1887

Abstract

A Gram-staining-positive, moderately halophilic bacterium, designated strain NGS-2, was isolated from sediment of a solar saltern pond located in Shinan, Korea. Strain NGS-2 was a strictly aerobic, non-motile rod that grew at pH 5.0–10.0 (optimum, pH 8.0), at 10–30 °C (optimum, 28 °C) and in the presence of 1–20 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain NGS-2 belonged to the genus , with sequence similarity of 98.4–95.8 % to existing type strains, showing the highest sequence similarity to D-8 (98.4 %), SL-4 (98.4 %), SL-5 (98.2 %), IGA7-4 (98.2 %), IS-Hb4 (98.1 %) and MS10 (98.0 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyl--methylethanolamine and an unknown glycolipid. The cell-wall peptidoglycan was based on -Orn–-Asp, the predominant isoprenoid quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C and anteiso-C. The DNA G+C content of the novel isolate was 45.0 mol%. Levels of DNA–DNA relatedness between strain NGS-2 and the type strains of 12 other species of the genus ranged from 32 to 3 %. On the basis of the polyphasic analysis conducted in this study, strain NGS-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NGS-2 ( = KACC 18263 = NBRC 110639).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000595
2015-12-01
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4434.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000595&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Malekzadeh F., Malik K. A., Schumann P., Spröer C. ( 2003;). Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53 10591063 [CrossRef] [PubMed].
    [Google Scholar]
  2. An S.-Y., Kanoh K., Kasai H., Goto K., Yokota A. ( 2007;). Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan. Int J Syst Evol Microbiol 57 24762479 [CrossRef] [PubMed].
    [Google Scholar]
  3. Chen Y. G., Liu Z. X., Zhang Y. Q., Zhang Y. X., Tang S. K., Borrathybay E., Li W. J., Cui X. L. ( 2009;). Halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone. Antonie van Leeuwenhoek 96 99107 [CrossRef] [PubMed].
    [Google Scholar]
  4. Claus D., Fahmy F., Rolf H. J., Tosunoglu N. ( 1983;). Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Microbiol 4 496506 [CrossRef] [PubMed].
    [Google Scholar]
  5. Collins M. D., Jones D. ( 1981;). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45 316354 [PubMed].
    [Google Scholar]
  6. Cowan S. T., Steel K. J. ( 1965). Manual for the Identification of Medical Bacteria London: Cambridge University Press;.
    [Google Scholar]
  7. DeLong E. F. ( 1992;). Archaea in coastal marine environments. Proc Natl Acad Sci U S A 89 56855689 [CrossRef] [PubMed].
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [CrossRef].
    [Google Scholar]
  9. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed].
    [Google Scholar]
  10. Felsenstein J. ( 1985;). Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39 783791 [CrossRef].
    [Google Scholar]
  11. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [CrossRef].
    [Google Scholar]
  12. Hua N.-P., Kanekiyo A., Fujikura K., Yasuda H., Naganuma T. ( 2007;). Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. Int J Syst Evol Microbiol 57 12431249 [CrossRef] [PubMed].
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed].
    [Google Scholar]
  14. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [CrossRef] [PubMed].
    [Google Scholar]
  15. Lányi B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 167 [CrossRef].
    [Google Scholar]
  16. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., other authors. ( 2007;). clustal w clustal x version 2.0. Bioinformatics 23 29472948 [CrossRef] [PubMed].
    [Google Scholar]
  17. Leifson E. ( 1963;). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85 11831184 [PubMed].
    [Google Scholar]
  18. Liu W. Y., Zeng J., Wang L., Dou Y. T., Yang S. S. ( 2005;). Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang. China. Int J Syst Evol Microbiol 55 19911996 [CrossRef] [PubMed].
    [Google Scholar]
  19. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 21142121 [CrossRef] [PubMed].
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [CrossRef].
    [Google Scholar]
  21. Murray R. G. E., Doetsch R. N., Robinow F. ( 1994;). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  22. Nunes I., Tiago I., Pires A. L., da Costa M. S., Veríssimo A. ( 2006;). Paucisalibacillus globulus gen. nov., sp. nov., a Gram-positive bacterium isolated from potting soil. Int J Syst Evol Microbiol 56 18411845 [CrossRef] [PubMed].
    [Google Scholar]
  23. Parte A. C. http://www.bacterio.net ( 2014). List of prokaryotic names with standing in nomenclature.
    [Google Scholar]
  24. Romano I., Finore I., Nicolaus G., Huertas F. J., Lama L., Nicolaus B., Poli A. ( 2008;). Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 58 886890 [CrossRef] [PubMed].
    [Google Scholar]
  25. Saito H., Miura K. ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72 619629 [CrossRef] [PubMed].
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  27. Schleifer K. H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407477 [PubMed].
    [Google Scholar]
  28. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. ( 1997;). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47 289298 [CrossRef] [PubMed].
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  30. Soto-Ramírez N., Sánchez-Porro C., Rosas-Padilla S., Almodóvar K., Jiménez G., Machado-Rodríguez M., Zapata M., Ventosa A., Montalvo-Rodríguez R. ( 2008;). Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans . Int J Syst Evol Microbiol 58 125130 [CrossRef] [PubMed].
    [Google Scholar]
  31. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. ( 1996;). Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46 492496 [CrossRef].
    [Google Scholar]
  32. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125128 [CrossRef].
    [Google Scholar]
  33. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [CrossRef] [PubMed].
    [Google Scholar]
  34. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464 [CrossRef].
    [Google Scholar]
  35. Yabuuchi E., Yano I., Oyaizu H., Hashimoto Y., Ezaki T., Yamamoto H. ( 1990;). Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas . Microbiol Immunol 34 99119 [CrossRef] [PubMed].
    [Google Scholar]
  36. Yabuuchi E., Kosako Y., Naka T., Suzuki S., Yano I. ( 1999;). Proposal of Sphingomonas suberifaciens (van Bruggen. Jochimsen and Brown 1990) comb. nov., Sphingomonas natatoria (Sly 1985) comb. nov., Sphingomonas ursincola (Yurkov et al. 1997) comb. nov., and emendation of the genus Sphingomonas. Microbiol Immunol 43 339349 [CrossRef] [PubMed].
    [Google Scholar]
  37. Yoon J.-H., Weiss N., Lee K. C., Lee I. S., Kang K. H., Park Y.-H. ( 2001;). Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen nov., comb. nov. Int J Syst Evol Microbiol 51 20872093 [CrossRef] [PubMed].
    [Google Scholar]
  38. Yoon J.-H., Kang K. H., Park Y.-H. ( 2003;). Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53 687693 [CrossRef] [PubMed].
    [Google Scholar]
  39. Yoon J.-H., Kang K. H., Oh T.-K., Park Y.-H. ( 2004;). Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8 2328 [CrossRef] [PubMed].
    [Google Scholar]
  40. Yoon J.-H., Kang S. J., Lee C. H., Oh H. W., Oh T. K. ( 2005;). Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55 24132417 [CrossRef] [PubMed].
    [Google Scholar]
  41. Yoon J.-H., Kang S.-J., Jung Y.-T., Oh T.-K. ( 2007;). Halobacillus campisalis sp. nov., containing meso-diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus . Int J Syst Evol Microbiol 57 20212025 [CrossRef] [PubMed].
    [Google Scholar]
  42. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2008;). Halobacillus seohaensis sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 58 622627 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000595
Loading
/content/journal/ijsem/10.1099/ijsem.0.000595
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error