sp. nov., isolated from ark shell Free

Abstract

A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated MA4-5, was isolated from ark shell () collected from the South Sea, South Korea. The novel strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences showed that strain MA4-5 forms a coherent cluster with the type strains of , and , sharing 97.0–99.2 % sequence similarity. It exhibited 16S rRNA gene sequence similarity of 93.1–96.1 % to the type strains of other species. Strain MA4-5 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The major polar lipids of strain MA4-5 were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain MA4-5 was 53.8 mol% and its mean DNA–DNA relatedness values with the type strains of , and were 11–26 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, indicated that strain MA4-5 is separate from recognized species of the genus . On the basis of the data presented, strain MA4-5 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MA4-5 ( = KCTC 42703 = NBRC 111226).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000633
2015-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4695.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000633&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. (editors) ( 1993). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn.. Cambridge:: [View Article] Cambridge University Press;.
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Balows H. G. Schlegel. Berlin: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51 19972006 [View Article] [PubMed].
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49 2568 [View Article] [PubMed].
    [Google Scholar]
  5. Embley T. M., Wait R. ( 1994;). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121161. Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;.
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  7. González J. M., Covert J. S., Whitman W. B., Henriksen J. R., Mayer F., Scharf B., Schmitt R., Buchan A., Fuhrman J. A., other authors. ( 2003;). Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov. dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53 12611269 [View Article] [PubMed].
    [Google Scholar]
  8. Hameed A., Shahina M., Lin S. Y., Nakayan P., Liu Y.-C., Lai W.-A., Hsu Y.-H. ( 2014;). Youngimonas vesicularis gen. nov., sp. nov., of the family Rhodobacteraceae, isolated from surface seawater, reclassification of Donghicola xiamenensis Tan et al. 2009 as Pseudodonghicola xiamenensis gen. nov., comb. nov. and emended description of the genus Donghicola Yoon et al. 2007. Int J Syst Evol Microbiol 64 27292737 [View Article] [PubMed].
    [Google Scholar]
  9. Jung Y.-T., Park S., Yoon J.-H. ( 2012;). Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula. Antonie van Leeuwenhoek 102 141148 [View Article] [PubMed].
    [Google Scholar]
  10. Kim Y.-O., Kong H. J., Park S., Kang S.-J., Kim W.-J., Kim K.-K., Oh T.-K., Yoon J.-H. ( 2012;). Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi. Int J Syst Evol Microbiol 62 931936 [View Article] [PubMed].
    [Google Scholar]
  11. Kim Y.-O., Park S., Nam B.-H., Kim D.-G., Yoon J.-H. ( 2014;). Pseudopelagicola gijangensis gen. nov., sp. nov., isolated from the sea squirt Halocynthia roretzi . Int J Syst Evol Microbiol 64 34473452 [PubMed]. [CrossRef]
    [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [View Article].
    [Google Scholar]
  13. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. ( 1999;). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49 137147 [View Article] [PubMed].
    [Google Scholar]
  14. Lai Q., Zhong H., Wang J., Yuan J., Sun F., Wang L., Zheng T., Shao Z. ( 2011;). Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean. Int J Syst Evol Microbiol 61 20402044 [View Article] [PubMed].
    [Google Scholar]
  15. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 167 [View Article].
    [Google Scholar]
  16. Lucena T., Ruvira M. A., Macián M. C., Pujalte M. J., Arahal D. R. ( 2014;). Roseovarius albus sp. nov., a new Alphaproteobacterium isolated from the Mediterranean Sea. Antonie van Leeuwenhoek 105 671678 [View Article] [PubMed].
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  18. Park S., Kang C.-H., Park J.-M., Yoon J.-H. ( 2014a;). Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter . Antonie van Leeuwenhoek 106 647655 [View Article] [PubMed].
    [Google Scholar]
  19. Park S., Park D.-S., Bae K. S., Yoon J.-H. ( 2014b;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64 13781383 [View Article] [PubMed].
    [Google Scholar]
  20. Park S., Park J.-M., Kang C.-H., Yoon J.-H. ( 2014c;). Roseovarius gaetbuli sp. nov., a novel alphaproteobacterium isolated from a tidal flat sediment. Antonie van Leeuwenhoek 105 723730 [View Article] [PubMed].
    [Google Scholar]
  21. Park S., Park J.-M., Lee K.-C., Bae K. S., Yoon J.-H. ( 2014d;). Boseongicola aestuarii gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 64 26182624 [PubMed]. [CrossRef]
    [Google Scholar]
  22. Park S., Park J.-M., Kang C.-H., Yoon J.-H. ( 2015;). Aliiroseovarius pelagivivens gen. nov., sp. nov., isolated from seawater, and reclassification of three species of the genus Roseovarius as Aliiroseovarius crassostreae comb. nov., Aliiroseovarius halocynthiae comb. nov. and Aliiroseovarius sediminilitoris comb. nov. Int J Syst Evol Microbiol 65 26462652 [View Article] [PubMed].
    [Google Scholar]
  23. Parte A. C. ( 2014;). LPSN – list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42 (D1), D613D616 [View Article] [PubMed].
    [Google Scholar]
  24. Rajasabapathy R., Mohandass C., Dastager S. G., Liu Q., Khieu T.-N., Son C. K., Li W.-J., Colaco A. ( 2014;). Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores. Antonie van Leeuwenhoek 105 571578 [View Article] [PubMed].
    [Google Scholar]
  25. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846849 [View Article].
    [Google Scholar]
  27. Staley J. T. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95 19211942 [PubMed].
    [Google Scholar]
  28. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [View Article].
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46 502505 [View Article].
    [Google Scholar]
  31. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48 187194 [View Article] [PubMed].
    [Google Scholar]
  32. Yoon J.-H., Kang K. H., Park Y.-H. ( 2003;). Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53 449454 [View Article] [PubMed].
    [Google Scholar]
  33. Yoon J.-H., Kang S. J., Oh T. K. ( 2008;). Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 58 11981202 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000633
Loading
/content/journal/ijsem/10.1099/ijsem.0.000633
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed