1887

Abstract

A Gram-stain-negative, non-spore-forming, rod-shaped and aerobic bacterium, designated Xi19, was isolated from a soil sample collected from the rhizosphere of sunflower () in Wuyuan county of Inner Mongolia, China and was characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolate was related to species of the genus , sharing the greatest 16S rRNA gene sequence similarity with J3-AN59 (98.4 %), followed by J3-A127 (97.4 %). There were low similarities ( < 91 %) between the , and gene sequences of the novel strain and those of members of the genus . DNA–DNA hybridization values between strain Xi19 and the most related strain J3-AN59 were low. The major cellular fatty acids of strain Xi19 were C, summed feature 8 (Cω7 and/or Cω6) and C cyclo ω8. Q-10 was identified as the predominant ubiquinone and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The DNA G+C content of strain Xi19 was 60.2 mol%. On the basis of physiological and biochemical characteristics, coupled with genotypic data obtained in this work, strain Xi19 represents a novel species of the genus , for which the name is proposed. The type strain is Xi19 ( = CGMCC 1.12192 = KCTC 23879).

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2015-12-01
2020-01-21
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