1887

Abstract

Two Gram-stain negative, coccoid to oval-shaped, non-spore-forming bacteria (LR4 and LR4-1), isolated from the soil of a pesticide factory in Nanjing, China, were investigated for their taxonomic allocation by using a polyphasic approach. Both strains grew optimally at pH 7.0, 30 °C and in the absence of NaCl. Both strains were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and two unknown aminolipids. The major fatty acids (>10 % of the total fatty acids) were Cω7/Cω6 (summed feature 8) and C iso I/C anteiso B (summed feature 4). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the two isolates formed a distinct line within a clade containing the genera , , , , , , , , and in the order , with the highest 16S rRNA gene sequence similarity to TE2 (94.12 %), followed by DSM 9653 (93.25 %). Strains LR4 and LR4-1 were closely related on the basis of DNA–DNA reassociation and therefore represent a single novel species. Based on phenotypic, chemotaxonomic and phylogenetic data, strains LR4 and LR4-1 represent a novel species of a new genus in the order , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is LR4 ( = CCTCC AB 2015036 = KCTC 42463).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000620
2015-12-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4608.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000620&mimeType=html&fmt=ahah

References

  1. Auling G., Busse H.-J., Egli T., El-Banna T., Stackebrandt E.. ( 1993;). Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov. Syst Appl Microbiol 16: 104–112 [CrossRef].
    [Google Scholar]
  2. Bertani G.. ( 1951;). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol 62: 293–300 [PubMed].
    [Google Scholar]
  3. Beveridge T. J., Lawrence J. R., Murray R. G. E.. ( 2007;). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, pp. 19–33. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R.., 3rd edn. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  4. Das S. K., Mishra A. K., Tindall B. J., Rainey F. A., Stackebrandt E.. ( 1996;). Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46: 981–987 [CrossRef] [PubMed].
    [Google Scholar]
  5. De Meyer S. E., Willems A.. ( 2012;). Multilocus sequence analysis of Bosea species and description of Bosea lupini sp. nov.,Bosea lathyri sp. nov. and Bosea robiniae sp. nov., isolated from legumes. Int J Syst Evol Microbiol 62: 2505–2510 [CrossRef] [PubMed].
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224–229 [CrossRef].
    [Google Scholar]
  7. He L., Li W., Huang Y., Wang L., Liu Z., Lanoot B., Vancanneyt M., Swings J.. ( 2005;). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55: 1939–1944 [CrossRef] [PubMed].
    [Google Scholar]
  8. Kämpfer P., Scholz H. C., Langer S., Wernery U., Wernery R., Johnson B., Joseph M., Lodders N., Busse H.-J.. ( 2010;). Camelimonas lactis gen. nov., sp. nov., isolated from the milk of camels. Int J Syst Evol Microbiol 60: 2382–2386 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kämpfer P., Scholz H. C., Lodders N., Loncaric I., Whatmore A. M., Busse H.-J.. ( 2012;). Camelimonas abortus sp. nov., isolated from placental tissue of cattle. Int J Syst Evol Microbiol 62: 1117–1120 [CrossRef] [PubMed].
    [Google Scholar]
  10. Kämpfer P., Jerzak L., Wilharm G., Golke J., Busse H. J., Glaeser S. P.. ( 2015;). Gemmobacter intermedius sp. nov., isolated from a white stork (Ciconia ciconia). Int J Syst Evol Microbiol 65: 778–783 [CrossRef] [PubMed].
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  12. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38: 358–361 [CrossRef].
    [Google Scholar]
  13. La Scola B., Mallet M. N., Grimont P. A. D., Raoult D.. ( 2003;). Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das .1996). Int J Syst Evol Microbiol 53: 15–20 [CrossRef] [PubMed].
    [Google Scholar]
  14. Lane D. L.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. R., Goodfellow M.. Chichester: Wiley;.
    [Google Scholar]
  15. Lee K. B., Liu C. T., Anzai Y., Kim H., Aono T., Oyaizu H.. ( 2005;). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov. Int J Syst Evol Microbiol 55: 1907–1919 [CrossRef] [PubMed].
    [Google Scholar]
  16. Liu J. H., Wang Y. X., Zhang X. X., Wang Z. G., Chen Y. G., Wen M. L., Xu L. H., Peng Q., Cui X. L.. ( 2010;). Salinarimonas rosea gen. nov., sp. nov., a new member of the α-2 subgroup of the Proteobacteria. Int J Syst Evol Microbiol 60: 55–60 [CrossRef] [PubMed].
    [Google Scholar]
  17. Liu X. M., Chen K., Meng C., Zhang L., Zhu J. C., Huang X., Li S. P., Jiang J. D.. ( 2014;). Pseudoxanthobacter liyangensis sp. nov., isolated from dichlorodiphenyltrichloroethane-contaminated soil. Int J Syst Evol Microbiol 64: 3390–3394 [CrossRef] [PubMed].
    [Google Scholar]
  18. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner,A., Lai T., Steppi S., other authors. ( 2004;). arb: a software environment for sequence data. Nucleic Acids Res 32: 1363–1371 [CrossRef] [PubMed].
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39: 159–167 [CrossRef].
    [Google Scholar]
  20. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16: 584–586 [PubMed].
    [Google Scholar]
  21. Panday D., Das S. K.. ( 2010;). Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment. Int J Syst Evol Microbiol 60: 861–865 [CrossRef] [PubMed].
    [Google Scholar]
  22. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28: 1823–1829 [CrossRef] [PubMed].
    [Google Scholar]
  23. Stamatakis A.. ( 2006;). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690 [CrossRef] [PubMed].
    [Google Scholar]
  24. Tel-Zur N., Abbo S., Myslabodski D., Mizrahi Y., Modified C.T.A.B.. ( 1999;). procedure for DNA isolation from epiphytic cacti of the genera Hylocereus and Selenicereus (Cactaceae). Plant Mol Biol Rep 17: 249–254 [CrossRef].
    [Google Scholar]
  25. Tindall B. J.. ( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13: 128–130 [CrossRef].
    [Google Scholar]
  26. Tindall B. J.. ( 1990b;). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66: 199–202 [CrossRef].
    [Google Scholar]
  27. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J., Marzluf G. A., Schmidt T. M., Snyder L. R.., 3rd edn.., Washington, DC: American Society for Microbiology; [CrossRef].
    [Google Scholar]
  28. Verma M., Kumar M., Dadhwal M., Kaur J., Lal R.. ( 2009;). Devosia albogilva sp. nov. and Devosia crocina sp. nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 59: 795–799 [CrossRef] [PubMed].
    [Google Scholar]
  29. Woese C. R., Stackebrandt E., Weisburg W. G., Paster B. J., Madigan M. T., Fowler V. J., Hahn C. M., Blanz P., Gupta R., other authors. ( 1984;). The phylogeny of purple bacteria: the alpha subdivision. Syst Appl Microbiol 5: 315–326 [CrossRef] [PubMed].
    [Google Scholar]
  30. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R.. ( 2008;). The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31: 241–250 [CrossRef] [PubMed].
    [Google Scholar]
  31. Yoon J.-H., Kang S.-J., Im W.-T., Lee S.-T., Oh T.-K.. ( 2008;). Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus. Int J Syst Evol Microbiol 58: 2224–2228 [CrossRef] [PubMed].
    [Google Scholar]
  32. Zhang J., Gu T., Zhou Y., He J., Zheng L. Q., Li W. J., Huang X., Li S. P.. ( 2012;). Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas. Int J Syst Evol Microbiol 62: 2593–2597 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000620
Loading
/content/journal/ijsem/10.1099/ijsem.0.000620
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error