A red-pigmented bacterium producing a metallic green sheen, designated strain JC333T, was isolated from a sand sample collected from Shivrajpur–Kachigad beach, Gujarat, India. Phylogenetic analyses based on the 16S rRNA gene sequence of strain JC333T showed highest sequence similarity to Zooshikella ganghwensis JC2044T (99.24 %) and less than 91.94 % similarity with other members of the class Gammaproteobacteria. DNA–DNA hybridizations between JC333T and Z. ganghwensis JC2044T showed low relatedness values of 19 ± 1.3 % (reciprocal 21 ± 2.2 %). The major respiratory quinone was ubiquinone-9 (Q9) and the polar lipid profile was composed of the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified lipid. The presence of C16 : 1ω7c/C16 : 1ω6c, C16 : 0, C18 : 1ω7c and C12 : 0 as major fatty acids supported the affiliation of strain JC333T to the genus Zooshikella. Prodigiosin, cycloprodigiosin and eight other prodigiosin analogues were the pigments of JC333T. Characterization based on 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, ubiquinone, and polar lipid and fatty acid compositions revealed that JC333T represents a novel species of the genus Zooshikella, for which the name Zooshikella marina sp. nov. is proposed. The type strain is JC333T ( = KCTC 42659T = LMG 28823T).
Published Online:
Funding
This study was supported by the:
Ministry of Earth Sciences
(Award MoES/16/06/2013)
KimuraM.
( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16111–120 [View Article][PubMed]
.
LeeJ. S.,
KimY. S.,
ParkS.,
KimJ.,
KangS. J.,
LeeM. H.,
RyuS.,
ChoiJ. M.,
OhT. K.,
YoonJ. H.
( 2011;). Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1. Appl Environ Microbiol774967–4973 [View Article][PubMed]
.
MesbahM.,
PremachandranU.,
WhitmanW. B.
( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol39159–167 [View Article].
RamaprasadE.V.V.,
SasikalaCh.,
RamanaCh.V.
( 2015b;). Flectobacillus rhizosphaerae sp. nov. isolated from the rhizosphere soil of Oryza sativa (L.) and emended description of the genus Flectobacillus
. Int J Syst Evol Microbiol [View Article].
StackebrandtE.,
GoebelB. M.
( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol44846–849. [CrossRef]
TusharL.,
SasikalaCh.,
RamanaCh.V.
( 2014;). Draft genome sequence of Rhodomicrobium udaipurense JA643T with special reference to hopanoid biosynthesis. DNA Res21639–647 [View Article][PubMed]
.
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP.A.D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW.E.C.,
MurrayR.G.E.,
other authors.
( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37463–464 [View Article].
YurkovV.,
StackebrandtE.,
HolmesA.,
FuerstJ. A.,
HugenholtzP.,
GoleckiJ.,
Gad'onN.,
GorlenkoV. M.,
KompantsevaE. I.,
DrewsG.
( 1994;). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol44427–434 [View Article][PubMed]
.