1887

Abstract

Strain TCF032-E4 was isolated from a traditional Chinese fermented radish. It shares >99% 16S rRNA sequence identity with and . This strain can ferment ribose, galactose, glucose, fructose, mannose, mannitol, -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, melibiose, trehalose and gentiobiose. It cannot ferment sucrose, which can be used by , and as well as most of the strains (88.7%). TCF032-E4 cannot grow at temperature above 32 °C. This strain shares 78.2–83.6% (phenylalanyl-tRNA synthetase alpha subunit) and 89.5–94.9% (RNA polymerase alpha subunit) sequence identity with , and . These results indicate that TCF032-E4 represents a distinct species. This hypothesis was further confirmed by whole-genome sequencing and comparison with available genomes of related species. The draft genome size of TCF032-E4 is approximately 2.9 Mb, with a DNA G+C content of 43.5 mol%. The average nucleotide identity (ANI) between TCF032-E4 and related species ranges from 79.0 to 81.1%, the highest ANI value being observed with subsp ATCC 14917. A novel species, sp. nov., is proposed with TCF032-E4 ( = CCTCC AB2015090 = DSM 100358) as the type strain.

Funding
This study was supported by the:
  • DuPont Nutrition and Health
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000636
2015-12-01
2021-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4682.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000636&mimeType=html&fmt=ahah

References

  1. Bringel F., Curk M. C., Hubert J. C. ( 1996;). Characterization of lactobacilli by Southern-type hybridization with a Lactobacillus plantarum pyrDFE probe. Int J Syst Bacteriol 46 588594 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bringel F., Castioni A., Olukoya D. K., Felis G. E., Torriani S., Dellaglio F. ( 2005;). Lactobacillus plantarum subsp. argentoratensis subsp. nov., isolated from vegetable matrices. Int J Syst Evol Microbiol 55 16291634 [CrossRef] [PubMed].
    [Google Scholar]
  3. Cappuccino J. G., Sherman N. ( 2002). Microbiology: a Laboratory Manual , 6th edn. Menlo Park, CA: Benjamin Cummings;.
    [Google Scholar]
  4. Curk M. C., Hubert J. C., Bringel F. ( 1996;). Lactobacillus paraplantarum sp. now., a new species related to Lactobacillus plantarum . Int J Syst Bacteriol 46 595598 [CrossRef] [PubMed].
    [Google Scholar]
  5. Darling A. E., Mau B., Perna N. T. ( 2010;). progressiveMauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One 5 e11147 [CrossRef] [PubMed].
    [Google Scholar]
  6. De Bruyne K., Camu N., De Vuyst L., Vandamme P. ( 2009;). Lactobacillus fabifermentans sp. nov. and Lactobacillus cacaonum sp. nov., isolated from Ghanaian cocoa fermentations. Int J Syst Evol Microbiol 59 712 [CrossRef] [PubMed].
    [Google Scholar]
  7. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57 8191 [CrossRef] [PubMed].
    [Google Scholar]
  8. Gu C. T., Wang F., Li C. Y., Liu F., Huo G. C. ( 2012;). Lactobacillus xiangfangensis sp. nov., isolated from Chinese pickle. Int J Syst Evol Microbiol 62 860863 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gu C. T., Li C. Y., Yang L. J., Huo G. C. ( 2013;). Lactobacillus mudanjiangensis sp. nov., Lactobacillus songhuajiangensis sp. nov. and Lactobacillus nenjiangensis sp. nov., isolated from Chinese traditional pickle and sourdough. Int J Syst Evol Microbiol 63 46984706 [CrossRef] [PubMed].
    [Google Scholar]
  10. Mao Y., Chen M., Horvath P. ( 2015;). Draft genome sequence of Lactobacillus sp. strain TCF032-E4, isolated from fermented radish. Genome Announc 3 e00821e00815 [CrossRef] [PubMed].
    [Google Scholar]
  11. Mattarelli P., Holzapfel W., Franz C.M.A.P., Endo A., Felis G. E., Hammes W., Pot B., Dicks L., Dellaglio F. ( 2014;). Recommended minimal standards for description of new taxa of the genera Bifidobacterium, Lactobacillus and related genera. Int J Syst Evol Microbiol 64 14341451 [CrossRef] [PubMed].
    [Google Scholar]
  12. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J. ( 2007;). Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 57 27772789 [CrossRef] [PubMed].
    [Google Scholar]
  13. Overbeek R., Olson R., Pusch G. D., Olsen G. J., Davis J. J., Disz T., Edwards R. A., Gerdes S., Parrello B., other authors. ( 2014;). The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). 42 (D1), D206D214 [CrossRef] [PubMed].
    [Google Scholar]
  14. Schleifer K. H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  15. Staneck J. L., Roberts G. D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 226231 [PubMed].
    [Google Scholar]
  16. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [CrossRef] [PubMed].
    [Google Scholar]
  17. Torriani S., Felis G. E., Dellaglio F. ( 2001;). Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Appl Environ Microbiol 67 34503454 [CrossRef] [PubMed].
    [Google Scholar]
  18. Zanoni P., Farrow J. A. E., Phillips B. A., Collins M. D. ( 1987;). Lactobacillus pentosus (Fred, Peterson, and Anderson) sp. nov., nom, rev. Int J Syst Bacteriol 37 339341 [CrossRef].
    [Google Scholar]
  19. Zerbino D. R., Birney E. ( 2008;). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18 821829 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000636
Loading
/content/journal/ijsem/10.1099/ijsem.0.000636
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error