1887

Abstract

Strain FOL01 was isolated from traditionally fermented Korean jogae jeotgal (fermented clams). Phylogenetic sequence analysis of the 16S rRNA gene from FOL01 revealed that it is closely related to FS61-1 and ATCC 33313 with 99.39 % and 98.50 % 16S rRNA gene sequence similarities, respectively. API and VITEK analyses showed that strain FOL01 could be separated from its nearest phylogenetic relatives with respect to carbohydrate fermentation and antibiotic resistance. Subsequent amplified rRNA gene restriction analysis of 16S rRNA genes and III-restriction enzyme profiling of genomic DNAs revealed different band patterns. In addition, DNA–DNA hybridization of genomic DNAs showed 63.9 % relatedness. Analysis of the composition of cellular fatty acids confirmed that strain FOL01 differs from its close relatives and supports the proposal to assign this organism to a novel species of the genus . Based on these results, strain FOL01 could be classified as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FOL01 ( = KCCM 43128 = JCM 30589).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000631
2015-12-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4674.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000631&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Björkroth K. J. , Schillinger U. , Geisen R. , Weiss N. , Hoste B. , Holzapfel W. H. , Korkeala H. J. , Vandamme P. . ( 2002;). Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples. Int J Syst Evol Microbiol 52: 141–148 [CrossRef] [PubMed].
    [Google Scholar]
  3. Choi H.-J. , Cheigh C.-I. , Kim S.-B. , Lee J.-C. , Lee D.-W. , Choi S.-W. , Park J.-M. , Pyun Y.-R. . ( 2002;). Weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. Int J Syst Evol Microbiol 52: 507–511 [CrossRef] [PubMed].
    [Google Scholar]
  4. Choi E. J. , Lee S. H. , Jung J. Y. , Jeon C. O. . ( 2013;). Brevibacterium jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 63: 3430–3436 [PubMed].[CrossRef]
    [Google Scholar]
  5. Collins M. D. , Samelis J. , Metaxopoulos J. , Wallbanks S. . ( 1993;). Taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J Appl Bacteriol 75: 595–603 [CrossRef] [PubMed].
    [Google Scholar]
  6. De Bruyne K. , Camu N. , Lefebvre K. , De Vuyst L. , Vandamme P. . ( 2008;). Weissella ghanensis sp. nov., isolated from a Ghanaian cocoa fermentation. Int J Syst Evol Microbiol 58: 2721–2725 [CrossRef] [PubMed].
    [Google Scholar]
  7. De Bruyne K. , Camu N. , De Vuyst L. , Vandamme P. . ( 2010;). Weissella fabaria sp. nov., from a Ghanaian cocoa fermentation. Int J Syst Evol Microbiol 60: 1999–2005 [CrossRef] [PubMed].
    [Google Scholar]
  8. Fusco V. , Quero G. M. , Cho G. S. , Kabisch J. , Meske D. , Neve H. , Bockelmann W. , Franz C. M. . ( 2015;). The genus Weissella: taxonomy, ecology and biotechnological potential. Front Microbiol 6: 155 [CrossRef] [PubMed].
    [Google Scholar]
  9. Gonzalez J. M. , Saiz-Jimenez C. . ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4: 770–773 [CrossRef] [PubMed].
    [Google Scholar]
  10. Guan L. , Cho K. H. , Lee J.-H. . ( 2011;). Analysis of the cultivable bacterial community in jeotgal, a Korean salted and fermented seafood, and identification of its dominant bacteria. Food Microbiol 28: 101–113 [CrossRef] [PubMed].
    [Google Scholar]
  11. Holzapfel W. H. , Gerber E. S. . ( 1983;). Lactobacillus divergens sp. nov., a new heterofermentative Lactobacillus species producing L(+)-lactate. Syst Appl Microbiol 4: 522–534 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kim H.-J. , Lee N. K. , Cho S. M. , Kim K. T. , Paik H. D. . ( 1999;). Inhibition of spoilage and pathogenic bacteria by lacticin NK24, a bacteriocin produced by Lactococcus lactis NK24 from fermented fish food. Korean J Food Sci Technol 31: 1035–1043.
    [Google Scholar]
  13. Kim O.-S. , Cho Y.-J. , Lee K. , Yoon S.-H. , Kim M. , Na H. , Park S.-C. , Jeon Y. S. , Lee J.-H. , other authors . ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kimura M. . ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120 [CrossRef] [PubMed].
    [Google Scholar]
  15. Lee J.-S. , Lee K. C. , Ahn J.-S. , Mheen T.-I. , Pyun Y.-R. , Park Y.-H. . ( 2002;). Weissella koreensis sp. nov., isolated from kimchi. Int J Syst Evol Microbiol 52: 1257–1261 [PubMed].
    [Google Scholar]
  16. Lee N. , Kim H. , Choi S. , Paik D. . ( 2003;). Some probiotic properties of some lactic acid bacteria and yeast isolated from jeot-gal. Kor J Microbiol Biotechnol 31: 297–300.
    [Google Scholar]
  17. Lee K. , Park J. , Chun J. , Han N. , Kim J. . ( 2010;). Importance of Weissella species during kimchi fermentation and future works. Kor J Microbiol Biotechnol 38: 341–348.
    [Google Scholar]
  18. Lee S. H. , Shim J. K. , Kim J. M. , Choi H.-K. , Jeon C. O. . et al., ( 2011;). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . Int J Syst Evol Microbiol 61: 722–727 [CrossRef] [PubMed].
    [Google Scholar]
  19. Lee S.-H. , Ahn M.-J. , Hong J.-S. , Lee J.-H. . ( 2015;). Diversity and community analysis of fermenting bacteria isolated from eight major Korean fermented foods using arbitrary-primed PCR and 16S rRNA gene sequencing. J Korean Soc Appl Biol Chem 58: 453–461 [CrossRef].
    [Google Scholar]
  20. Liu J. Y. , Li A. H. , Ji C. , Yang W. M. . ( 2009;). First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China. Vet Microbiol 136: 314–320 [CrossRef] [PubMed].
    [Google Scholar]
  21. Lu S. , Park M. , Ro H. S. , Lee D. S. , Park W. , Jeon C. O. . ( 2006;). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. J Microbiol 44: 155–161 [PubMed].
    [Google Scholar]
  22. Naser S. M. , Thompson F. L. , Hoste B. , Gevers D. , Dawyndt P. , Vancanneyt M. , Swings J. . ( 2005;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151: 2141–2150 [CrossRef] [PubMed].
    [Google Scholar]
  23. Nisiotou A. , Dourou D. , Filippousi M. E. , Banilas G. , Tassou C. . ( 2014;). Weissella uvarum sp. nov., isolated from wine grapes. Int J Syst Evol Microbiol 64: 3885–3890 [PubMed].[CrossRef]
    [Google Scholar]
  24. Padonou S. W. , Schillinger U. , Nielsen D. S. , Franz C. M. , Hansen M. , Hounhouigan J. D. , Nago M. C. , Jakobsen M. . ( 2010;). Weissella beninensis sp. nov., a motile lactic acid bacterium from submerged cassava fermentations, and emended description of the genus Weissella . Int J Syst Evol Microbiol 60: 2193–2198 [CrossRef] [PubMed].
    [Google Scholar]
  25. Parte A. C. . ( 2014;). LPSN-list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42: (D1), D613–D616.[CrossRef]
    [Google Scholar]
  26. Putnam L. R. , Howard W. J. , Pfaller M. A. , Koontz F. P. , Jones R. N. . ( 1997;). Accuracy of the Vitek system for antimicrobial susceptibility testing Enterobacteriaceae bloodstream infection isolates: use of direct inoculation from Bactec 9240 blood culture bottles. Diagn Microbiol Infect Dis 28: 101–104 [CrossRef] [PubMed].
    [Google Scholar]
  27. Sasser M. . ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  28. Snauwaert I. , Papalexandratou Z. , De Vuyst L. , Vandamme P. . ( 2013;). Characterization of strains of Weissella fabalis sp. nov. and Fructobacillus tropaeoli from spontaneous cocoa bean fermentations. . In Int J Syst Evol Microbiol 63: 1709–1716.[CrossRef]
    [Google Scholar]
  29. Tamura K. , Stecher G. , Peterson D. , Filipski A. , Kumar S. . ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 2725–2729 [CrossRef] [PubMed].
    [Google Scholar]
  30. Tanasupawat S. , Shida O. , Okada S. , Komagata K. . ( 2000;). Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50: 1479–1485 [CrossRef] [PubMed].
    [Google Scholar]
  31. Tohno M. , Kitahara M. , Inoue H. , Uegaki R. , Irisawa T. , Ohkuma M. , Tajima K. . ( 2013;). Weissella oryzae sp. nov., isolated from fermented rice grains. Int J Syst Evol Microbiol 63: 1417–1420 [CrossRef] [PubMed].
    [Google Scholar]
  32. Vela A. I. , Fernández A. , de Quirós Y. B. , Herráez P. , Domínguez L. , Fernández-Garayzábal J. F. . ( 2011;). Weissella ceti sp. nov., isolated from beaked whales (Mesoplodon bidens). Int J Syst Evol Microbiol 61: 2758–2762 [PubMed].[CrossRef]
    [Google Scholar]
  33. Walsh P. S. , Metzger D. A. , Higushi R. . ( 2013;). Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10(4): 506-13 (April 1991). Biotechniques 54: 134–139 [PubMed].
    [Google Scholar]
  34. Walter J. , Hertel C. , Tannock G. W. , Lis C. M. , Munro K. , Hammes W. P. . ( 2001;). Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67: 2578–2585 [CrossRef] [PubMed].
    [Google Scholar]
  35. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. , Moore E. , Murray R. , other authors . ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 37: 463–464.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000631
Loading
/content/journal/ijsem/10.1099/ijsem.0.000631
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error