1887

Abstract

Two strains of a novel cactophilic yeast species were isolated from the columnar cactus in a sand dune ecosystem in Rio de Janeiro, Brazil. Phylogenetic analysis of sequences of the large subunit rRNA gene D1/D2 domains showed that the strains represent a sister species to , from which it differs by 21 nt substitutions and two indels. The novel species is heterothallic and the asci are deliquescent with the formation of two to four hat-shaped ascospores. The name sp. nov. is proposed for the species. The type strain is UFMG-CM-Y316 ( = CBS 13266) and the allotype is UFMG-CM-Y346a ( = CBS 13265). The Mycobank number is MB 810683. In addition, belonging to the clade, is reassigned to as a new combination.

Funding
This study was supported by the:
  • , Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq), Brazil , (Award 560715/2010-2)
  • , Fundação do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) , (Award 562292/2010-1)
  • , Natural Science and Engineering Research Council of Canada , (Award 307015/2013-0)
  • , Fundação para a Ciência e a Tecnologia, Portugal , (Award APQ-02163-11)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000596
2015-12-01
2020-09-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4474.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000596&mimeType=html&fmt=ahah

References

  1. Kurtzman C. P. ( 2011;). Starmera Y. Yamada, Higashi, Ando & Mikata (1997). . In The Yeasts: a Taxonomic Study , 5th edn.., pp. 805810. Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef].
    [Google Scholar]
  2. Kurtzman C. P., Robnett C. J. ( 2003;). Phylogenetic relationships among yeasts of the ‘Saccharomyces complex’ determined from multigene sequence analyses. FEMS Yeast Res 3 417432 [CrossRef] [PubMed] .
    [Google Scholar]
  3. Kurtzman C. P., Robnett C. J., Basehoar-Powers E. ( 2008;). Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 8 939954 [CrossRef] [PubMed] .
    [Google Scholar]
  4. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. ( 2011;). Methods for the isolation, phenotypic characterization and maintenance of yeasts. . In The Yeasts: a Taxonomic Study , 5th edn.., pp. 87110, Edited by Kurtzman C. P., Fell J. W., Boekhout T. Amsterdam: Elsevier; [CrossRef].
    [Google Scholar]
  5. Lachance M. A. ( 2012;). In defense of yeast sexual life cycles: the forma asexualis – an informal proposal. Yeast Newsletter 61 2425.
    [Google Scholar]
  6. Lachance M. A., Starmer W. T., Phaff H. J. ( 1988;). Identification of yeasts found in decaying cactus tissue. Can J Microbiol 34 10251036 [CrossRef].
    [Google Scholar]
  7. Lachance M. A., Bowles J. M., Starmer W. T., Barker J.S.F. ( 1999;). Kodamaea kakaduensis and Candida tolerans, two new ascomycetous yeast species from Australian Hibiscus flowers. Can J Microbiol 45 172177 [CrossRef] [PubMed] .
    [Google Scholar]
  8. Lachance M. A., Kaden J. E., Phaff H. J., Starmer W. T. ( 2001;). Phylogenetic structure of the Sporopachydermia cereana species complex. Int J Syst Evol Microbiol 51 237247 [CrossRef] [PubMed] .
    [Google Scholar]
  9. McNeill J. ( 2012;). International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). . In Regnum Veg 154 Koenigstein, Germany: A.R.G. Gantner Verlag;.
    [Google Scholar]
  10. Morais P. B., Rosa C. A., Hagler A. N., Mendonça-Hagler L. C. ( 1994;). Yeast communities of the cactus Pilosocereus arrabidae as resources for larval and adult stages of Drosophila serido . Antonie van Leeuwenhoek 66 313317 [CrossRef] [PubMed] .
    [Google Scholar]
  11. Phaff H. J., Starmer W. T., Lachance M. A., Aberdeen V., Tredick-Kline J. ( 1992;). Pichia caribaea, a new species of yeast occurring in necrotic tissue of cacti in the Caribbean area. Int J Syst Bacteriol 42 459462 [CrossRef].
    [Google Scholar]
  12. Rosa C. A., Hagler A. N., Mendonça-Hagler L.C.S., de Morais P. B., Gomes C. M., Monteiro R. F. ( 1992;). Clavispora opuntiae and other yeasts associated with the moth Sigelgaita sp. in the cactus Pilosocereus arrabidae of Rio de Janeiro. Brazil. Antonie van Leeuwenhoek 62 267272 [CrossRef] [PubMed] .
    [Google Scholar]
  13. Rosa C. A., Morais P. B., Hagler A. N., Mendonça-Hagler L. C., Monteiro R. F. ( 1994;). Yeast communities of the cactus Pilosocereus arrabidae and associated insects in the Sandy coastal plains of southeastern Brazil. Antonie van Leeuwenhoek 65 5562 [CrossRef] [PubMed] .
    [Google Scholar]
  14. Rosa C. A., Morais P. B., Santos S. R., Peres Neto P. R., Mendonça-Hagler L. C., Hagler A. N. ( 1995;). Yeast communities associated with different plant resources in sandy coastal plains of southeastern Brazil. Mycol Res 99 10471054 [CrossRef].
    [Google Scholar]
  15. Starmer W. T., Phaff H. J., Miranda M., Miller M. W. ( 1978;). Pichia amethionina, a new heterothallic yeast associated with the decaying stems of cereoid cacti. Int J Syst Bacteriol 28 433441 [CrossRef].
    [Google Scholar]
  16. Starmer W. T., Lachance M. A., Phaff H. J., Heed W. B. ( 1990;). The biogeography of yeasts associated with decaying cactus tissue in North America, the Caribbean, and Northern Venezuela. Evol Biol 24 253296.
    [Google Scholar]
  17. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [CrossRef] [PubMed] .
    [Google Scholar]
  18. Wickerham L. J., Burton K. A. ( 1954;). A simple technique for obtaining mating types in heterothallic diploid yeasts, with special reference to their uses in the genus Hansenula . J Bacteriol 67 303308 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000596
Loading
/content/journal/ijsem/10.1099/ijsem.0.000596
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error