1887

Abstract

A beige-pigmented bacterial strain (JM-310), isolated from the healthy internal root tissue of 4-week-old cotton (, cultivar ‘DES-119’) in Tallassee (Macon county), Alabama, USA, was studied taxonomically. The isolate produced small rod-shaped cells, which showed a Gram-negative staining behaviour. A comparison of the 16S rRNA gene sequence of the isolate revealed 99.2, 98.8, 98.7, 98.7, 98.1 and 97.6 % similarity to the 16S rRNA gene sequences of the type strains of , , , , and , respectively. In phylogenetic trees based on 16S rRNA gene sequences, strain JM-301 was placed within the monophyletic cluster of species. The fatty acid profile of strain JM-310 consisted mainly of the major fatty acids C, C 3-OH and summed feature 4 (iso-C 2-OH/Cω7/). The quinone system of strain JM-310 contained predominantly ubiquinone Q-8 and lesser amounts of Q-7 and Q-9. The major polyamine was putrescine and the diagnostic polyamine 2-hydroxyputrescine was detected as well. The polar lipid profile consisted of the major lipids phosphatidylethanolamine, phosphatidylglycerol, diphospatidylglycerol and several unidentified lipids. DNA–DNA hybridization experiments with LMG 1797, 1.22, KACC 11579 and 3165 gave levels of relatedness of < 70 %. These DNA–DNA hybridization results in addition to differential biochemical properties indicate clearly that strain JM-310 is a member of a novel species, for which the name sp. nov. is proposed. The type strain is JM-310 ( = LMG 28869 = CIP 110912 = CCM 8614).

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2015-12-01
2020-01-18
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