1887

Abstract

A Gram-staining-negative, non-gliding, orange-pigmented bacterial strain, designated HMF2925, was isolated from fresh water in Korea. The phylogenetic tree based on 16S rRNA gene sequences showed that strain HMF2925 formed a distinct lineage within the genus . Strain HMF2925 was closely related to DSM 17448 (95.5 %) and Gsoil 085 (94.1 %). The major fatty acids of strain HMF2925 were summed feature 3 (Cω6 and/or Cω7), iso-C, Cω5 and C.The major polar lipids of strain HMF2925 were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, two unidentified amino lipids and three unidentified polar lipids. The DNA G+C content of strain HMF2925 was 36.5 mol%. On the basis of the evidence presented in this study, strain HMF2925 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HMF2925 ( = KCTC 42574 = CECT 8858).

Funding
This study was supported by the:
  • National Institute of Biological Resources (Award no. 2014R1A6A301009795)
  • Ministry of Education
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2015-12-01
2024-10-06
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References

  1. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [PubMed].
    [Google Scholar]
  2. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed] .
    [Google Scholar]
  3. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  4. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  5. Gonzalez J. M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773 [View Article] [PubMed] .
    [Google Scholar]
  6. Joung Y., Lee B. I., Kang H., Kim H., Joh K. ( 2014;). Chitinimonas viridis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 64 11231126 [View Article] [PubMed] .
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  8. Liu Q. M., Ten L. N., Yu H. S., Jin F. X., Im W. T., Lee S. T. ( 2008;). Emticicia ginsengisoli sp. nov., a species of the family ‘Flexibacteraceae’ isolated from soil of a ginseng field. Int J Syst Evol Microbiol 58 11001105 [View Article] [PubMed] .
    [Google Scholar]
  9. Loganathan P., Vigneswaran S., Kandasamy J., Bolan N. S. ( 2014;). Removal and recovery of phosphate from water using sorption. Crit Rev Environ Sci Technol 44 847907 [View Article].
    [Google Scholar]
  10. Ludwig W., Euzéby J., Whitman W. B. ( 2010;). Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes., Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes . . In Bergey's Manual of Systematic Bacteriology, 4, pp. 2124. Edited by Krieg N. R., Ludwig W., Whitman W., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. New York: Springer;.
    [Google Scholar]
  11. MacFaddin J. F. ( 1980). Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore, MD: Williams & Wilkins;.
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  13. Saha P., Chakrabarti T. ( 2006;). Emticicia oligotrophica gen. nov., sp. nov., a new member of the family ‘Flexibacteraceae’, phylum Bacteroidetes . Int J Syst Evol Microbiol 56 991995 [View Article] [PubMed] .
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  15. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  16. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed] .
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P.A.D., Kandler O., Krichevsky M. I., Moore L. H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463464. [CrossRef]
    [Google Scholar]
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