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Abstract

The bacterial strain, IR-2, was isolated from a microbial mat sampled near a hydrothermal vent in the Greenland Sea. Phylogenetic analysis, based on the 16S rRNA gene, showed that the closest relatives of IR-2 were , and (91 % 16S rRNA gene sequence similarity). The cells of the novel strain were Gram-stain-negative and pleomorphic; changing from long motile rods to non-motile ring structures during the growth cycle. Growth occurred at 20–55 °C (optimally at 48 °C), with 1–6 % (w/v) NaCl (optimally with 2 %), and at pH 5.3–8.0 (optimally at pH 6.0–8.0). The strain had obligate fermentative growth on various sugars and yeast extract. The DNA G+C content of strain IR-2 was 25.7 mol%. The cell sugars comprised mainly ribose, mannose and glucose, while the main polar lipids were glycolipids, phospholipids, phosphatidylglycerol and diphosphatidylglycerol. The fatty acid content of strain IR-2 was dominated by saturated and unsaturated iso-branched or anteiso-branched forms. Strain IR-2 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is IR-2 ( = DSM 100055 = JCM 30901).

Funding
This study was supported by the:
  • , the Norwegian Research Council , (Award 208491)
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2015-12-01
2020-09-29
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