RT Journal Article SR Electronic(1) A1 Sammra, Osama A1 Balbutskaya, Anna A1 Ülbegi-Mohyla, Hivda A1 Nagib, Samy A1 Lämmler, Christoph A1 Kämpfer, Peter A1 Glaeser, Stefanie P. A1 Golke, Jan A1 Busse, Hans-Jürgen A1 Prenger-Berninghoff, Ellen A1 Siebert, Ursula A1 Abdulmawjood, Amir A1 Klein, GünterYR 2015 T1 Arcanobacterium pinnipediorum sp. nov., isolated from a harbour seal JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_12 SP 4539 OP 4543 DO https://doi.org/10.1099/ijsem.0.000609 PB Microbiology Society, SN 1466-5034, AB A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like, Gram-stain-positive bacterium, strain 2710T, isolated from a harbour seal. Comparative 16S rRNA gene sequence analysis showed that this bacterial strain belonged to the genus Arcanobacterium and was related most closely to the type strains of Arcanobacterium phocae (98.4 % similarity) and Arcanobacterium phocisimile (97.5 %). 16S rRNA gene sequence similarities to the type strains of other Arcanobacterium species were between 95.3 and 96.9 %. DNA–DNA hybridization values between strain 2710T and A. phocae DSM 10002T and A. phocisimile LMG 27073T were 4.7 % (reciprocal 56 %) and 23 % (reciprocal 7.7 %), respectively. The presence of the major menaquinone MK-9(H4) and a polar lipid profile with the major compounds diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside supported the affiliation of strain 2710T to the genus Arcanobacterium. The major fatty acids were C16:0, C18:1ω9c, C18:0 and C18:2ω6,9c/anteiso-C18:0. The peptidoglycan structure was of cross-linkage type A5α (l-Lys–l-Lys–d-Glu). Physiological and biochemical tests clearly distinguished the isolate from other members of the genus Arcanobacterium. Based on these tests, it is proposed that this unknown bacterium should be classified as a novel species of the genus Arcanobacterium, with the name Arcanobacterium pinnipediorum sp. nov. The type strain is 2710T ( = DSM 28752T = LMG 28298T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000609