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Abstract

A red-pigmented bacterium producing a metallic green sheen, designated strain JC333, was isolated from a sand sample collected from Shivrajpur–Kachigad beach, Gujarat, India. Phylogenetic analyses based on the 16S rRNA gene sequence of strain JC333 showed highest sequence similarity to JC2044 (99.24 %) and less than 91.94 % similarity with other members of the class . DNA–DNA hybridizations between JC333 and JC2044 showed low relatedness values of 19 ± 1.3 % (reciprocal 21 ± 2.2 %). The major respiratory quinone was ubiquinone-9 (Q9) and the polar lipid profile was composed of the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified lipid. The presence of Cω7/Cω6, C, Cω7 and C as major fatty acids supported the affiliation of strain JC333 to the genus . Prodigiosin, cycloprodigiosin and eight other prodigiosin analogues were the pigments of JC333. Characterization based on 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, ubiquinone, and polar lipid and fatty acid compositions revealed that JC333 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC333 ( = KCTC 42659 = LMG 28823).

Funding
This study was supported by the:
  • Ministry of Earth Sciences (Award MoES/16/06/2013)
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/content/journal/ijsem/10.1099/ijsem.0.000630
2015-12-01
2024-10-13
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References

  1. Chakravarthy S. K., Ramaprasad E.V.V., Shobha E., Sasikala Ch., Ramana Ch.V. ( 2012;). Rhodoplanes piscinae sp. nov. isolated from pond water. Int J Syst Evol Microbiol 62 28282834 [View Article] [PubMed] .
    [Google Scholar]
  2. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  3. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed] .
    [Google Scholar]
  4. Lee J. S., Kim Y. S., Park S., Kim J., Kang S. J., Lee M. H., Ryu S., Choi J. M., Oh T. K., Yoon J. H. ( 2011;). Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1. Appl Environ Microbiol 77 49674973 [View Article] [PubMed] .
    [Google Scholar]
  5. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  6. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  7. Ramaprasad E.V.V., Sasikala Ch., Ramana Ch.V. ( 2015a;). Roseomonas oryzae sp. nov., isolated from paddy rhizosphere soil. Int J Syst Evol Microbiol [View Article].
    [Google Scholar]
  8. Ramaprasad E.V.V., Sasikala Ch., Ramana Ch.V. ( 2015b;). Flectobacillus rhizosphaerae sp. nov. isolated from the rhizosphere soil of Oryza sativa (L.) and emended description of the genus Flectobacillus . Int J Syst Evol Microbiol [View Article].
    [Google Scholar]
  9. Seldin L., Dubnau D. ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35 151154 [View Article].
    [Google Scholar]
  10. Stackebrandt E., Goebel B. M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44 846849. [CrossRef]
    [Google Scholar]
  11. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed] .
    [Google Scholar]
  12. Tushar L., Sasikala Ch., Ramana Ch.V. ( 2014;). Draft genome sequence of Rhodomicrobium udaipurense JA643T with special reference to hopanoid biosynthesis. DNA Res 21 639647 [View Article] [PubMed] .
    [Google Scholar]
  13. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  14. Yi H., Chang Y. H., Oh H. W., Bae K. S., Chun J. ( 2003;). Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. Int J Syst Evol Microbiol 53 10131018 [View Article] [PubMed] .
    [Google Scholar]
  15. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad'on N., Gorlenko V. M., Kompantseva E. I., Drews G. ( 1994;). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44 427434 [View Article] [PubMed] .
    [Google Scholar]
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