1887

Abstract

A Gram-stain-variable, rod-shaped, non-motile and endospore-forming bacterium, designated strain HZ1, was isolated from a sample of bank side soil from Hangzhou city, Zhejiang province, PR China. On the basis of 16S rRNA gene sequence analysis, strain HZ1 was closely related to members of the genus sharing the highest levels of sequence similarity with DSM 1327 (94.4 %), KIT00200-70066-1 (94.4 %). Growth occurred at 15–42 °C (optimum 30–37 °C), pH 5.0–9.5 (optimum pH 7.0–8.0) and NaCl concentrations of up to 6.0 % (w/v) were tolerated (optimum 0.5 %). The dominant respiratory quinone was MK-7 and the DNA G+C content was 40.1 mol%. The major fatty acids were anteiso-C and iso-C. The major polar lipids of strain HZ1 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and several unknown lipids. The diagnostic diamino acid found in the cell-wall peptidoglycan was -diaminopimelic acid. Based on its phenotypic and chemotaxonomic characteristics and phylogenetic data, strain HZ1 represents a novel species of the genus , for which the name sp. nov. (type strain HZ1 = CCTCC AB 2014276 = LMG 28639) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000645
2015-12-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4757.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000645&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins D. C.. ( 1993;). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253–260 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bae J. Y., Kim K. Y., Kim J. H., Lee K., Cho J. C., Cha C. J.. ( 2010;). Paenibacillus aestuarii sp. nov., isolated from an estuarine wetland. Int J Syst Evol Microbiol 60: 644–647 [CrossRef] [PubMed].
    [Google Scholar]
  3. Baker G. C., Smith J. J., Cowan D. A.. ( 2003;). Review and re-analysis of 257 domain-specific 16S primers. J Microbiol Methods 55: 541–555 [CrossRef] [PubMed].
    [Google Scholar]
  4. Carro L., Flores-Félix J. D., Cerda-Castillo E., Ramírez-Bahena M. H., Igual J. M., Tejedor C., Velázquez E., Peix A.. ( 2013;). Paenibacillus endophyticus sp. nov., isolated from nodules of Cicer arietinum. Int J Syst Evol Microbiol 63: 4433–4438 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycan based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48: 459–470 [CrossRef].
    [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  7. Dong X., Cai M.. ( 2001;). Manual of Systematic and Determinative Bacteriology Beijing, China: Academic Press;.
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  9. Jeon C. O., Lim J. M., Lee S. S., Chung B. S., Park D. J., Xu L. H., Jiang C. L., Kim C. J.. ( 2009;). Paenibacillus harenae sp. nov., isolated from desert sand in China. Int J Syst Evol Microbiol 59: 13–17 [CrossRef] [PubMed].
    [Google Scholar]
  10. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N.. [CrossRef] Academic Press;.
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M.. ( 1996;). Numerical analysis offatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42: 989–1005 [CrossRef].
    [Google Scholar]
  12. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H.-J., Tindall B. J.. ( 2006;). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Int J Syst Evol Microbiol 56: 781–786 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kämpfer P., Falsen E., Lodders N., Martin K., Kassmannhuber J., Busse H. J.. ( 2012;). Paenibacillus chartarius sp. nov., isolated from a paper mill. Int J Syst Evol Microbiol 62: 1342–1347 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kim K. K., Lee K. C., Yu H., Ryoo S., Park Y., Lee J. S.. ( 2010;). Paenibacillus sputi sp. nov., isolated from the sputum of a patient with pulmonary disease. Int J Syst Evol Microbiol 60: 2371–2376 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  16. Kittiwongwattana C., Thawai C.. ( 2015;). Paenibacillus lemnae sp. nov., an endophytic bacterium of duckweed (Lemna aequinoctialis). Int J Syst Evol Microbiol 65: 107–112 [CrossRef] [PubMed].
    [Google Scholar]
  17. Li J., Lu Q., Liu T., Zhou S., Yang G., Zhao Y.. ( 2014;). Paenibacillus guangzhouensis sp. nov., an Fe (III)- and humus-reducing bacterium from a forest soil. Int J Syst Evol Microbiol 64: 3891–3896 [CrossRef] [PubMed].
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise Measurement of the G+C Content of Deoxyribonucleic-Acid by High-Performance Liquid-Chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  19. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47: 87–95 [CrossRef].
    [Google Scholar]
  20. Montes M. J., Mercadé E., Bozal N., Guinea J.. ( 2004;). Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54: 1521–1526 [CrossRef] [PubMed].
    [Google Scholar]
  21. Nakagawa Y., Yamasato K.. ( 1993;). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139: 1155–1161 [CrossRef] [PubMed].
    [Google Scholar]
  22. Rzhetsky A., Nei M.. ( 1992;). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9: 945–967.
    [Google Scholar]
  23. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  24. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  25. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18: 123–156 [CrossRef].
    [Google Scholar]
  26. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K.. ( 1997;). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47: 289–298 [CrossRef] [PubMed].
    [Google Scholar]
  27. Šmerda J., Sedlácek I., Pácová Z., Durnová E., Smísková A., Havel L.. ( 2005;). Paenibacillus mendelii sp. nov., from surface-sterilized seeds of Pisum sativum L. Int J Syst Evol Microbiol 55: 2351–2354 [CrossRef] [PubMed].
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  29. Tang Q. Y., Yang N., Wang J., Xie Y. Q., Ren B., Zhou Y. G., Gu M. Y., Mao J., Li W. J., other authors. ( 2011;). Paenibacillus algorifonticola sp. nov., isolated from a cold spring. Int J Syst Evol Microbiol 61: 2167–2172 [CrossRef] [PubMed].
    [Google Scholar]
  30. Uetanabaro A. P., Wahrenburg C., Hunger W., Pukall R., Spröer C., Stackebrandt E., de Canhos V. P., Claus D., Fritze D., other authors. ( 2003;). Paenibacillus agarexedens sp. nov., nom. rev., and Paenibacillus agaridevorans sp. nov. Int J Syst Evol Microbiol 53: 1051–1057 [CrossRef] [PubMed].
    [Google Scholar]
  31. Wang L., Baek S. H., Cui Y., Lee H. G., Lee S. T.. ( 2012;). Paenibacillus sediminis sp. nov., a xylanolytic bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 62: 1284–1288 [CrossRef] [PubMed].
    [Google Scholar]
  32. Zhang L., Wang Y., Dai J., Tang Y., Yang Q., Luo X., Fang C.. ( 2009;). Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China. Int J Syst Evol Microbiol 59: 1787–1792 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000645
Loading
/content/journal/ijsem/10.1099/ijsem.0.000645
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error