1887

Abstract

A Gram-stain-negative spirochaete (strain JC202) was isolated from the gut of the termite (Rambur) from Rann of Kutch, Gujarat, India. This strain was obligately anaerobic, mesophilic, halotolerant and required alkaline conditions for growth. Strain JC202 was resistant to rifampicin and kanamycin, but sensitive to gentamicin, tetracycline, ampicillin and chloramphenicol. Strain JC202 possessed phosphatidylglycerol, diphosphatidylglycerol, glycolipid and six unidentified lipids. Cω7 was the predominant cellular fatty acid with significant proportions of C, Cω9, C, C, Cω5, Cω5 and Cω9. The DNA G+C content of strain JC202 was 59 mol%. Based on 16S rRNA gene sequence analysis, strain JC202 is considered to belong to the genus with JC133 (100 % similarity), Z-7491 (99.92 %), ATCC BAA-392 (99.47 %) and other members of the genus ( < 93.83 %) as the closest phylogenetic neighbours. However, mean DNA–DNA hydridization values between strain JC202 and JC133, DSM 8900 ( = Z-7491) and DSM 14872 ( = ASpG1) were 55 ± 2, 22 ± 3 and 32 ± 1 %, respectively. On the basis of physiological, biochemical, chemotaxonomic (including metabolome) and genomic differences from the previously described taxa, strain JC202 is differentiated from other members of the genus and is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is JC202 ( = KCTC 15324 = NBRC 110104).

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2015-12-01
2021-03-01
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