1887

Abstract

A Gram-stain-positive, non-motile and aerobic bacterium, designated strain DW151B, was isolated from a sludge sample of a dairy industry effluent treatment plant. 16S rRNA gene sequence analysis of strain DW151B placed it within the genus . It displayed significant similarity with recognized species of the genus: PDB9 (98.8 %), W 4937 (98.6 %), DSM 43241 (98.5 %) and YIM 65754 (97.5 %). However, strain DW151B differed from phylogenetically closely related species in various phenotypic properties. The cellular polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) as major lipids, MK-8(H) was the major menaquinone and -diaminopimelic acid was the cell-wall peptidoglycan. The fatty acid profile consisted of C, C 9 and C 9 as main components. The presence of C and diphosphatidylglycerol as major fatty acid and polar lipid, respectively, was in accordance with chemotaxonomic markers of the genus . The DNA G+C content of strain DW151B was 69.9 mol%, a value within the limits reported for the members of this genus. Furthermore, strain DW151B showed low similarity at the whole genome level in DNA–DNA hybridization experiments with phylogenetically closely related strains. Considering the low similarity at the genome level and differences in phenotypic properties, strain DW151B is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DW151B ( = MTCC 12279 = DSM 45625).

Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology, Government of India
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2015-11-01
2021-10-28
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