1887

Abstract

A novel strain (designated sjH1), characterized as aerobic, Gram-stain-negative, oxidase-positive, catalase-negative, motile and rod-shaped, was isolated from mine wastewater. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sjH1 belonged to the genus . Strain sjH1 was closely related to LCS2 (98.0% 16S rRNA gene sequence similarity), 2APBS1 (97.7%), DCY45 (97.2%) and MJ01 (97.0%). The DNA G+C content of strain sjH1 was 69.2 mol%. DNA–DNA relatedness ( < 60%) indicated that strain sjH1 represents a distinct species that is separate from , , and . The major ubiquinone was Q-8, and major fatty acids were summed feature 9 (iso–Cω9 and/or C 10-methyl), iso-C, iso-C, iso-C and anteiso-C. Based on data from this polyphasic study, it is proposed that sjH1 ( = KCTC 42660 = JCM 30774) is the type strain of a novel species, sp. nov.

Funding
This study was supported by the:
  • the survey of indigenous species of Korea of the National Institute of Biological Resources under the Ministry of Environment (Korea) (Award 20132020000170)
  • a research grant from Chungbuk National University (2014)
  • the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) granted financial resources by the Ministry of Trade, Industry & Energy, Republic of Korea
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2015-12-01
2021-04-11
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