1887

Abstract

Seven strains, ICMP 19430, ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10–37 °C (optimum, 25–30 °C), at pH 4.0–9.0 (optimum, pH 6.0–7.0) and with 0–2 % (w/v) NaCl (optimum growth in the absence of NaCl). On the basis of 16S rRNA gene sequence analysis, the strains showed 99.0–99.5 % sequence similarity to the closest type strain, PsJN, and 98.4–99.7 % sequence similarity to LMG 19076. The predominant fatty acids were Cω7 (21.0 % of the total fatty acids in strain ICMP 19430), C (19.1 %), C cyclo (18.9 %), summed feature 3 (Cω7 and/or Cω6; 10.7 %) and C cyclo ω8 (7.5 %). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was Q-8 and the DNA G+C content of strain ICMP 19430 was 63.2 mol%. The DNA–DNA relatedness of the novel strains with respect to the closest neighbouring members of the genus was 55 % or less. On the basis of 16S rRNA and gene sequence similarities and chemotaxonomic and phenotypic data, these strains represent a novel symbiotic species in the genus , for which the name sp. nov. is proposed, with the type strain ICMP 19430 ( = LMG 28415 = HAMBI 3637).

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2015-12-01
2020-09-30
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