1887

Abstract

Two Gram-stain negative, coccoid to oval-shaped, non-spore-forming bacteria (LR4 and LR4-1), isolated from the soil of a pesticide factory in Nanjing, China, were investigated for their taxonomic allocation by using a polyphasic approach. Both strains grew optimally at pH 7.0, 30 °C and in the absence of NaCl. Both strains were positive for catalase and oxidase activities. Q-10 was the predominant respiratory ubiquinone. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and two unknown aminolipids. The major fatty acids (>10 % of the total fatty acids) were Cω7/Cω6 (summed feature 8) and C iso I/C anteiso B (summed feature 4). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the two isolates formed a distinct line within a clade containing the genera , , , , , , , , and in the order , with the highest 16S rRNA gene sequence similarity to TE2 (94.12 %), followed by DSM 9653 (93.25 %). Strains LR4 and LR4-1 were closely related on the basis of DNA–DNA reassociation and therefore represent a single novel species. Based on phenotypic, chemotaxonomic and phylogenetic data, strains LR4 and LR4-1 represent a novel species of a new genus in the order , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is LR4 ( = CCTCC AB 2015036 = KCTC 42463).

Funding
This study was supported by the:
  • , Outstanding Youth Foundation of Jiangsu Province , (Award BK20130029)
  • , the Chinese National Science Foundation for Excellent Young Scholars (31222003)
  • , the Program for New Century Excellent Talents in University (NCET-12-0892)
  • , the Fundamental Research Funds for the Central Universities (KYZ201422; KJQN201529)
  • , the National Natural Science Foundation of China (31400105)
  • , the China Postdoctoral Science Foundation (2014M561666)
  • , the International Science & Technology Cooperation Program of China (2013DFR30920)
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2015-12-01
2020-09-28
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