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Abstract

Strain TCF032-E4 was isolated from a traditional Chinese fermented radish. It shares >99% 16S rRNA sequence identity with and . This strain can ferment ribose, galactose, glucose, fructose, mannose, mannitol, -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, melibiose, trehalose and gentiobiose. It cannot ferment sucrose, which can be used by , and as well as most of the strains (88.7%). TCF032-E4 cannot grow at temperature above 32 °C. This strain shares 78.2–83.6% (phenylalanyl-tRNA synthetase alpha subunit) and 89.5–94.9% (RNA polymerase alpha subunit) sequence identity with , and . These results indicate that TCF032-E4 represents a distinct species. This hypothesis was further confirmed by whole-genome sequencing and comparison with available genomes of related species. The draft genome size of TCF032-E4 is approximately 2.9 Mb, with a DNA G+C content of 43.5 mol%. The average nucleotide identity (ANI) between TCF032-E4 and related species ranges from 79.0 to 81.1%, the highest ANI value being observed with subsp ATCC 14917. A novel species, sp. nov., is proposed with TCF032-E4 ( = CCTCC AB2015090 = DSM 100358) as the type strain.

Funding
This study was supported by the:
  • DuPont Nutrition and Health
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2015-12-01
2024-12-02
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