] [PubMed].
    [Google Scholar]
  31. Wang Y., Song W., Hu X., Warren A., Chen X., Al-Rasheid K. A. S.. ( 2008a;). Descriptions of two new marine species of Pleuronema, P. czapikae sp. n. and P. wiackowskii sp. n. (Ciliophora: Scuticociliatida), from the Yellow Sea, north China. Acta Protozool 47: 35–45.
    [Google Scholar]
  32. Wang Y., Miao M., Zhang Q., Gao S., Song W., Al-Rasheid K. A. S., Warren A., Ma H.. ( 2008b;). Three marine interstitial scuticociliates, Schizocalyptra similis sp. n., S. sinica sp. n. and Hippocomos salinus Small and Lynn, 1985 (Ciliophora: Scuticociliatida), isolated from Chinese coastal waters. Acta Protozool 47: 377–387.
    [Google Scholar]
  33. Wang Y., Hu X., Long H., Al-Rasheid K. A., Al-Farraj S. A., Song W.. ( 2008c;). Morphological studies indicate that Pleuronema grolierei nov. spec. and P. coronatum Kent, 1881 represent different sections of the genus Pleuronema (Ciliophora: Scuticociliatida). Eur J Protistol 44: 131–140 [CrossRef] [PubMed].
    [Google Scholar]
  34. Wang Y., Song W., Warren A., Al-Rasheid K. A. S., Al-Quraishy S. A., Al-Farraj S. A., Hu X., Pan H.. ( 2009;). Descriptions of two new marine scuticociliates, Pleuronema sinica n. sp. and P. wilberti n. sp. (Ciliophora: Scuticociliatida), from the Yellow Sea, China. Eur J Protistol 45: 29–37 [CrossRef] [PubMed].
    [Google Scholar]
  35. Wilbert N.. ( 1975;). Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos 64: 171–179 (in German).
    [Google Scholar]
  36. Yi Z., Wang Y., Lin X., Al-Rasheid K. A. S., Song W.. ( 2010;). Phylogeny of subclass Scuticociliatia (Protozoa, Ciliophora) using combined data inferred from genetic, morphological, and morphogenetic evidence. Chin J Oceanol Limnol 28: 778–784 [CrossRef].
    [Google Scholar]
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