1887

Abstract

Strain TCF032-E4 was isolated from a traditional Chinese fermented radish. It shares >99% 16S rRNA sequence identity with and . This strain can ferment ribose, galactose, glucose, fructose, mannose, mannitol, -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, melibiose, trehalose and gentiobiose. It cannot ferment sucrose, which can be used by , and as well as most of the strains (88.7%). TCF032-E4 cannot grow at temperature above 32 °C. This strain shares 78.2–83.6% (phenylalanyl-tRNA synthetase alpha subunit) and 89.5–94.9% (RNA polymerase alpha subunit) sequence identity with , and . These results indicate that TCF032-E4 represents a distinct species. This hypothesis was further confirmed by whole-genome sequencing and comparison with available genomes of related species. The draft genome size of TCF032-E4 is approximately 2.9 Mb, with a DNA G+C content of 43.5 mol%. The average nucleotide identity (ANI) between TCF032-E4 and related species ranges from 79.0 to 81.1%, the highest ANI value being observed with subsp ATCC 14917. A novel species, sp. nov., is proposed with TCF032-E4 ( = CCTCC AB2015090 = DSM 100358) as the type strain.

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2015-12-01
2019-10-22
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References

  1. Bringel F., Curk M. C., Hubert J. C.. ( 1996;). Characterization of lactobacilli by Southern-type hybridization with a Lactobacillus plantarum pyrDFE probe. Int J Syst Bacteriol 46: 588–594 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bringel F., Castioni A., Olukoya D. K., Felis G. E., Torriani S., Dellaglio F.. ( 2005;). Lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices. Int J Syst Evol Microbiol 55: 1629–1634 [CrossRef] [PubMed].
    [Google Scholar]
  3. Cappuccino J. G., Sherman N.. ( 2002;). Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin Cummings;.
    [Google Scholar]
  4. Curk M. C., Hubert J. C., Bringel F.. ( 1996;). Lactobacillus paraplantarum sp. now., a new species related to Lactobacillus plantarum. Int J Syst Bacteriol 46: 595–598 [CrossRef] [PubMed].
    [Google Scholar]
  5. Darling A. E., Mau B., Perna N. T.. ( 2010;). progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One 5: e11147 [CrossRef] [PubMed].
    [Google Scholar]
  6. De Bruyne K., Camu N., De Vuyst L., Vandamme P.. ( 2009;). Lactobacillus fabifermentans sp. nov. and Lactobacillus cacaonum sp. nov., isolated from Ghanaian cocoa fermentations. Int J Syst Evol Microbiol 59: 7–12 [CrossRef] [PubMed].
    [Google Scholar]
  7. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M.. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57: 81–91 [CrossRef] [PubMed].
    [Google Scholar]
  8. Gu C. T., Wang F., Li C. Y., Liu F., Huo G. C.. ( 2012;). Lactobacillus xiangfangensis sp. nov., isolated from Chinese pickle. Int J Syst Evol Microbiol 62: 860–863 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gu C. T., Li C. Y., Yang L. J., Huo G. C.. ( 2013;). Lactobacillus mudanjiangensis sp. nov., Lactobacillus songhuajiangensis sp. nov. and Lactobacillus nenjiangensis sp. nov., isolated from Chinese traditional pickle and sourdough. Int J Syst Evol Microbiol 63: 4698–4706 [CrossRef] [PubMed].
    [Google Scholar]
  10. Mao Y., Chen M., Horvath P.. ( 2015;). Draft genome sequence of Lactobacillus sp. strain TCF032-E4, isolated from fermented radish. Genome Announc 3: e00821–e00815 [CrossRef] [PubMed].
    [Google Scholar]
  11. Mattarelli P., Holzapfel W., Franz C.M.A.P., Endo A., Felis G. E., Hammes W., Pot B., Dicks L., Dellaglio F.. ( 2014;). Recommended minimal standards for description of new taxa of the genera Bifidobacterium, Lactobacillus and related genera. Int J Syst Evol Microbiol 64: 1434–1451 [CrossRef] [PubMed].
    [Google Scholar]
  12. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J.. ( 2007;). Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 57: 2777–2789 [CrossRef] [PubMed].
    [Google Scholar]
  13. Overbeek R., Olson R., Pusch G. D., Olsen G. J., Davis J. J., Disz T., Edwards R. A., Gerdes S., Parrello B., other authors. ( 2014;). The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). 42 (D1), D206–D214 [CrossRef] [PubMed].
    [Google Scholar]
  14. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36: 407–477 [PubMed].
    [Google Scholar]
  15. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28: 226–231 [PubMed].
    [Google Scholar]
  16. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  17. Torriani S., Felis G. E., Dellaglio F.. ( 2001;). Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Appl Environ Microbiol 67: 3450–3454 [CrossRef] [PubMed].
    [Google Scholar]
  18. Zanoni P., Farrow J. A. E., Phillips B. A., Collins M. D.. ( 1987;). Lactobacillus pentosus (Fred, Peterson, and Anderson) sp. nov., nom, rev. Int J Syst Bacteriol 37: 339–341 [CrossRef].
    [Google Scholar]
  19. Zerbino D. R., Birney E.. ( 2008;). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18: 821–829 [CrossRef] [PubMed].
    [Google Scholar]
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