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Abstract

Strain CBA3205 is a Gram-stain-negative, non-motile and rod-shaped bacterium that was isolated from the seashore sand of Jeju Island in South Korea. Based on the phylogenetic analysis, the most closed related species was DOKDO 025, with 94.8 % sequence similarity for the 16S rRNA gene. Strain CBA3205 was observed to grow optimally at 25–30 °C and at pH 8.5 in the presence of 2–3 % (w/v) NaCl. The major fatty acids of strain CBA3205 were iso-C, iso-C G, and iso-C 3-OH. The major respiratory quinone was MK-6 and the major polar lipids were two unidentified amino-group-containing phospholipids and an unidentified polar lipid. The G+C content of the genomic DNA of strain CBA3205 was 62.5 mol%. Based on the phenotypic, genotypic and phylogenetic analyses, strain CBA3205 was considered to be a novel species belonging to the genus within the family , for which the name sp. nov. is proposed. The type strain is CBA3205 ( = KACC 17669 = JCM 19531).

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2015-12-01
2019-12-07
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References

  1. Agarwal S., Hunnicutt D. W., McBride M. J.. ( 1997;). Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA. Proc Natl Acad Sci U S A 94: 12139–12144 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y.. ( 2006;). An introduction to the family Flavobacteriaceae. . In The Prokaryotes, a Handbook on the Biology of Bacteria, 3rd edn., vol. 7, pp. 455–480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E.. New York: Springer;.
    [Google Scholar]
  3. Bernardet J. F., Nakagawa Y., Holmes B., Subcommittee on the Taxonomy of Flavobacterium and Cytophaga-like Bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Subcommittee on the Taxonomy of Flavobacterium and Cytophaga-like Bacteria of the International Committee on Systematics of ProkaryotesProposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  4. Collins M. D., Jones D.. ( 1981a;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45: 316–354 [PubMed].
    [Google Scholar]
  5. Collins M. D., Jones D.. ( 1981b;). A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51: 129–134 [CrossRef] [PubMed].
    [Google Scholar]
  6. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17: 368–376 [CrossRef] [PubMed].
    [Google Scholar]
  7. Garrity G. M., Holt J. G.. ( 2001;). The road map to the Manual. . In Bergey's Manual of Systematic Bacteriology, 2nd edn., vol. 1, pp. 119–166. Edited by Boone D. R., Castenholz R. W., Garrity G. M.. New York: Springer; [CrossRef].
    [Google Scholar]
  8. González C., Gutierrez C., Ramirez C.. ( 1978;). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24: 710–715 [CrossRef] [PubMed].
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  10. Kluge A. G., Farris F. S.. ( 1969;). Quantitative phyletics and the evolution of anurans. Syst Biol 18: 1–32 [CrossRef].
    [Google Scholar]
  11. Lee H. S., Kwon K. K., Yang S. H., Bae S. S., Park C. H., Kim S. J., Lee J. H.. ( 2008;). Description of Croceitalea gen. nov. in the family Flavobacteriaceae with two species, Croceitalea eckloniae sp. nov. and Croceitalea dokdonensis sp. nov., isolated from the rhizosphere of the marine alga Ecklonia kurome. Int J Syst Evol Microbiol 58: 2505–2510 [CrossRef] [PubMed].
    [Google Scholar]
  12. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479: 297–306 [CrossRef] [PubMed].
    [Google Scholar]
  13. MIDI ( 1999;). Sherlock Microbial Identification System Operating Manual, version 3.0 Newark, DE: MIDI, Inc;.
    [Google Scholar]
  14. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  15. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28: 1823–1829 [CrossRef] [PubMed]
    [Google Scholar]
  16. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  17. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, p. 607. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  19. Tittsler R. P., Sandholzer L. A.. ( 1936;). The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31: 575–580 [PubMed].
    [Google Scholar]
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