1887

Abstract

A novel, Gram-negative marine bacterium, S2753, was isolated from a mussel of the Solomon Sea, Solomon Islands. Analysis of the 16S rRNA gene sequence and whole genome sequence data placed strain S2753 in the genus with the closest relative being DSM 18316 (97.7 % 16S rRNA gene similarity). Strain S2753 was able to grow from 15 to 40 °C and in NaCl concentrations of 0.5 to 9 % (w/v). The predominant fatty acids were 16 : 1ω7/16 : 1ω6 (27.9 %), 16 : 0 (22.1 %) and 18 : 1ω7/8 : 1ω6c (21.4 %). The genomic DNA G+C mol content was 49.5 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic differences, strain S2753 is considered to represent a novel species of the genus . Furthermore, whole genome sequence analysis comparing S2753 and type-strains of closely related species of the genus also demonstrated that the strain is genomically distinct enough to be considered a novel species. The name is proposed and the type-strain is S2753( = LMG 28894 = DSM 100496).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000603
2015-12-01
2020-09-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4503.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000603&mimeType=html&fmt=ahah

References

  1. Auch A. F., von Jan M., Klenk H. -P., Göker M., Digital D. N. A- D. N. A. ( 2010a;). hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2 117134 [CrossRef] [PubMed] .
    [Google Scholar]
  2. Auch A. F., Klenk H. -P., Göker M. ( 2010b;). Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2 142148 [CrossRef] [PubMed] .
    [Google Scholar]
  3. Beijerinck M. W. ( 1889;). Le Photobacterium luminosum, Bactérie lumineuse de la Mer du Nord. Arch Néerlandaises des Sci Exactes Nat 401427.
    [Google Scholar]
  4. Cowan S. T. ( 1974). Cowan and Steel's Manual for the Identification of Medical Bacteria , 2nd edn. Cambridge: Cambridge University Press;.
    [Google Scholar]
  5. Gabriel M. W., Matsui G. Y., Friedman R., Lovell C. R. ( 2014;). Optimization of multilocus sequence analysis for identification of species in the genus Vibrio . Appl Environ Microbiol 80 53595365 [CrossRef] [PubMed] .
    [Google Scholar]
  6. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57 8191 [CrossRef] [PubMed] .
    [Google Scholar]
  7. Gram L., Melchiorsen J., Bruhn J. B. ( 2010;). Antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms. Mar Biotechnol (NY) 12 439451 [CrossRef] [PubMed] .
    [Google Scholar]
  8. Gregersen T. ( 1978;). Rapid method for distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5 123127 [CrossRef].
    [Google Scholar]
  9. Machado H., Gram L. ( 2015;). The fur gene as a new phylogenetic marker for Vibrionaceae species identification. Appl Environ Microbiol 81 27452752 [CrossRef] [PubMed] .
    [Google Scholar]
  10. Machado H., Månsson M., Gram L. ( 2014;). Draft genome sequence of Photobacterium halotolerans S2753, producer of bioactive secondary metabolites. Genome Announc 2 e0053514 [CrossRef] [PubMed] .
    [Google Scholar]
  11. Mansson M., Gram L., Larsen T. O. ( 2011a;). Production of bioactive secondary metabolites by marine vibrionaceae . Mar Drugs 9 14401468 [CrossRef] [PubMed] .
    [Google Scholar]
  12. Mansson M., Nielsen A., Kjærulff L., Gotfredsen C. H., Wietz M., Ingmer H., Gram L., Larsen T. O. ( 2011b;). Inhibition of virulence gene expression in Staphylococcus aureus by novel depsipeptides from a marine photobacterium . Mar Drugs 9 25372552 [CrossRef] [PubMed] .
    [Google Scholar]
  13. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14 60 [CrossRef] [PubMed] .
    [Google Scholar]
  14. Sambrook J., Russel D. W. ( 2001). Molecular Cloning: A Laboratory Manual Cold Spring Harbour, New York: Cold Spring Harbour Laboratory Press;.
    [Google Scholar]
  15. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [CrossRef] [PubMed] .
    [Google Scholar]
  16. Wietz M., Mansson M., Gotfredsen C. H., Larsen T. O., Gram L. ( 2010;). Antibacterial compounds from marine Vibrionaceae isolated on a global expedition. Mar Drugs 8 29462960 [CrossRef] [PubMed] .
    [Google Scholar]
  17. ZoBell C. E. ( 1941;). Studies on Marine Bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4 4275.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000603
Loading
/content/journal/ijsem/10.1099/ijsem.0.000603
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error