1887

Abstract

Five Gram-negative, non-motile, rod-shaped bacterial strains were isolated from cankers of collected from different locations in Puyang city, Henan Province, China. The five strains were characterized by nutritional and physiological testing and DNA sequence analysis. Haemolysis was not observed on agar media supplemented with sheep erythrocytes. The strains could be distinguished from members of most species of the genus by their inability to assimilate -arginine and benzoate. The five strains formed a single branch in phylogenetic trees based on 16S rRNA, and individual gene sequence analysis, indicating that they all belonged to a single taxon within the genus . DNA–DNA hybridization results indicated that the five isolates represented to a single species that was separate from . On the basis of the phenotypic, genotypic and phylogenetic characteristics, the five strains are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of sp. nov. is PBJ7 (CFCC 11170 = KCTC 42272).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000599
2015-12-01
2019-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4461.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000599&mimeType=html&fmt=ahah

References

  1. Baker G. C. , Smith J. J. , Cowan D. A. . ( 2003;). Review and re-analysis of domain-specific 16S primers. J Microbiol Methods 55: 541–555 [CrossRef] [PubMed].
    [Google Scholar]
  2. Cleenwerck I. , Vandemeulebroecke K. , Janssens D. , Swings J. . ( 2002;). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov. Int J Syst Evol Microbiol 52: 1551–1558 [PubMed].
    [Google Scholar]
  3. Cruze J. A. , Singer J. T. , Finnerty W. R. . ( 1979;). Conditions for quantitative transformation in Acinetobacter calcoaceticus . Curr Microbiol 3: 129–132 [CrossRef].
    [Google Scholar]
  4. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224–229 [CrossRef].
    [Google Scholar]
  5. Krizova L. , Maixnerova M. , Sedo O. , Nemec A. . ( 2014;). Acinetobacter bohemicus sp. nov. widespread in natural soil and water ecosystems in the Czech Republic. Syst Appl Microbiol 37: 467–473 [CrossRef] [PubMed].
    [Google Scholar]
  6. Krizova L. , McGinnis J. , Maixnerova M. , Nemec M. , Poirel L. , Mingle L. , Sedo O. , Wolfgang W. , Nemec A. . ( 2015;). Acinetobacter variabilis sp. nov. (formerly DNA group 15 sensu Tjernberg & Ursing), isolated from humans and animals. Int J Syst Evol Microbiol 65: 1395 [CrossRef] [PubMed].
    [Google Scholar]
  7. Lane D. J. . ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. , Goodfellow M. . Chichester: Wiley;.
    [Google Scholar]
  8. Li Y. , Piao C. G. , Ma Y. C. , He W. , Wang H. M. , Chang J. P. , Guo L. M. , Wang X. Z. , Xie S. J. , Guo M. W. . ( 2013;). Acinetobacter puyangensis sp. nov., isolated from the healthy and diseased part of Populus ×  euramericana canker bark. Int J Syst Evol Microbiol 63: 2963–2969 [CrossRef] [PubMed].
    [Google Scholar]
  9. Li Y. , He W. , Wang T. , Piao C. G. , Guo L. M. , Chang J. P. , Guo M. W. , Xie S. J. . ( 2014a;). Acinetobacter qingfengensis sp. nov., isolated from canker bark of Populus ×  euramericana . Int J Syst Evol Microbiol 64: 1043–1050 [CrossRef] [PubMed].
    [Google Scholar]
  10. Li Y. , He W. , Ren F. J. , Guo L. M. , Chang J. P. , Cleenwerck I. , Ma Y. C. , Wang H. M. . ( 2014b;). A canker disease of Populus ×  euramericana in China caused by Lonsdalea quercina subsp. populi . Plant Dis 98: 368–378 [CrossRef].
    [Google Scholar]
  11. Marmur J. . ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208–218 [CrossRef].
    [Google Scholar]
  12. Marmur J. , Doty P. . ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118 [CrossRef] [PubMed].
    [Google Scholar]
  13. Nemec A. , Musílek M. , Maixnerová M. , De Baere T. , van der Reijden T.J.K. , Vaneechoutte M. , Dijkshoorn L. . ( 2009;). Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans. Int J Syst Evol Microbiol 59: 118–124 [CrossRef] [PubMed].
    [Google Scholar]
  14. Nemec A. , Krizova L. , Maixnerova M. , van der Reijden T. J. , Deschaght P. , Passet V. , Vaneechoutte M. , Brisse S. , Dijkshoorn L. . ( 2011;). Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). Res Microbiol 162: 393–404 [CrossRef] [PubMed].
    [Google Scholar]
  15. Smet A. , Cools P. , Krizova L. , Maixnerova M. , Sedo O. , Haesebrouck F. , Kempf M. , Nemec A. , Vaneechoutte M. . ( 2014;). Acinetobacter gandensis sp. nov. isolated from horse and cattle. Int J Syst Evol Microbiol 64: 4007–4015 [CrossRef] [PubMed].
    [Google Scholar]
  16. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  17. Touchon M. , Cury J. , Yoon E. J. , Krizova L. , Cerqueira G. C. , Murphy C. , Feldgarden M. , Wortman J. , Clermont D. , other authors . ( 2014;). The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences. Genome Biol Evol 6: 2866–2882 [CrossRef] [PubMed].
    [Google Scholar]
  18. Wayne L. G. , Brenner D. J. , Colwell R. R. . & other authors ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 297: 463–464.[CrossRef]
    [Google Scholar]
  19. Yamamoto S. , Harayama S. . ( 1995;). PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61: 1104–1109 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000599
Loading
/content/journal/ijsem/10.1099/ijsem.0.000599
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error