1887

Abstract

A Gram-stain-negative, non-spore-forming, rod-shaped, motile, facultatively anaerobic bacterium, designated CAU 1059, was isolated from a seawater sample from Jeju Island, Republic of Korea. The bacterium grew optimally at 37 °C, at pH 7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1059 belonged to the genus . It exhibited only 91.5–96.9 % sequence similarity to the type strains of recognized species. Similar to other species of the genus , strain CAU 1059 had ubiquinone-10 (Q-10) as the predominant ubiquinone and C and summed feature 8 (Cω7c/ω6c) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; three unidentified phospholipids, two aminolipids, an aminophospholipid and nine other lipids were also found. The G+C content of the genomic DNA was 61.9 mol%. On the basis of the data provided, strain CAU 1059 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 1059 ( = KCTC 32014 = CCUG 64792).

Funding
This study was supported by the:
  • Korea of the National Institute of Biological Resources (NIBR)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000604
2015-12-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4514.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000604&mimeType=html&fmt=ahah

References

  1. Biebl H., Allgaier M., Lünsdorf H., Pukall R., Tindall B. J., Wagner-Döbler I. ( 2005;). Roseovarius mucosus sp. nov., a member of the Roseobacter clade with trace amounts of bacteriochlorophyll a. Int J Syst Evol Microbiol 55 23772383 [View Article] [PubMed].
    [Google Scholar]
  2. Boettcher K. J., Geaghan K. K., Maloy A. P., Barber B. J. ( 2005;). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55 15311537 [View Article] [PubMed].
    [Google Scholar]
  3. Bowman J. P. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50 18611868 [View Article] [PubMed].
    [Google Scholar]
  4. Cappuccino J. G., Sherman N. ( 2002). Microbiology: a Laboratory Manual , 6th edn. Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  5. Choi E. J., Lee H. J., Kim J. M., Jeon C. O. ( 2013;). Roseovarius lutimaris sp. nov., isolated from a marine tidal flat. Int J Syst Evol Microbiol 63 38353840 [View Article] [PubMed].
    [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  8. Felsenstein J. ( 1989;). PHYLIP - phylogeny inference package (version3.2). Cladistics 5 164166.
    [Google Scholar]
  9. Fitch W. M., Margoliash E. ( 1967;). Construction of phylogenetic trees. Science 155 279284 [View Article] [PubMed].
    [Google Scholar]
  10. González J. M., Covert J. S., Whitman W. B., Henriksen J. R., Mayer F., Scharf B., Schmitt R., Buchan A., Fuhrman J. A., other authors. ( 2003;). Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments. Int J Syst Evol Microbiol 53 12611269 [View Article] [PubMed].
    [Google Scholar]
  11. Gordon R. E., Mihm J. M. ( 1962;). Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98 628636 [View Article].
    [Google Scholar]
  12. Goris J., Suzuki K. I., De Vos P., Nakase T., Kersters K. ( 1998;). Evaluation of a microplate DNA:DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44 11481153 [View Article].
    [Google Scholar]
  13. Jukes T. H., Cantor C. R. ( 1969;). Evolution of protein molecules. [View Article] In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. H. New York: Academic Press;.
    [Google Scholar]
  14. Jung Y.-T., Lee J. S., Oh K.-H., Oh T.-K., Yoon J.-H. ( 2011;). Roseovarius marinus sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61 427432 [View Article] [PubMed].
    [Google Scholar]
  15. Jung Y.-T., Park S., Yoon J.-H. ( 2012;). Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula. Antonie van Leeuwenhoek 102 141148 [View Article] [PubMed].
    [Google Scholar]
  16. Kim Y. O., Kong H. J., Park S., Kang S. J., Kim W. J., Kim K. K., Oh T. K., Yoon J. H. ( 2012;). Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi . Int J Syst Evol Microbiol 62 931936 [View Article] [PubMed].
    [Google Scholar]
  17. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161208 [View Article].
    [Google Scholar]
  18. Labrenz M., Collins M. D., Lawson P. A., Tindall B. J., Schumann P., Hirsch P. ( 1999;). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49 137147 [View Article] [PubMed].
    [Google Scholar]
  19. Lai Q., Zhong H., Wang J., Yuan J., Sun F., Wang L., Zheng T., Shao Z. ( 2011;). Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean. Int J Syst Evol Microbiol 61 20402044 [View Article] [PubMed].
    [Google Scholar]
  20. Lane D. J. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. London: Wiley;.
    [Google Scholar]
  21. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. ( 2007;). clustal w clustal_x version 2.0. Bioinformatics 23 29472948 [View Article] [PubMed].
    [Google Scholar]
  22. Li Z., Zhao R., Ji S., Shi X., Zhang X. H. ( 2013;). Roseovarius marisflavi sp. nov., isolated from an amphioxus breeding zone in the coastal region of the Yellow Sea, China. Antonie van Leeuwenhoek 104 413421 [View Article] [PubMed].
    [Google Scholar]
  23. Lucena T., Ruvira M. A., Macián M. C., Pujalte M. J., Arahal D. R. ( 2014;). Roseovarius albus sp. nov., a new Alphaproteobacterium isolated from the Mediterranean Sea. Antonie van Leeuwenhoek 105 671678 [View Article] [PubMed].
    [Google Scholar]
  24. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208218 [View Article].
    [Google Scholar]
  25. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188 221233 [View Article].
    [Google Scholar]
  26. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  27. Nam S. W., Kim W., Chun J., Goodfellow M. ( 2004;). Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 54 12091212 [View Article] [PubMed].
    [Google Scholar]
  28. Nicholson W. L., Setlow P. ( 1990;). Sporulation, germination and outgrowth. . In Molecular Biological Methods for Bacillus, pp. 391450. Edited by Harwood C. R., Cutting S. M. Chichester: Wiley;.
    [Google Scholar]
  29. Oh Y.-S., Lim H.-J., Cha I.-T., Im W.-T., Yoo J.-S., Kang U. G., Rhee S.-K., Roh D.-H. ( 2009;). Roseovarius halotolerans sp. nov., isolated from deep seawater. Int J Syst Evol Microbiol 59 27182723 [View Article] [PubMed].
    [Google Scholar]
  30. Oren A. ( 2010;). Industrial and environmental applications of halophilic microorganisms. Environ Technol 31 825834 [View Article] [PubMed].
    [Google Scholar]
  31. Park S., Yoon J.-H. ( 2013;). Roseovarius sediminilitoris sp. nov., isolated from seashore sediment. Int J Syst Evol Microbiol 63 17411745 [View Article] [PubMed].
    [Google Scholar]
  32. Park S., Park J.-M., Kang C.-H., Yoon J.-H. ( 2014;). Roseovarius gaetbuli sp. nov., a novel alphaproteobacterium isolated from a tidal flat sediment. Antonie van Leeuwenhoek 105 723730 [View Article] [PubMed].
    [Google Scholar]
  33. Parte A. C. ( 2014;). LPSN—list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42 (D1), D613D616 [View Article] [PubMed].
    [Google Scholar]
  34. Rajasabapathy R., Mohandass C., Dastager S. G., Liu Q., Khieu T.-N., Son C. K., Li W.-J., Colaco A. ( 2014;). Roseovarius azorensis sp. nov., isolated from seawater at Espalamaca, Azores. Antonie van Leeuwenhoek 105 571578 [View Article] [PubMed].
    [Google Scholar]
  35. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  36. Shivanand P., Mugeraya G. ( 2011;). Halophilic bacteria and their compatible solutes - osmoregulation and potential applications. Curr Sci 100 15161521.
    [Google Scholar]
  37. Smibert R. M., Krieg N. R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  38. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [View Article].
    [Google Scholar]
  39. Wang B., Tan T., Shao Z. ( 2009;). Roseovarius pacificus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 59 11161121 [View Article] [PubMed].
    [Google Scholar]
  40. Wang B., Sun F., Lai Q., Du Y., Liu X., Li G., Luo J., Shao Z. ( 2010;). Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment. Int J Syst Evol Microbiol 60 12891295 [View Article] [PubMed].
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [View Article].
    [Google Scholar]
  42. Yoon J.-H., Kang S. J., Oh T. K. ( 2008;). Roseovarius aestuarii sp. nov. isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 58 11981202 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000604
Loading
/content/journal/ijsem/10.1099/ijsem.0.000604
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error