1887

Abstract

subsp. subsp. nov. and subsp. subsp. nov. are described. These emanate from the three known phylotypes of , designated types I, II and III. Electron microscopy confirmed the filamentous cell shape of type III, showing a striking difference from types I/II, which were short rods. Biochemical tests indicated that, in types I/II, either the pyruvate, -pyrrolidonyl arylamidase or -ribose 2 test was positive, whereas all of these were negative among type III strains. Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) spectra, which profile mainly their ribosomal proteins, were different between these two groups. Surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) spectra of all phylotypes revealed a specific protein biomarker that was overexpressed in type III strains compared with types I/II only when grown aerobically. Reference strains had high whole-genome similarity between types I (>91 %) and II (>75 %), but a considerably lower level of 72 % similarity with type III. and sequence dendrograms confirmed the distant relatedness of type III, indicating the presence of two distinct centres of variation within the species . On the other hand, cellular fatty acid profiles and 16S rRNA gene sequence relatedness (>99.3 %) circumscribed the species. Thus, we propose two subspecies, subsp. subsp. nov. for types I/II and subsp. subsp. nov. for type III. The type strain of subsp. is NCTC 737 ( = ATCC 6919 = JCM 6425 = DSM 1897 = CCUG 1794), while the type strain of subsp. is K124 ( = NCTC 13655 = JCM 18919).

Funding
This study was supported by the:
  • Institution for Fermentation, Osaka (IFO), Japan (Award 18790808)
  • Japan Society for the Promotion of Science
  • British Council Japan Scholarship
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2015-12-01
2021-10-19
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