sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to Free

Abstract

The name ‘’ dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with but clearly differed from for the milky white aspect of the colonies. The sequence similarity with the type strain of ranged, in eight additionally investigated genetic targets, from 78.9 to 94.3 %, an evident contrast with the close relatedness that emerged at the level of 16S rRNA gene. The average nucleotide identity between the genomes of DSM 44166 and strain 126/5/03 (type strain of the novel species) was 92.92 %, and supported the status of independent species. The confirmation of the name sp. nov. is proposed, with strain 126/5/03 ( = CIP 109313 = DSM 45057) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000642
2015-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4724.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000642&mimeType=html&fmt=ahah

References

  1. Adékambi T., Colson P., Drancourt M. ( 2003;). rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol 41 56995708 [View Article] [PubMed] .
    [Google Scholar]
  2. CDC ( 1996). Standardized Method for HPLC Identification of Mycobacteria Atlanta, GA: Public Health Service US Department of Health and Human Services;.
    [Google Scholar]
  3. Christensen H., Bisgaard M., Frederiksen W., Mutters R., Kuhnert P., Olsen J. E. ( 2001;). Is characterization of a single isolate sufficient for valid publication of a new genus or species? Proposal to modify recommendation 30b of the Bacteriological Code (1990 Revision). Int J Syst Evol Microbiol 51 22212225 [View Article] [PubMed] .
    [Google Scholar]
  4. CLSI ( 2011). Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes Approved Standard, 2nd edn, M24-A2 Wayne, PA: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  5. Dai J., Chen Y., Dean S., Morris J. G., Salfinger M., Johnson J. A. ( 2011;). Multiple-genome comparison reveals new loci for Mycobacterium species identification. J Clin Microbiol 49 144153 [PubMed]. [CrossRef]
    [Google Scholar]
  6. Davies E., Wieboldt J., Stanley T., Maeda Y., Smyth M., Stanley S., McClean M., Evans W., Funston C., other authors. ( 2012;). Isolation and identification of ‘Mycobacterium angelicum’ from a patient with type II respiratory failure: suggested reporting guidelines to molecular clinical laboratories. Br J Biomed Sci 69 134136 [PubMed].
    [Google Scholar]
  7. Durnez L., Suykerbuyk P., Nicolas V., Barrière P., Verheyen E., Johnson C. R., Leirs H., Portaels F. ( 2010;). Terrestrial small mammals as reservoirs of Mycobacterium ulcerans in Benin. Appl Environ Microbiol 76 45744577 [View Article] [PubMed] .
    [Google Scholar]
  8. Gauthier D. T., Rhodes M. W. ( 2009;). Mycobacteriosis in fishes: a review. Vet J 180 3347 [View Article] [PubMed] .
    [Google Scholar]
  9. Gomila M., Ramirez A., Lalucat J. ( 2007;). Diversity of environmental Mycobacterium isolates from hemodialysis water as shown by a multigene sequencing approach. Appl Environ Microbiol 73 37873797 [View Article] [PubMed] .
    [Google Scholar]
  10. Kent P. T., Kubica G. P. ( 1985). Public Health Mycobacteriology. A Guide for the Level III Laboratory Atlanta, GA: US Department of Health and Human Services;.
    [Google Scholar]
  11. Kim M., Oh H. S., Park S. C., Chun J. ( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64 346351 [View Article] [PubMed] .
    [Google Scholar]
  12. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C. ( 1993;). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol 31 28822889 [PubMed].
    [Google Scholar]
  13. Konstantinidis K. T., Tiedje J. M. ( 2005;). Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci U S A 102 25672572 [View Article] [PubMed] .
    [Google Scholar]
  14. Levi M. H., Bartell J., Gandolfo L., Smole S. C., Costa S. F., Weiss L. M., Johnson L. K., Osterhout G., Herbst L. H. ( 2003;). Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex . J Clin Microbiol 41 21472152 [View Article] [PubMed] .
    [Google Scholar]
  15. Marumo K., Nakamura H., Tazawa S., Kazumi Y., Kawano R., Shirata C., Taguchi K., Kikuchi T., Nagashima G. ( 2010;). Isolation of novel mycobacteria contaminating an aquarium fish tank in a Japanese university hospital. J Appl Microbiol 109 558566 [PubMed].
    [Google Scholar]
  16. McNabb A., Eisler D., Adie K., Amos M., Rodrigues M., Stephens G., Black W. A., Isaac-Renton J. ( 2004;). Assessment of partial sequencing of the 65-kiloDalton heat shock protein gene (hsp65) for routine identification of mycobacterium species isolated from clinical sources. J Clin Microbiol 42 30003011 [View Article] [PubMed] .
    [Google Scholar]
  17. Pourahmad F., Cervellione F., Thompson K. D., Taggart J. B., Adams A., Richards R. H. ( 2008;). Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish. Int J Syst Evol Microbiol 58 28212827 [PubMed]. [CrossRef]
    [Google Scholar]
  18. Rhodes M. W., Kator H., Kotob S., van Berkum P., Kaattari I., Vogelbein W., Quinn F., Floyd M. M., Butler W. R., Ottinger C. A. ( 2003;). Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol 53 421424 [View Article] [PubMed] .
    [Google Scholar]
  19. Rhodes M. W., Kator H., McNabb A., Deshayes C., Reyrat J. M., Brown-Elliott B. A., Wallace R. Jr, Trott K. A., Parker J. M., other authors. ( 2005;). Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol 55 11391147 [View Article] [PubMed] .
    [Google Scholar]
  20. Roth A., Fischer M., Hamid M. E., Michalke S., Ludwig W., Mauch H. ( 1998;). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36 139147 [PubMed].
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  22. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed] .
    [Google Scholar]
  23. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. ( 1993;). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31 175178 [PubMed].
    [Google Scholar]
  24. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 46734680 [View Article] [PubMed] .
    [Google Scholar]
  25. Tortoli E., Pecorari M., Fabio G., Messinò M., Fabio A. ( 2010;). Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species. J Clin Microbiol 48 307310 [View Article] [PubMed] .
    [Google Scholar]
  26. Whipps C. M., Butler W. R., Pourahmad F., Watral V. G., Kent M. L. ( 2007;). Molecular systematics support the revival of Mycobacterium salmoniphilum (ex Ross 1960) sp. nov., nom. rev., a species closely related to Mycobacterium chelonae . Int J Syst Evol Microbiol 57 25252531 [View Article] [PubMed] .
    [Google Scholar]
  27. Zerbino D. R., Birney E. ( 2008;). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18 821829 [View Article] [PubMed] .
    [Google Scholar]
  28. Zolg J. W., Philippi-Schulz S. ( 1994;). The superoxide dismutase gene, a target for detection and identification of mycobacteria by PCR. J Clin Microbiol 32 28012812 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000642
Loading
/content/journal/ijsem/10.1099/ijsem.0.000642
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed