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Volume 65, Issue Pt_11

sp. nov., isolated from water of an estuary environment Free

Abstract

A Gram-stain-negative, aerobic, non-spore-forming bacterial strain, motile by gliding and with rod-shaped or ovoid cells, was isolated from water of an estuary environment at Hwajinpo, South Korea. The strain was designated HJM-19 and subjected to a polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain HJM-19 belongs to the genus . It exhibited 16S rRNA gene sequence similarity values of 97.2–98.1 % to the type strains of , , , and , and of 93.9–96.5 % to the type strains of the other species of the genus with validly published names. Strain HJM-19 contained MK-6 as the predominant menaquinone and anteiso-C, anteiso-Cω9 and C 2-OH as the major fatty acids. The major polar lipids of strain HJM-19 were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain HJM-19 was 48.0 mol%, and its DNA–DNA relatedness values with the type strains of the five phylogenetically closely related species of the genus were 11–23 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain HJM-19 is separated from other species of the genus . On the basis of the data presented, strain HJM-19 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HJM-19 ( = KCTC 42706 = NBRC 111224).

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Funding
This study was supported by the:
  • project on survey of indigenous species of Korea of the National Institute of Biological Resources (Award NRF-2013M3A9A5075953)
  • Programs for Collection, Management and Utilization of Biological Resources from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea
© 2015 IUMS
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2015-11-01
2024-04-19
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References

  1. Barrow G. I., Feltham R. K. A. ), ( 1993). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press; [View Article].
     [Google Scholar]
  2. Baumann P., Baumann L. ( 1981;). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 1302–1331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;.
     [Google Scholar]
  3. Bernardet J.-F. ( 2011;). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 4, pp. 106–111. Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N. L., Brown D. R., Parte A. New York: Springer;.
     [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 1049–1070 [PubMed].
     [Google Scholar]
  5. Bowman J.P. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50 1861–1868 [View Article] [PubMed].
     [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51 1997–2006 [View Article] [PubMed].
     [Google Scholar]
  7. Cho S.-H., Chae S.-H., Cho M., Kim T.-U., Choi S., Han J.-H., Kim Y.T., Joung Y., Joh K., other authors. ( 2011;). Gramella gaetbulicola sp. nov., a member of the family Flavobacteriaceae isolated from foreshore soil. Int J Syst Evol Microbiol 61 2654–2658 [View Article] [PubMed].
     [Google Scholar]
  8. Cohen-Bazire G., Sistrom W.R., Stanier R.Y. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49 25–68 [View Article] [PubMed].
     [Google Scholar]
  9. Embley T.M., Wait R. ( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121–161. Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;.
     [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224–229 [View Article].
     [Google Scholar]
  11. Hameed A., Shahina M., Lin S.-Y., Liu Y.-C., Lai W.-A., Young C.-C. ( 2014;). Gramella oceani sp. nov., a zeaxanthin-producing bacterium of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 64 2675–2681 [View Article] [PubMed].
     [Google Scholar]
  12. Jeong S.H., Jin H.M., Jeon C.O. ( 2013;). Gramella aestuarii sp. nov., isolated from a tidal flat, and emended description of Gramella echinicola . Int J Syst Evol Microbiol 63 2872–2878 [View Article] [PubMed].
     [Google Scholar]
  13. Komagata K., Suzuki K. ( 1987;). Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19 161–207 [View Article].
     [Google Scholar]
  14. Lányí B. ( 1987;). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19 1–67 [View Article].
     [Google Scholar]
  15. Lau S.C.K., Tsoi M.M.Y., Li X., Plakhotnikova I., Dobretsov S., Wong P.-K., Qian P.-Y. ( 2005;). Gramella portivictoriae sp. nov., a novel member of the family Flavobacteriaceae isolated from marine sediment. Int J Syst Evol Microbiol 55 2497–2500 [View Article] [PubMed].
     [Google Scholar]
  16. Liu K., Li S., Jiao N., Tang K. ( 2014;). Gramella flava sp. nov., a member of the family Flavobacteriaceae isolated from seawater. Int J Syst Evol Microbiol 64 165–168 [View Article] [PubMed].
     [Google Scholar]
  17. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  18. Nedashkovskaya O.I., Kim S.B., Lysenko A.M., Frolova G.M., Mikhailov V.V., Bae K.S., Lee D.H., Kim I.S. ( 2005;). Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 55 391–394 [View Article] [PubMed].
     [Google Scholar]
  19. Nedashkovskaya O.I., Kim S.B., Bae K.S. ( 2010;). Gramella marina sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 60 2799–2802 [View Article] [PubMed].
     [Google Scholar]
  20. Park S., Park D.-S., Bae K.S., Yoon J.-H. ( 2014;). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64 1378–1383 [View Article] [PubMed].
     [Google Scholar]
  21. Park J.-M., Park S., Won S.-M., Jung Y.-T., Shin K.-S., Yoon J.-H. ( 2015;). Gramella aestuariivivens sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 65 1262–1267 [View Article] [PubMed].
     [Google Scholar]
  22. Reichenbach H. ( 1992;). The order Cytophagales . . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. vol. 4, pp. 3631–3675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;.
     [Google Scholar]
  23. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  24. Shahina M., Hameed A., Lin S.-Y., Lee R.-J., Lee M.-R., Young C.-C. ( 2014a;). Gramella planctonica sp. nov., a zeaxanthin-producing bacterium isolated from surface seawater, and emended descriptions of Gramella aestuarii and Gramella echinicola . Antonie van Leeuwenhoek 105 771–779 [View Article] [PubMed].
     [Google Scholar]
  25. Shahina M., Hameed A., Lin S.-Y., Lee R.-J., Lee M.-R., Young C.-C. ( 2014b;). Gramella planctonica sp. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 139. Int J Syst Evol Microbiol 64 2184–2187 [View Article].
     [Google Scholar]
  26. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44 846–849 [View Article].
     [Google Scholar]
  27. Staley J.T. ( 1968;). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95 1921–1942 [PubMed].
     [Google Scholar]
  28. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125–128 [View Article].
     [Google Scholar]
  29. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
  30. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W.Y., Lee S.T., Goodfellow M., Park Y.-H. ( 1996;). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46 502–505 [View Article].
     [Google Scholar]
  31. Yoon J.-H., Lee S.T., Park Y.-H. ( 1998;). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48 187–194 [View Article] [PubMed].
     [Google Scholar]
  32. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K.H., Park Y.-H. ( 2003;). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53 53–57 [View Article] [PubMed].
     [Google Scholar]
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Gramella aquimixticola sp. nov., isolated from water of an estuary environment
Int J Syst Evol Microbiol 65, 4244 (2015); https://doi.org/10.1099/ijsem.0.000567
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