1887

Abstract

A Gram-stain-negative, aerobic, non-spore-forming bacterial strain, motile by gliding and with rod-shaped or ovoid cells, was isolated from water of an estuary environment at Hwajinpo, South Korea. The strain was designated HJM-19 and subjected to a polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain HJM-19 belongs to the genus . It exhibited 16S rRNA gene sequence similarity values of 97.2–98.1 % to the type strains of , , , and , and of 93.9–96.5 % to the type strains of the other species of the genus with validly published names. Strain HJM-19 contained MK-6 as the predominant menaquinone and anteiso-C, anteiso-Cω9 and C 2-OH as the major fatty acids. The major polar lipids of strain HJM-19 were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HJM-19 was 48.0 mol%, and its DNA–DNA relatedness values with the type strains of the five phylogenetically closely related species of the genus were 11–23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain HJM-19 is separated from other species of the genus . On the basis of the data presented, strain HJM-19 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HJM-19 ( = KCTC 42706 = NBRC 111224).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000567
2015-11-01
2020-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/11/4244.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000567&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A..), ( 1993;). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef].
    [Google Scholar]
  2. Baumann P., Baumann L.. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G.. Berlin: Springer;.
    [Google Scholar]
  3. Bernardet J.-F.. ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey's Manual of Systematic Bacteriology, 2nd edn.vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N. L., Brown D. R., Parte A.. New York: Springer;.
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52: 1049–1070 [PubMed].
    [Google Scholar]
  5. Bowman J.P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50: 1861–1868 [CrossRef] [PubMed].
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51: 1997–2006 [CrossRef] [PubMed].
    [Google Scholar]
  7. Cho S.-H., Chae S.-H., Cho M., Kim T.-U., Choi S., Han J.-H., Kim Y.T., Joung Y., Joh K., other authors. ( 2011;). Gramella gaetbulicola sp. nov., a member of the family Flavobacteriaceae isolated from foreshore soil. Int J Syst Evol Microbiol 61: 2654–2658 [CrossRef] [PubMed].
    [Google Scholar]
  8. Cohen-Bazire G., Sistrom W.R., Stanier R.Y.. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49: 25–68 [CrossRef] [PubMed].
    [Google Scholar]
  9. Embley T.M., Wait R.. ( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O'Donnell A. G.. Chichester: Wiley;.
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39: 224–229 [CrossRef].
    [Google Scholar]
  11. Hameed A., Shahina M., Lin S.-Y., Liu Y.-C., Lai W.-A., Young C.-C.. ( 2014;). Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 64: 2675–2681 [CrossRef] [PubMed].
    [Google Scholar]
  12. Jeong S.H., Jin H.M., Jeon C.O.. ( 2013;). Gramella aestuarii sp. nov., isolated from a tidal flat, and emended description of Gramella echinicola. Int J Syst Evol Microbiol 63: 2872–2878 [CrossRef] [PubMed].
    [Google Scholar]
  13. Komagata K., Suzuki K.. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19: 161–207 [CrossRef].
    [Google Scholar]
  14. Lányí B.. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19: 1–67 [CrossRef].
    [Google Scholar]
  15. Lau S.C.K., Tsoi M.M.Y., Li X., Plakhotnikova I., Dobretsov S., Wong P.-K., Qian P.-Y.. ( 2005;). Gramella portivictoriae sp. nov., a novel member of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 55: 2497–2500 [CrossRef] [PubMed].
    [Google Scholar]
  16. Liu K., Li S., Jiao N., Tang K.. ( 2014;). Gramella flava sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 64: 165–168 [CrossRef] [PubMed].
    [Google Scholar]
  17. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2: 233–241 [CrossRef].
    [Google Scholar]
  18. Nedashkovskaya O.I., Kim S.B., Lysenko A.M., Frolova G.M., Mikhailov V.V., Bae K.S., Lee D.H., Kim I.S.. ( 2005;). Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55: 391–394 [CrossRef] [PubMed].
    [Google Scholar]
  19. Nedashkovskaya O.I., Kim S.B., Bae K.S.. ( 2010;). Gramella marina sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 60: 2799–2802 [CrossRef] [PubMed].
    [Google Scholar]
  20. Park S., Park D.-S., Bae K.S., Yoon J.-H.. ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64: 1378–1383 [CrossRef] [PubMed].
    [Google Scholar]
  21. Park J.-M., Park S., Won S.-M., Jung Y.-T., Shin K.-S., Yoon J.-H.. ( 2015;). Gramella aestuariivivens sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 65: 1262–1267 [CrossRef] [PubMed].
    [Google Scholar]
  22. Reichenbach H.. ( 1992;). The order Cytophagales. . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn.vol. 4, pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H.. New York: Springer;.
    [Google Scholar]
  23. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  24. Shahina M., Hameed A., Lin S.-Y., Lee R.-J., Lee M.-R., Young C.-C.. ( 2014a;). Gramella planctonica sp. nov., a zeaxanthin-producing bacterium isolated from surface seawater, and emended descriptions of Gramella aestuarii and Gramella echinicola. Antonie van Leeuwenhoek 105: 771–779 [CrossRef] [PubMed].
    [Google Scholar]
  25. Shahina M., Hameed A., Lin S.-Y., Lee R.-J., Lee M.-R., Young C.-C.. ( 2014b;). Gramella planctonica sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 139. Int J Syst Evol Microbiol 64: 2184–2187 [CrossRef].
    [Google Scholar]
  26. Stackebrandt E., Goebel B.M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44: 846–849 [CrossRef].
    [Google Scholar]
  27. Staley J.T.. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95: 1921–1942 [PubMed].
    [Google Scholar]
  28. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25: 125–128 [CrossRef].
    [Google Scholar]
  29. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37: 463–464 [CrossRef].
    [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W.Y., Lee S.T., Goodfellow M., Park Y.-H.. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46: 502–505 [CrossRef].
    [Google Scholar]
  31. Yoon J.-H., Lee S.T., Park Y.-H.. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48: 187–194 [CrossRef] [PubMed].
    [Google Scholar]
  32. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K.H., Park Y.-H.. ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53: 53–57 [CrossRef] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000567
Loading
/content/journal/ijsem/10.1099/ijsem.0.000567
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error