1887

Abstract

A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103, was isolated from saltern soil in Gomso, Korea. Cells of strain BH103 were strictly aerobic, motile, straight rods and grew at pH 7.0–10.8 (optimum, pH 8.5), at 10–55 °C (optimum, 28 °C) and at salinities of 0–23 % (w/v) NaCl (optimum, 14 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103 belongs to the genus , showing highest sequence similarity to LC1 (97.7 %), Eplume1 (97.7 %), III (97.7 %), CRSS (97.7 %), TYRC17 (97.5 %), BH1 (97.2 %) and Esulfide1 (96.2 %). The predominant ubiquinone was Q-9. The major fatty acids were Cω7, Cω7 and/or iso-C 2-OH, C and C 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA–DNA relatedness between strain BH103 and KACC 16615, KCTC 2888, KCTC 2889, KCTC 22844, DSM 19074, JCM 16411 and DSM 15722 was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BH103 ( = KACC 17609 = NBRC 109914 = NCAIM B 02528).

Funding
This study was supported by the:
  • , Rural Development Administration, Republic of Korea
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2015-12-01
2021-02-27
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