sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment Free

Abstract

A Gram-staining-positive, moderately halophilic bacterium, designated strain NGS-2, was isolated from sediment of a solar saltern pond located in Shinan, Korea. Strain NGS-2 was a strictly aerobic, non-motile rod that grew at pH 5.0–10.0 (optimum, pH 8.0), at 10–30 °C (optimum, 28 °C) and in the presence of 1–20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain NGS-2 belonged to the genus , with sequence similarity of 98.4–95.8 % to existing type strains, showing the highest sequence similarity to D-8 (98.4 %), SL-4 (98.4 %), SL-5 (98.2 %), IGA7-4 (98.2 %), IS-Hb4 (98.1 %) and MS10 (98.0 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl--methylethanolamine and an unknown glycolipid. The cell-wall peptidoglycan was based on -Orn–-Asp, the predominant isoprenoid quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C and anteiso-C. The DNA G+C content of the novel isolate was 45.0 mol%. Levels of DNA–DNA relatedness between strain NGS-2 and the type strains of 12 other species of the genus ranged from 32 to 3 %. On the basis of the polyphasic analysis conducted in this study, strain NGS-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NGS-2 ( = KACC 18263 = NBRC 110639).

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2015-12-01
2024-03-28
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