1887

Abstract

A novel bacterial strain, designated S2-2, was isolated from the intestine of a sea cucumber in Xiapu, Fujian province, China. Strain S2-2 was found to be aerobic, Gram-stain-negative, pale yellow, rod-shaped, oxidase- and catalase-positive. Growth occurred at 15–36 °C (optimum, 25–32 °C), in the presence of 2–7% sea salt (w/v, optimum, 3–5%) and at pH 6–9 (optimum, pH 7.0). The isolate was able to hydrolyse gelatin, casein and DNA, but unable to degrade Tween 20, 40 and 80, starch and cellulose. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2-2 represented a member of the genus , with highest sequence similarity to a4 (96.9% similarity), followed by WJ-1 (96.5% similarity) and showed lower similarities (93.3–95.9%) with other members of the genus . The major cellular fatty acids were summed feature 3 (comprising Cω6/Cω7, 33.8%), iso-C (13.7%), iso-CG (8.6%), iso-C 3-OH (5.3%) and C 3-OH (5.1%). The DNA G+C content of the chromosomal DNA was determined to be 31.8 mol%. The respiratory quinone was determined to be MK-6. Phosphatidylethanolamine, phosphatidylglycerol, one unknown aminophospholipid, one unknown phospholipid, one unknown glycolipid and five unknown lipids were detected as major polar lipids. Hence, the combined genotypic and phenotypic data indicated that strain S2-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S2-2 ( = MCCC 1A09872 = LMG 27758).

Funding
This study was supported by the:
  • , Scientific Research Foundation of the Third Institute of Oceanography, SOA , (Award 14CZP034HJ08)
  • , Public Welfare Project of SOA , (Award 2012002)
  • , the Fund of National Infrastructure of Microbial Resources , (Award 201005032)
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2015-12-01
2020-09-29
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. ( 2002;). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology. New York: Wiley;.
  2. Bernardet J. F., Nakagawa Y., Holmes B., Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [PubMed].
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57 22592261 [CrossRef] [PubMed] .
    [Google Scholar]
  4. Collins M. D. ( 1985;). Isoprenoid quinone analysis in bacterial classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. London: Academic Press;.
    [Google Scholar]
  5. Dong X., Cai M. ( 2001). Determinative Manual for Routine Bacteriology Beijing: Scientific Press;.
    [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [CrossRef] [PubMed] .
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [CrossRef] [PubMed] .
    [Google Scholar]
  8. Li Y., Wei J., Yang C., Lai Q., Chen Z., Li D., Zhang H., Tian Y., Zheng W., Zheng T. ( 2013;). Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. Int J Syst Evol Microbiol 63 34813486 [CrossRef] [PubMed] .
    [Google Scholar]
  9. Liu C., Shao Z. ( 2005;). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55 11811186 [CrossRef] [PubMed] .
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39 159167 [CrossRef].
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [CrossRef].
    [Google Scholar]
  12. Oh Y. S., Kahng H. Y., Lee D. H., Lee S. B. ( 2012;). Tenacibaculum jejuense sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 62 414419 [CrossRef] [PubMed] .
    [Google Scholar]
  13. Rzhetsky A., Nei M. ( 1992;). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35 367375 [CrossRef] [PubMed] .
    [Google Scholar]
  14. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  15. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  16. Shieh W. Y., Chen Y. W., Chaw S. M., Chiu H. H. ( 2003;). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53 479484 [CrossRef] [PubMed] .
    [Google Scholar]
  17. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. ( 2001;). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51 16391652 [CrossRef] [PubMed] .
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [CrossRef] [PubMed] .
    [Google Scholar]
  19. Wakabayashi H., Hikida M., Masumura K. ( 1986;). Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36 396398 [CrossRef].
    [Google Scholar]
  20. Wang J. T., Chou Y. J., Chou J. H., Chen C. A., Chen W. M. ( 2008;). Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone Aiptasia pulchella . Int J Syst Evol Microbiol 58 761766 [CrossRef] [PubMed] .
    [Google Scholar]
  21. Zhang G., Yang Y., Yin X., Wang S. ( 2014;). Paracoccus pacificus sp. nov., isolated from the Western Pacific Ocean. Antonie van Leeuwenhoek 106 725731 [CrossRef] [PubMed] .
    [Google Scholar]
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