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Abstract

A Gram-stain-negative, non-spore-forming, short rod-shaped, non-motile, light-pink bacterial strain, MIMtkLc17, was isolated from biological soil crusts collected in Liangcheng, Inner Mongolia. Growth of strain MIMtkLc17 was observed at 2–35 °C and in the presence of 1% (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences showed that sequence similarities between strain MIMtkLc17 and the type strains of species of the genus ranged from 89.93% to 96.49%. Strain MIMtkLc17 can secrete mass polysaccharide. The major fatty acids of strain MIMtkLc17 were iso-C, summed feature 3 (Cω7/Cω6), Cω5 and summed feature 4 (iso-C I/anteiso-C B). The sole respiratory quinone was menaquinone MK-7. The G+C content of the chromosomal DNA was 57.8 mol%. The results of phylogenetic, chemotaxonomic and phenotypic characterization indicated that strain MIMtkLc17 can be distinguished from all known species of the genus and represents a novel species of this genus, for which the name sp. nov. is proposed. The type strain is MIMtkLc17 ( = MCCC 1K00507 = KCTC 42636).

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2015-12-01
2019-12-12
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bauer A. W., Kirby W. M., Sherris J. C., Turck M.. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496 [PubMed].
    [Google Scholar]
  3. Buck J. D.. ( 1982;). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44: 992–993 [PubMed].
    [Google Scholar]
  4. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57: 2259–2261 [CrossRef] [PubMed].
    [Google Scholar]
  5. Concostrina-Zubiri L., Huber-Sannwald E., Martínez I., Flores Flores J. L. F., Escudero A.. ( 2013;). Biological soil crusts greatly contribute to small-scale soil heterogeneity along a grazing gradient. Soil Biol Biochem 64: 28–36 [CrossRef].
    [Google Scholar]
  6. Dong X., Cai M.. ( 2001;). Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);.
    [Google Scholar]
  7. Du H., Jiao N., Hu Y., Zeng Y.. ( 2006;). Diversity and distribution of pigmented heterotrophic bacteria in marine environments. FEMS Microbiol Ecol 57: 92–105 [CrossRef] [PubMed].
    [Google Scholar]
  8. Du Y., Yuan B., Zeng Y., Meng J., Li H., Wang R., Li G., Feng F.. ( 2015;). Draft genome sequence of the cellulolytic bacterium Clavibacter sp., CF11, a strain producing cold-active cellulase. Genome Announc 3: e01304–e01314 [CrossRef] [PubMed].
    [Google Scholar]
  9. Edgar R. C.. ( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797 [CrossRef] [PubMed].
    [Google Scholar]
  10. Han L., Wu S.-J., Qin C.-Y., Zhu Y.-H., Lu Z.-Q., Xie B., Lv J.. ( 2014;). Hymenobacter qilianensis sp. nov., isolated from a subsurface sandstone sediment in the permafrost region of Qilian Mountains, China and emended description of the genus Hymenobacter. Antonie van Leeuwenhoek 105: 971–978 [CrossRef] [PubMed].
    [Google Scholar]
  11. Hirsch P., Ludwig W., Hethke C., Sittig M., Hoffmann B., Gallikowski C. A.. ( 1998;). Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctic soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. Syst Appl Microbiol 21: 374–383 [CrossRef] [PubMed].
    [Google Scholar]
  12. Hoang V.-A., Kim Y.-J., Nguyen N. L., Yang D.-C.. ( 2013;). Hymenobacter ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 63: 661–666 [CrossRef] [PubMed].
    [Google Scholar]
  13. Jin L., Lee H. G., Kim S. G., Lee K. C., Ahn C. Y., Oh H. M.. ( 2014;). Hymenobacter ruber sp. nov., isolated from grass soil. Int J Syst Evol Microbiol 64: 979–983 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kim K. H., Im W. T., Lee S. T.. ( 2008;). Hymenobacter soli sp. nov., isolated from grass soil. Int J Syst Evol Microbiol 58: 941–945 [CrossRef] [PubMed].
    [Google Scholar]
  15. Kimura M., Takahata N.. ( 1983;). Selective constraint in protein polymorphism: study of the effectively neutral mutation model by using an improved pseudosampling method. Proc Natl Acad Sci U S A 80: 1048–1052 [CrossRef] [PubMed].
    [Google Scholar]
  16. Klassen J. L., Foght J. M.. ( 2011;). Characterization of Hymenobacter isolates from Victoria Upper Glacier, Antarctica reveals five new species and substantial non-vertical evolution within this genus. Extremophiles 15: 45–57 [CrossRef] [PubMed].
    [Google Scholar]
  17. Lee J. J., Srinivasan S., Lim S., Joe M., Lee S. H., Kwon S. A., Kwon Y. J., Lee J., Choi J. J., other authors. ( 2014;). Hymenobacter swuensis sp. nov., a gamma-radiation-resistant bacteria isolated from mountain soil. Curr Microbiol 68: 305–310 [CrossRef] [PubMed].
    [Google Scholar]
  18. Liu L., Zhou E.-M., Jiao J.-Y., Manikprabhu D., Ming H., Liu W.-H., Hozzein W. N., Shu W.-S., Li W.-J.. ( 2015;). Hymenobacter latericoloratus sp. nov. and Hymenobacter luteus sp. nov., isolated from freshwater sediment. Antonie van Leeuwenhoek 107: 165–172 [CrossRef] [PubMed].
    [Google Scholar]
  19. Reddy G. S., Garcia-Pichel F.. ( 2013;). Description of Hymenobacter arizonensis sp. nov. from the southwestern arid lands of the United States of America. Antonie van Leeuwenhoek 103: 321–330 [CrossRef] [PubMed].
    [Google Scholar]
  20. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  21. Skerman V.. ( 1967;). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  22. Srinivasan S., Joo E. S., Lee J.-J., Kim M. K.. ( 2015a;). Hymenobacter humi sp. nov., a bacterium isolated from soil. Antonie van Leeuwenhoek 107: 1411–1419 [CrossRef] [PubMed].
    [Google Scholar]
  23. Srinivasan S., Lee J. J., Park K. R., Park S. H., Jung H. Y., Kim M. K.. ( 2015b;). Hymenobacter terrae sp. nov., a bacterium isolated from soil. Curr Microbiol 70: 643–650 [CrossRef] [PubMed].
    [Google Scholar]
  24. Su S., Chen M., Teng C., Jiang S., Zhang C., Lin M., Zhang W.. ( 2014;). Hymenobacter kanuolensis sp. nov., a novel radiation-resistant bacterium. Int J Syst Evol Microbiol 64: 2108–2112 [CrossRef] [PubMed].
    [Google Scholar]
  25. Subhash Y., Sasikala Ch., Ramana ChV. ( 2014;). Hymenobacter roseus sp. nov., isolated from sand. Int J Syst Evol Microbiol 64: 4129–4133 [CrossRef] [PubMed].
    [Google Scholar]
  26. Swindell S. R., Plasterer T. N.. ( 1997;). seqman. . In Sequence Data Analysis Guidebook (Methods in Molecular Medicine, vol. 70), pp. 75–89. Edited by Swindell S. R.. New York: Springer; [CrossRef].
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  28. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. [CrossRef] In Methods for General and Molecular Microbiology, 3rd edn, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  29. Xu J. L., Liu Q. M., Yu H. S., Jin F. X., Lee S. T., Im W. T.. ( 2009;). Hymenobacter daecheongensis sp. nov., isolated from stream sediment. Int J Syst Evol Microbiol 59: 1183–1187 [CrossRef] [PubMed].
    [Google Scholar]
  30. Zhang Q., Liu C., Tang Y., Zhou G., Shen P., Fang C., Yokota A.. ( 2007;). Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 57: 1752–1756 [CrossRef] [PubMed].
    [Google Scholar]
  31. Zhang L., Dai J., Tang Y., Luo X., Wang Y., An H., Fang C., Zhang C.. ( 2009;). Hymenobacter deserti sp. nov., isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 59: 77–82 [CrossRef] [PubMed].
    [Google Scholar]
  32. Zhang D.-C., Busse H.-J., Liu H.-C., Zhou Y.-G., Schinner F., Margesin R.. ( 2011;). Hymenobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from soil. Int J Syst Evol Microbiol 61: 859–863 [CrossRef] [PubMed].
    [Google Scholar]
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