The name ‘Mycobacterium angelicum’ dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with Mycobacterium szulgai but clearly differed from M. szulgai for the milky white aspect of the colonies. The sequence similarity with the type strain of M. szulgai ranged, in eight additionally investigated genetic targets, from 78.9 to 94.3 %, an evident contrast with the close relatedness that emerged at the level of 16S rRNA gene. The average nucleotide identity between the genomes of M. szulgai DSM 44166T and strain 126/5/03T (type strain of the novel species) was 92.92 %, and supported the status of independent species. The confirmation of the name Mycobacterium angelicum sp. nov. is proposed, with strain 126/5/03T ( = CIP 109313T = DSM 45057T) as the type strain.
CDC ( 1996). Standardized Method for HPLC Identification of Mycobacteria
Atlanta, GA:
Public Health Service
US Department of Health and Human Services;.
ChristensenH.,
BisgaardM.,
FrederiksenW.,
MuttersR.,
KuhnertP.,
OlsenJ. E.
( 2001;). Is characterization of a single isolate sufficient for valid publication of a new genus or species? Proposal to modify recommendation 30b of the Bacteriological Code (1990 Revision). Int J Syst Evol Microbiol512221–2225 [View Article][PubMed]
.
DaiJ.,
ChenY.,
DeanS.,
MorrisJ. G.,
SalfingerM.,
JohnsonJ. A.
( 2011;). Multiple-genome comparison reveals new loci for Mycobacterium species identification. J Clin Microbiol49144–153[PubMed]. [CrossRef]
DaviesE.,
WieboldtJ.,
StanleyT.,
MaedaY.,
SmythM.,
StanleyS.,
McCleanM.,
EvansW.,
FunstonC.,
other authors.
( 2012;). Isolation and identification of ‘Mycobacterium angelicum’ from a patient with type II respiratory failure: suggested reporting guidelines to molecular clinical laboratories. Br J Biomed Sci69134–136[PubMed].
DurnezL.,
SuykerbuykP.,
NicolasV.,
BarrièreP.,
VerheyenE.,
JohnsonC. R.,
LeirsH.,
PortaelsF.
( 2010;). Terrestrial small mammals as reservoirs of Mycobacterium ulcerans in Benin. Appl Environ Microbiol764574–4577 [View Article][PubMed]
.
GomilaM.,
RamirezA.,
LalucatJ.
( 2007;). Diversity of environmental Mycobacterium isolates from hemodialysis water as shown by a multigene sequencing approach. Appl Environ Microbiol733787–3797 [View Article][PubMed]
.
KentP. T.,
KubicaG. P.
( 1985). Public Health Mycobacteriology. A Guide for the Level III Laboratory
Atlanta, GA:
US Department of Health and Human Services;.
KimM.,
OhH. S.,
ParkS. C.,
ChunJ.
( 2014;). Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol64346–351 [View Article][PubMed]
.
KirschnerP.,
SpringerB.,
VogelU.,
MeierA.,
WredeA.,
KiekenbeckM.,
BangeF. C.,
BöttgerE. C.
( 1993;). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol312882–2889[PubMed].
KonstantinidisK. T.,
TiedjeJ. M.
( 2005;). Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci U S A1022567–2572 [View Article][PubMed]
.
LeviM. H.,
BartellJ.,
GandolfoL.,
SmoleS. C.,
CostaS. F.,
WeissL. M.,
JohnsonL. K.,
OsterhoutG.,
HerbstL. H.
( 2003;). Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex
. J Clin Microbiol412147–2152 [View Article][PubMed]
.
MarumoK.,
NakamuraH.,
TazawaS.,
KazumiY.,
KawanoR.,
ShirataC.,
TaguchiK.,
KikuchiT.,
NagashimaG.
( 2010;). Isolation of novel mycobacteria contaminating an aquarium fish tank in a Japanese university hospital. J Appl Microbiol109558–566[PubMed].
McNabbA.,
EislerD.,
AdieK.,
AmosM.,
RodriguesM.,
StephensG.,
BlackW. A.,
Isaac-RentonJ.
( 2004;). Assessment of partial sequencing of the 65-kiloDalton heat shock protein gene (hsp65) for routine identification of mycobacterium species isolated from clinical sources. J Clin Microbiol423000–3011 [View Article][PubMed]
.
PourahmadF.,
CervellioneF.,
ThompsonK. D.,
TaggartJ. B.,
AdamsA.,
RichardsR. H.
( 2008;). Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish. Int J Syst Evol Microbiol582821–2827[PubMed]. [CrossRef]
RhodesM. W.,
KatorH.,
KotobS.,
van BerkumP.,
KaattariI.,
VogelbeinW.,
QuinnF.,
FloydM. M.,
ButlerW. R.,
OttingerC. A.
( 2003;). Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol53421–424 [View Article][PubMed]
.
RhodesM. W.,
KatorH.,
McNabbA.,
DeshayesC.,
ReyratJ. M.,
Brown-ElliottB. A.,
WallaceR.Jr,
TrottK. A.,
ParkerJ. M.,
other authors.
( 2005;). Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol551139–1147 [View Article][PubMed]
.
TelentiA.,
MarchesiF.,
BalzM.,
BallyF.,
BöttgerE. C.,
BodmerT.
( 1993;). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol31175–178[PubMed].
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.
( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res224673–4680 [View Article][PubMed]
.
TortoliE.,
PecorariM.,
FabioG.,
MessinòM.,
FabioA.
( 2010;). Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species. J Clin Microbiol48307–310 [View Article][PubMed]
.
WhippsC. M.,
ButlerW. R.,
PourahmadF.,
WatralV. G.,
KentM. L.
( 2007;). Molecular systematics support the revival of Mycobacterium salmoniphilum (ex Ross 1960) sp. nov., nom. rev., a species closely related to Mycobacterium chelonae
. Int J Syst Evol Microbiol572525–2531 [View Article][PubMed]
.
ZerbinoD. R.,
BirneyE.
( 2008;). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res18821–829 [View Article][PubMed]
.
ZolgJ. W.,
Philippi-SchulzS.
( 1994;). The superoxide dismutase gene, a target for detection and identification of mycobacteria by PCR. J Clin Microbiol322801–2812[PubMed].