A novel actinomycete, designated strain NEAU-A-2T, was isolated from the rhizosphere soil of Sansevieria trifasciata Prain collected from Heilongjiang province, north-east China. The taxonomic status of this organism was established using a polyphasic approach. The isolate formed irregular sporangia containing motile spores on the substrate mycelium. The whole-cell sugars were xylose and galactose. The predominant menaquinones were MK-9(H10), MK-9(H2), MK-10(H2) and MK-10(H4). The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and an unidentified glycolipid. 16S rRNA gene sequence similarity studies showed that strain NEAU-A-2T belongs to the genus Actinoplanes with the highest sequence similarities to Actinoplanes globisporus NBRC 13912T (97.7 % 16S rRNA gene sequence similarity), Actinoplanes ferrugineus IMSNU 22125T (97.5 %), Actinoplanes toevensis MN07-A0368T (97.2 %) and Actinoplanes rishiriensis NBRC 108556T (97.2 %); similarities to type strains of other species of this genus were < 97 %. Two tree-making algorithms showed that strain NEAU-A-2T formed a distinct clade with A. globisporus NBRC 13912T and A. rishiriensis NBRC 108556T. However, low DNA–DNA relatedness values allowed the isolate to be differentiated from the above-mentioned two species of the genus Actinoplanes. Moreover, strain NEAU-A-2T could also be distinguished from the most closely related species by morphological and physiological characteristics. Therefore, in conclusion, isolate NEAU-A-2T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes rhizophilus sp. nov. is proposed. The type strain is NEAU-A-2T ( = CGMCC 4.7133T = DSM 46672T).
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Funding
This study was supported by the:
the National Outstanding Youth Foundation
(Award 31225024)
CollinsM. D.
( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by
GoodfellowM.,
MinnikinD. E.
London:
Academic Press;.
De LeyJ.,
CattoirH.,
ReynaertsA.
( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12133–142 [View Article][PubMed]
.
GaoR.,
LiuC.,
ZhaoJ.,
JiaF.,
YuC.,
YangL.,
WangX.,
XiangW.
( 2014b;). Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora
. Antonie van Leeuwenhoek105307–315 [View Article][PubMed]
.
GoodfellowM.,
StantonL. J.,
SimpsonK. E.,
MinnikinD. E.
( 1990;). Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol13619–36 [View Article].
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC.H.-N.
( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol2454–63 [View Article].
HayakawaM.,
NonomuraH.
( 1987;). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol65501–509 [View Article].
HussV.A.R.,
FestlH.,
SchleiferK. H.
( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4184–192 [View Article][PubMed]
.
KellyK. L.
( 1964). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors
Washington, DC:
US Government Printing Office;.
KimuraM.
( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16111–120 [View Article][PubMed]
.
LechevalierM. P.,
LechevalierH. A.
( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication, no. 6), pp. 227–291. Edited by
DietzA.,
ThayerD. W.
Arlington, VA:
Society for Industrial Microbiology;.
MandelM.,
MarmurJ.
( 1968;). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B195–206 [View Article].
McKerrowJ.,
VaggS.,
McKinneyT.,
SeviourE. M.,
MaszenanA. M.,
BrooksP.,
SeviourR. J.
( 2000;). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol30178–182 [View Article][PubMed]
.
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.
( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2233–241 [View Article].
SmibertR. M.,
KriegN. R.
( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC:
American Society for Microbiology;.
TamuraT.,
HatanoK.
( 2001;). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol512119–2125 [View Article][PubMed]
.
UchidaK.,
KudoT.,
SuzukiK. I.,
NakaseT.
( 1999;). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol4549–56 [View Article][PubMed]
.
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP.A.D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW.E.C.,
MurrayR.G.E.,
other authors.
( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37463–464 [View Article].
WinkJ.,
SchumannP.,
SpöerC.,
EisenbarthK.,
GlaeserS. P.,
MartinK.,
KämpferP.
( 2014;). Emended description of Actinoplanes friuliensis and description of Actinoplanes nipponensis sp. nov., antibiotic-producing species of the genus Actinoplanes
. Int J Syst Evol Microbiol64599–606 [View Article][PubMed]
.
XiangW.,
LiuC.,
WangX.,
DuJ.,
XiL.,
HuangY.
( 2011;). Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol611165–1169 [View Article][PubMed]
.
YokotaA.,
TamuraT.,
HasegawaT.,
HuangL. H.
( 1993;). Catenuloplanes japonicas gen. nov., sp. nov., nom. rev., a new genus of the order Actinomycetales
. Int J Syst Bacteriol43805–812 [View Article].