1887

Abstract

An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2, was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2 grew at 53–76 °C and at pH 5.9–8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C and C. Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1--phosphoinositol (diolPI), 2-acylalkyldiol-1--phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1--phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1--phosphoinositol mannoside (diolPI-Man). Strain WKT50.2 utilized a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilize monosaccharides. Robust growth of WKT50.2 was observed on protein derivatives. WKT50.2 was sensitive to ampicillin, chloramphenicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analysis of 16S rRNA gene sequences showed that WKT50.2 belonged to the class within the phylum , and was most closely related to KI4 (99.6% similarity). DNA–DNA hybridization between WKT50.2 and DSM 27169 was 18.0%. Physiological and biochemical tests confirmed the phenotypic and genotypic differentiation of strain WKT50.2 from KI4 and other members of the . On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain WKT50.2 represents a novel species, for which the name sp. nov. is proposed, with the type strain WKT50.2 ( = DSM 26011 = ICMP 20042). Emended descriptions of , , and are also proposed, and include the description of a novel respiratory quinone, MK-8 2,3-epoxide (23%), in .

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2015-12-01
2019-08-22
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