1887

Abstract

An aerobic, endospore-forming, alkali-tolerant, Gram-stain-positive, non-motile, rod-shaped bacterium, designated strain NIO-S10, was isolated from a surface water sample collected from the Godavari River, Kapileswarapuram, India. Colonies on nutrient agar were circular, 3-4 mm in diameter, creamish and raised after incubation for 36 h at 37 °C. Growth occurred at 20–40 °C, at pH 6–9 and in the presence of 0–2 % (w/v) NaCl. Strain NIO-S10 was positive for oxidase, caseinase, DNase, gelatinase, lipase and urease activities, and negative for catalase, aesculinase, amylase and cellulase activities. The fatty acids were dominated by branched and saturated fatty acids, with a high abundance of iso-C, anteiso-C and C.The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid, and MK-7 was the major menaquinone. The DNA G+C content of strain NIO-S10 was 54.4 mol%. A sequence similarity search based on 16S rRNA gene sequences indicated that Gsoil 1105 was the nearest phylogenetic neighbour to strain NIO-S10, with a pairwise sequence similarity of 94.9 %. Phylogenetic analysis showed that strain NIO-S10 was clustered with members of the genus and together with members of the genus . Based on phenotypic characteristics and phylogenetic inference, strain NIO-S10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NIO-S10 ( = KCTC 32289 = MTCC 12483).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000576
2015-12-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4363.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000576&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. ( 1990;). Basic local alignment search tool. J Mol Biol 215: 403–410 [CrossRef] [PubMed].
    [Google Scholar]
  2. Anil Kumar P., Aravind R., Francis K., Bhumika V., Ritika C., Priyashanth P., Srinivas T,N.R.. ( 2012;). Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family “Cyclobacteriaceae” with nitrate reducing activity. Syst Appl Microbiol 35: 320–325 [CrossRef] [PubMed].
    [Google Scholar]
  3. Baek S. H., Cui Y., Kim S. C., Cui C. H., Yin C., Lee S. T., Im W. T.. ( 2011;). Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 61: 1715–1719 [CrossRef] [PubMed].
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100: 221–230 [CrossRef] [PubMed].
    [Google Scholar]
  5. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791 [CrossRef].
    [Google Scholar]
  6. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47: 1129–1133 [CrossRef] [PubMed].
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62: 716–721 [CrossRef] [PubMed].
    [Google Scholar]
  8. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19: 161–207 [CrossRef].
    [Google Scholar]
  9. Lane D. J.. ( 1991;). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M.. Chichester: Wiley;.
    [Google Scholar]
  10. Lányi B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19: 1–67 [CrossRef].
    [Google Scholar]
  11. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3: 208–218 [CrossRef].
    [Google Scholar]
  12. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425 [PubMed].
    [Google Scholar]
  13. Sasser M.. ( 1990;). Identification of bacteria through fatty acid analysis. . In Methods in Phytobacteriology, pp. 199–204. Edited by Klement Z., Rudolph K., Sands D. C.. Budapest: Akademiai Kiado;.
    [Google Scholar]
  14. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V.. ( 1986;). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5: 139–156 [CrossRef].
    [Google Scholar]
  15. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  16. Srinivas T.N.R., Nageswara Rao S.S.S., Vishnu Vardhan Reddy P., Pratibha M. S., Sailaja B., Kavya B., Hara Kishore K., Begum Z., Singh S. M., Shivaji S.. ( 2009;). Bacterial diversity and bioprospecting for cold-active lipases, amylases and proteases, from culturable bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic. Curr Microbiol 59: 537–547 [CrossRef] [PubMed].
    [Google Scholar]
  17. Steven B., Chen M. Q., Greer C. W., Whyte L. G., Niederberger T. D.. ( 2008;). Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost. Int J Syst Evol Microbiol 58: 1497–1501 [CrossRef] [PubMed].
    [Google Scholar]
  18. Tamura K., Nei M., Kumar S.. ( 2004;). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci U S A 101: 11030–11035 [CrossRef] [PubMed].
    [Google Scholar]
  19. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739 [CrossRef] [PubMed].
    [Google Scholar]
  20. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., Swings J.. ( 1996;). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60: 407–438 [PubMed].
    [Google Scholar]
  21. Wang Q., Xie N., Qin Y., Shen N., Zhu J., Mi H., Huang R.. ( 2013;). Tumebacillus flagellatus sp. nov., an α-amylase/pullulanase-producing bacterium isolated from cassava wastewater. Int J Syst Evol Microbiol 63: 3138–3142 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000576
Loading
/content/journal/ijsem/10.1099/ijsem.0.000576
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error